Analysis of serum miRNA profiles of myasthenia gravis patients.

Myasthenia gravis (MG) is an autoimmune disease characterized by the presence of autoantibodies, mainly against the acetylcholine receptor (AChR). The mechanisms triggering and maintaining this chronic disease are unknown. MiRNAs are regulatory molecules that play a key role in the immune system and...

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Autores principales:	Gisela Nogales-Gadea, Alba Ramos-Fransi, Xavier Suárez-Calvet, Miquel Navas, Ricard Rojas-García, Jose Luis Mosquera, Jordi Díaz-Manera, Luis Querol, Eduard Gallardo, Isabel Illa
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/680a1d439c5c465e996a66ad2745698b
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spelling	oai:doaj.org-article:680a1d439c5c465e996a66ad2745698b2021-11-18T08:27:51ZAnalysis of serum miRNA profiles of myasthenia gravis patients.1932-620310.1371/journal.pone.0091927https://doaj.org/article/680a1d439c5c465e996a66ad2745698b2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24637658/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Myasthenia gravis (MG) is an autoimmune disease characterized by the presence of autoantibodies, mainly against the acetylcholine receptor (AChR). The mechanisms triggering and maintaining this chronic disease are unknown. MiRNAs are regulatory molecules that play a key role in the immune system and are altered in many autoimmune diseases. The aim of this study was to evaluate miRNA profiles in serum of 61 AChR MG patients. We studied serum from patients with early onset MG (n = 22), late onset MG (n = 27) and thymoma (n = 12), to identify alterations in the specific subgroups. In a discovery cohort, we analysed 381 miRNA arrays from 5 patients from each subgroup, and 5 healthy controls. The 15 patients had not received any treatment. We found 32 miRNAs in different levels in MG and analysed 8 of these in a validation cohort that included 46 of the MG patients. MiR15b, miR122, miR-140-3p, miR185, miR192, miR20b and miR-885-5p were in lower levels in MG patients than in controls. Our study suggests that different clinical phenotypes in MG share common altered mechanisms in circulating miRNAs, with no additional contribution of the thymoma. MG treatment intervention does not modify the profile of these miRNAs. Novel insights into the pathogenesis of MG can be reached by the analysis of circulating miRNAs since some of these miRNAs have also been found low in MG peripheral mononuclear cells, and have targets with important roles in B cell survival and antibody production.Gisela Nogales-GadeaAlba Ramos-FransiXavier Suárez-CalvetMiquel NavasRicard Rojas-GarcíaJose Luis MosqueraJordi Díaz-ManeraLuis QuerolEduard GallardoIsabel IllaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e91927 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gisela Nogales-Gadea Alba Ramos-Fransi Xavier Suárez-Calvet Miquel Navas Ricard Rojas-García Jose Luis Mosquera Jordi Díaz-Manera Luis Querol Eduard Gallardo Isabel Illa Analysis of serum miRNA profiles of myasthenia gravis patients.
description	Myasthenia gravis (MG) is an autoimmune disease characterized by the presence of autoantibodies, mainly against the acetylcholine receptor (AChR). The mechanisms triggering and maintaining this chronic disease are unknown. MiRNAs are regulatory molecules that play a key role in the immune system and are altered in many autoimmune diseases. The aim of this study was to evaluate miRNA profiles in serum of 61 AChR MG patients. We studied serum from patients with early onset MG (n = 22), late onset MG (n = 27) and thymoma (n = 12), to identify alterations in the specific subgroups. In a discovery cohort, we analysed 381 miRNA arrays from 5 patients from each subgroup, and 5 healthy controls. The 15 patients had not received any treatment. We found 32 miRNAs in different levels in MG and analysed 8 of these in a validation cohort that included 46 of the MG patients. MiR15b, miR122, miR-140-3p, miR185, miR192, miR20b and miR-885-5p were in lower levels in MG patients than in controls. Our study suggests that different clinical phenotypes in MG share common altered mechanisms in circulating miRNAs, with no additional contribution of the thymoma. MG treatment intervention does not modify the profile of these miRNAs. Novel insights into the pathogenesis of MG can be reached by the analysis of circulating miRNAs since some of these miRNAs have also been found low in MG peripheral mononuclear cells, and have targets with important roles in B cell survival and antibody production.
format	article
author	Gisela Nogales-Gadea Alba Ramos-Fransi Xavier Suárez-Calvet Miquel Navas Ricard Rojas-García Jose Luis Mosquera Jordi Díaz-Manera Luis Querol Eduard Gallardo Isabel Illa
author_facet	Gisela Nogales-Gadea Alba Ramos-Fransi Xavier Suárez-Calvet Miquel Navas Ricard Rojas-García Jose Luis Mosquera Jordi Díaz-Manera Luis Querol Eduard Gallardo Isabel Illa
author_sort	Gisela Nogales-Gadea
title	Analysis of serum miRNA profiles of myasthenia gravis patients.
title_short	Analysis of serum miRNA profiles of myasthenia gravis patients.
title_full	Analysis of serum miRNA profiles of myasthenia gravis patients.
title_fullStr	Analysis of serum miRNA profiles of myasthenia gravis patients.
title_full_unstemmed	Analysis of serum miRNA profiles of myasthenia gravis patients.
title_sort	analysis of serum mirna profiles of myasthenia gravis patients.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/680a1d439c5c465e996a66ad2745698b
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Analysis of serum miRNA profiles of myasthenia gravis patients.

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