Biologically relevant laminin as chemically defined and fully human platform for human epidermal keratinocyte culture

In vitro expansion of human epidermal keratinocytes to resurface severe wound defects still relies on a human/mouse xenograft culture system. Here the authors develop a fully human, xeno-free culture system using skin-associated laminins, normally present in vivo, to replace mouse feeder cells.

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Auteurs principaux:	Monica Suryana Tjin, Alvin Wen Choong Chua, Aida Moreno-Moral, Li Yen Chong, Po Yin Tang, Nathan Peter Harmston, Zuhua Cai, Enrico Petretto, Bien Keem Tan, Karl Tryggvason
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2018
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/6816bfa2e9c245778f6cdd8f174d79bb
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spelling	oai:doaj.org-article:6816bfa2e9c245778f6cdd8f174d79bb2021-12-02T16:49:50ZBiologically relevant laminin as chemically defined and fully human platform for human epidermal keratinocyte culture10.1038/s41467-018-06934-32041-1723https://doaj.org/article/6816bfa2e9c245778f6cdd8f174d79bb2018-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-06934-3https://doaj.org/toc/2041-1723In vitro expansion of human epidermal keratinocytes to resurface severe wound defects still relies on a human/mouse xenograft culture system. Here the authors develop a fully human, xeno-free culture system using skin-associated laminins, normally present in vivo, to replace mouse feeder cells.Monica Suryana TjinAlvin Wen Choong ChuaAida Moreno-MoralLi Yen ChongPo Yin TangNathan Peter HarmstonZuhua CaiEnrico PetrettoBien Keem TanKarl TryggvasonNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Monica Suryana Tjin Alvin Wen Choong Chua Aida Moreno-Moral Li Yen Chong Po Yin Tang Nathan Peter Harmston Zuhua Cai Enrico Petretto Bien Keem Tan Karl Tryggvason Biologically relevant laminin as chemically defined and fully human platform for human epidermal keratinocyte culture
description	In vitro expansion of human epidermal keratinocytes to resurface severe wound defects still relies on a human/mouse xenograft culture system. Here the authors develop a fully human, xeno-free culture system using skin-associated laminins, normally present in vivo, to replace mouse feeder cells.
format	article
author	Monica Suryana Tjin Alvin Wen Choong Chua Aida Moreno-Moral Li Yen Chong Po Yin Tang Nathan Peter Harmston Zuhua Cai Enrico Petretto Bien Keem Tan Karl Tryggvason
author_facet	Monica Suryana Tjin Alvin Wen Choong Chua Aida Moreno-Moral Li Yen Chong Po Yin Tang Nathan Peter Harmston Zuhua Cai Enrico Petretto Bien Keem Tan Karl Tryggvason
author_sort	Monica Suryana Tjin
title	Biologically relevant laminin as chemically defined and fully human platform for human epidermal keratinocyte culture
title_short	Biologically relevant laminin as chemically defined and fully human platform for human epidermal keratinocyte culture
title_full	Biologically relevant laminin as chemically defined and fully human platform for human epidermal keratinocyte culture
title_fullStr	Biologically relevant laminin as chemically defined and fully human platform for human epidermal keratinocyte culture
title_full_unstemmed	Biologically relevant laminin as chemically defined and fully human platform for human epidermal keratinocyte culture
title_sort	biologically relevant laminin as chemically defined and fully human platform for human epidermal keratinocyte culture
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/6816bfa2e9c245778f6cdd8f174d79bb
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Biologically relevant laminin as chemically defined and fully human platform for human epidermal keratinocyte culture

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