Beneficial Effects of Neurotrophin-4 Supplementation During in vitro Maturation of Porcine Cumulus-Oocyte Complexes and Subsequent Embryonic Development After Parthenogenetic Activation
Neurotrophin-4 (NT-4) is a neurotrophic factor that plays an important role in follicular development and oocyte maturation. However, it is not yet known whether NT-4 is related to oocyte maturation and follicular development in pigs. This study aims to investigate the effects of NT-4 supplementatio...
Guardado en:
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Frontiers Media S.A.
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/6816925bd6534335969a681142e7f445 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:6816925bd6534335969a681142e7f445 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:6816925bd6534335969a681142e7f4452021-11-12T05:27:23ZBeneficial Effects of Neurotrophin-4 Supplementation During in vitro Maturation of Porcine Cumulus-Oocyte Complexes and Subsequent Embryonic Development After Parthenogenetic Activation2297-176910.3389/fvets.2021.779298https://doaj.org/article/6816925bd6534335969a681142e7f4452021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.779298/fullhttps://doaj.org/toc/2297-1769Neurotrophin-4 (NT-4) is a neurotrophic factor that plays an important role in follicular development and oocyte maturation. However, it is not yet known whether NT-4 is related to oocyte maturation and follicular development in pigs. This study aims to investigate the effects of NT-4 supplementation during in vitro maturation (IVM) of porcine oocytes and subsequent embryonic development after parthenogenetic activation (PA). First, NT-4 and its receptors (TrkB and p75NTR) were identified through fluorescent immunohistochemistry in porcine ovaries. NT-4 was mainly expressed in theca and granulosa cells; phospho-TrkB and total TrkB were expressed in theca cells, granulosa cells, and oocytes; p75NTR was expressed in all follicular cells. During IVM, the defined maturation medium was supplemented with various concentrations of NT-4 (0, 1, 10, and 100 ng/mL). After IVM, the nuclear maturation rate was significantly higher in the 10 and 100 ng/mL NT-4 treated groups than in the control. There was no significant difference in the intracellular reactive oxygen species levels in any group after IVM, but the 1 and 10 ng/mL NT-4 treatment groups showed a significant increase in the intracellular glutathione levels compared to the control. In matured cumulus cells, the 10 ng/mL NT-4 treatment group showed significantly increased cumulus expansion-related genes and epidermal growth factor (EGF) signaling pathway-related genes. In matured oocytes, the 10 ng/mL treatment group showed significantly increased expression of cell proliferation-related genes, antioxidant-related genes, and EGF signaling pathway-related genes. We also investigated the subsequent embryonic developmental competence of PA embryos. After PA, the cleavage rates significantly increased in the 10 and 100 ng/mL NT-4 treatment groups. Although there was no significant difference in the total cell number of blastocysts, only the 10 ng/mL NT-4 treatment group showed a higher blastocyst formation rate than the control group. Our findings suggest that supplementation with the 10 ng/mL NT-4 can enhance porcine oocyte maturation by interacting with the EGF receptor signaling pathway. In addition, we demonstrated for the first time that NT-4 is not only required for porcine follicular development, but also has beneficial effects on oocyte maturation and developmental competence of PA embryos.Mirae KimMirae KimSeon-Ung HwangSeon-Ung HwangJunchul David YoonJunchul David YoonJoohyeong LeeJoohyeong LeeEunhye KimEunhye KimLian CaiLian CaiGahye KimGahye KimHyerin ChoiHyerin ChoiDongjin OhDongjin OhSang-Hwan HyunSang-Hwan HyunSang-Hwan HyunFrontiers Media S.A.articleneurotrophin-4pigfollicular developmentin vitro maturation of oocytesepidermal growth factorVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
neurotrophin-4 pig follicular development in vitro maturation of oocytes epidermal growth factor Veterinary medicine SF600-1100 |
| spellingShingle |
neurotrophin-4 pig follicular development in vitro maturation of oocytes epidermal growth factor Veterinary medicine SF600-1100 Mirae Kim Mirae Kim Seon-Ung Hwang Seon-Ung Hwang Junchul David Yoon Junchul David Yoon Joohyeong Lee Joohyeong Lee Eunhye Kim Eunhye Kim Lian Cai Lian Cai Gahye Kim Gahye Kim Hyerin Choi Hyerin Choi Dongjin Oh Dongjin Oh Sang-Hwan Hyun Sang-Hwan Hyun Sang-Hwan Hyun Beneficial Effects of Neurotrophin-4 Supplementation During in vitro Maturation of Porcine Cumulus-Oocyte Complexes and Subsequent Embryonic Development After Parthenogenetic Activation |
| description |
Neurotrophin-4 (NT-4) is a neurotrophic factor that plays an important role in follicular development and oocyte maturation. However, it is not yet known whether NT-4 is related to oocyte maturation and follicular development in pigs. This study aims to investigate the effects of NT-4 supplementation during in vitro maturation (IVM) of porcine oocytes and subsequent embryonic development after parthenogenetic activation (PA). First, NT-4 and its receptors (TrkB and p75NTR) were identified through fluorescent immunohistochemistry in porcine ovaries. NT-4 was mainly expressed in theca and granulosa cells; phospho-TrkB and total TrkB were expressed in theca cells, granulosa cells, and oocytes; p75NTR was expressed in all follicular cells. During IVM, the defined maturation medium was supplemented with various concentrations of NT-4 (0, 1, 10, and 100 ng/mL). After IVM, the nuclear maturation rate was significantly higher in the 10 and 100 ng/mL NT-4 treated groups than in the control. There was no significant difference in the intracellular reactive oxygen species levels in any group after IVM, but the 1 and 10 ng/mL NT-4 treatment groups showed a significant increase in the intracellular glutathione levels compared to the control. In matured cumulus cells, the 10 ng/mL NT-4 treatment group showed significantly increased cumulus expansion-related genes and epidermal growth factor (EGF) signaling pathway-related genes. In matured oocytes, the 10 ng/mL treatment group showed significantly increased expression of cell proliferation-related genes, antioxidant-related genes, and EGF signaling pathway-related genes. We also investigated the subsequent embryonic developmental competence of PA embryos. After PA, the cleavage rates significantly increased in the 10 and 100 ng/mL NT-4 treatment groups. Although there was no significant difference in the total cell number of blastocysts, only the 10 ng/mL NT-4 treatment group showed a higher blastocyst formation rate than the control group. Our findings suggest that supplementation with the 10 ng/mL NT-4 can enhance porcine oocyte maturation by interacting with the EGF receptor signaling pathway. In addition, we demonstrated for the first time that NT-4 is not only required for porcine follicular development, but also has beneficial effects on oocyte maturation and developmental competence of PA embryos. |
| format |
article |
| author |
Mirae Kim Mirae Kim Seon-Ung Hwang Seon-Ung Hwang Junchul David Yoon Junchul David Yoon Joohyeong Lee Joohyeong Lee Eunhye Kim Eunhye Kim Lian Cai Lian Cai Gahye Kim Gahye Kim Hyerin Choi Hyerin Choi Dongjin Oh Dongjin Oh Sang-Hwan Hyun Sang-Hwan Hyun Sang-Hwan Hyun |
| author_facet |
Mirae Kim Mirae Kim Seon-Ung Hwang Seon-Ung Hwang Junchul David Yoon Junchul David Yoon Joohyeong Lee Joohyeong Lee Eunhye Kim Eunhye Kim Lian Cai Lian Cai Gahye Kim Gahye Kim Hyerin Choi Hyerin Choi Dongjin Oh Dongjin Oh Sang-Hwan Hyun Sang-Hwan Hyun Sang-Hwan Hyun |
| author_sort |
Mirae Kim |
| title |
Beneficial Effects of Neurotrophin-4 Supplementation During in vitro Maturation of Porcine Cumulus-Oocyte Complexes and Subsequent Embryonic Development After Parthenogenetic Activation |
| title_short |
Beneficial Effects of Neurotrophin-4 Supplementation During in vitro Maturation of Porcine Cumulus-Oocyte Complexes and Subsequent Embryonic Development After Parthenogenetic Activation |
| title_full |
Beneficial Effects of Neurotrophin-4 Supplementation During in vitro Maturation of Porcine Cumulus-Oocyte Complexes and Subsequent Embryonic Development After Parthenogenetic Activation |
| title_fullStr |
Beneficial Effects of Neurotrophin-4 Supplementation During in vitro Maturation of Porcine Cumulus-Oocyte Complexes and Subsequent Embryonic Development After Parthenogenetic Activation |
| title_full_unstemmed |
Beneficial Effects of Neurotrophin-4 Supplementation During in vitro Maturation of Porcine Cumulus-Oocyte Complexes and Subsequent Embryonic Development After Parthenogenetic Activation |
| title_sort |
beneficial effects of neurotrophin-4 supplementation during in vitro maturation of porcine cumulus-oocyte complexes and subsequent embryonic development after parthenogenetic activation |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/6816925bd6534335969a681142e7f445 |
| work_keys_str_mv |
AT miraekim beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation AT miraekim beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation AT seonunghwang beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation AT seonunghwang beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation AT junchuldavidyoon beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation AT junchuldavidyoon beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation AT joohyeonglee beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation AT joohyeonglee beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation AT eunhyekim beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation AT eunhyekim beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation AT liancai beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation AT liancai beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation AT gahyekim beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation AT gahyekim beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation AT hyerinchoi beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation AT hyerinchoi beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation AT dongjinoh beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation AT dongjinoh beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation AT sanghwanhyun beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation AT sanghwanhyun beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation AT sanghwanhyun beneficialeffectsofneurotrophin4supplementationduringinvitromaturationofporcinecumulusoocytecomplexesandsubsequentembryonicdevelopmentafterparthenogeneticactivation |
| _version_ |
1718431174706069504 |