Salivary dysbiosis in Sjögren’s syndrome and a commensal-mediated immunomodulatory effect of salivary gland epithelial cells

Abstract Salivary gland epithelial cells (SGECs) have been implicated in the pathogenesis of Sjögren’s syndrome due to aberrant antigen-presentation function. This study examined the hypothesis that oral dysbiosis modulates the antigen-presentation function of SGECs, which regulates CD4 T cell proli...

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Autores principales:	Yu-chao Tseng, Hsin-yi Yang, Wei-ting Lin, Chia-bin Chang, Hsiu-chuan Chien, Hon-pin Wang, Chun-ming Chen, Jann-tay Wang, Chin Li, Shu-fen Wu, Song-chou Hsieh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/68092dcf2ebf4f8184c1fc1790ef72fa
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spelling	oai:doaj.org-article:68092dcf2ebf4f8184c1fc1790ef72fa2021-12-02T13:19:32ZSalivary dysbiosis in Sjögren’s syndrome and a commensal-mediated immunomodulatory effect of salivary gland epithelial cells10.1038/s41522-021-00192-w2055-5008https://doaj.org/article/68092dcf2ebf4f8184c1fc1790ef72fa2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00192-whttps://doaj.org/toc/2055-5008Abstract Salivary gland epithelial cells (SGECs) have been implicated in the pathogenesis of Sjögren’s syndrome due to aberrant antigen-presentation function. This study examined the hypothesis that oral dysbiosis modulates the antigen-presentation function of SGECs, which regulates CD4 T cell proliferation in primary Sjögren’s syndrome (pSS). Saliva samples from 8 pSS patients and 16 healthy subjects were analyzed for bacterial 16S ribosomal DNA. As a result, 39 differentially abundant taxa were identified. Among them, the phylum Proteobacteria comprised 21 taxa, and this phylum was mostly enriched in the healthy controls. The proteobacterium Haemophilus parainfluenzae was enriched in the healthy controls, with the greatest effect size at the species level. Treatment of A253 cells in vitro with H. parainfluenzae upregulated PD-L1 expression, and H. parainfluenzae-pretreated A253 cells suppressed CD4 T cell proliferation. The suppression was partially reversed by PD-L1 blockade. Among low-grade xerostomia patients, salivary abundance of H. parainfluenzae decreased in pSS patients compared to that in non-pSS sicca patients. Our findings suggest that H. parainfluenzae may be an immunomodulatory commensal bacterium in pSS.Yu-chao TsengHsin-yi YangWei-ting LinChia-bin ChangHsiu-chuan ChienHon-pin WangChun-ming ChenJann-tay WangChin LiShu-fen WuSong-chou HsiehNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Yu-chao Tseng Hsin-yi Yang Wei-ting Lin Chia-bin Chang Hsiu-chuan Chien Hon-pin Wang Chun-ming Chen Jann-tay Wang Chin Li Shu-fen Wu Song-chou Hsieh Salivary dysbiosis in Sjögren’s syndrome and a commensal-mediated immunomodulatory effect of salivary gland epithelial cells
description	Abstract Salivary gland epithelial cells (SGECs) have been implicated in the pathogenesis of Sjögren’s syndrome due to aberrant antigen-presentation function. This study examined the hypothesis that oral dysbiosis modulates the antigen-presentation function of SGECs, which regulates CD4 T cell proliferation in primary Sjögren’s syndrome (pSS). Saliva samples from 8 pSS patients and 16 healthy subjects were analyzed for bacterial 16S ribosomal DNA. As a result, 39 differentially abundant taxa were identified. Among them, the phylum Proteobacteria comprised 21 taxa, and this phylum was mostly enriched in the healthy controls. The proteobacterium Haemophilus parainfluenzae was enriched in the healthy controls, with the greatest effect size at the species level. Treatment of A253 cells in vitro with H. parainfluenzae upregulated PD-L1 expression, and H. parainfluenzae-pretreated A253 cells suppressed CD4 T cell proliferation. The suppression was partially reversed by PD-L1 blockade. Among low-grade xerostomia patients, salivary abundance of H. parainfluenzae decreased in pSS patients compared to that in non-pSS sicca patients. Our findings suggest that H. parainfluenzae may be an immunomodulatory commensal bacterium in pSS.
format	article
author	Yu-chao Tseng Hsin-yi Yang Wei-ting Lin Chia-bin Chang Hsiu-chuan Chien Hon-pin Wang Chun-ming Chen Jann-tay Wang Chin Li Shu-fen Wu Song-chou Hsieh
author_facet	Yu-chao Tseng Hsin-yi Yang Wei-ting Lin Chia-bin Chang Hsiu-chuan Chien Hon-pin Wang Chun-ming Chen Jann-tay Wang Chin Li Shu-fen Wu Song-chou Hsieh
author_sort	Yu-chao Tseng
title	Salivary dysbiosis in Sjögren’s syndrome and a commensal-mediated immunomodulatory effect of salivary gland epithelial cells
title_short	Salivary dysbiosis in Sjögren’s syndrome and a commensal-mediated immunomodulatory effect of salivary gland epithelial cells
title_full	Salivary dysbiosis in Sjögren’s syndrome and a commensal-mediated immunomodulatory effect of salivary gland epithelial cells
title_fullStr	Salivary dysbiosis in Sjögren’s syndrome and a commensal-mediated immunomodulatory effect of salivary gland epithelial cells
title_full_unstemmed	Salivary dysbiosis in Sjögren’s syndrome and a commensal-mediated immunomodulatory effect of salivary gland epithelial cells
title_sort	salivary dysbiosis in sjögren’s syndrome and a commensal-mediated immunomodulatory effect of salivary gland epithelial cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/68092dcf2ebf4f8184c1fc1790ef72fa
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Salivary dysbiosis in Sjögren’s syndrome and a commensal-mediated immunomodulatory effect of salivary gland epithelial cells

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