Detecting RNA base methylations in single cells by in situ hybridization

Methylated RNA bases influence many life processes, but current detection methods lack the ability to detect individual methylations in single cells. Here, the authors use fluorescence hybridization probes sensitive to methylation to detect specific epitranscriptomic modifications at the single-cell...

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Autores principales:	Rohan T. Ranasinghe, Martin R. Challand, Kristina A. Ganzinger, Benjamin W. Lewis, Charlotte Softley, Wolfgang H. Schmied, Mathew H. Horrocks, Nadia Shivji, Jason W. Chin, James Spencer, David Klenerman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/681a36aec6e7448383e2cd2cf34e6586
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spelling	oai:doaj.org-article:681a36aec6e7448383e2cd2cf34e65862021-12-02T15:34:19ZDetecting RNA base methylations in single cells by in situ hybridization10.1038/s41467-017-02714-72041-1723https://doaj.org/article/681a36aec6e7448383e2cd2cf34e65862018-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-02714-7https://doaj.org/toc/2041-1723Methylated RNA bases influence many life processes, but current detection methods lack the ability to detect individual methylations in single cells. Here, the authors use fluorescence hybridization probes sensitive to methylation to detect specific epitranscriptomic modifications at the single-cell level.Rohan T. RanasingheMartin R. ChallandKristina A. GanzingerBenjamin W. LewisCharlotte SoftleyWolfgang H. SchmiedMathew H. HorrocksNadia ShivjiJason W. ChinJames SpencerDavid KlenermanNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Rohan T. Ranasinghe Martin R. Challand Kristina A. Ganzinger Benjamin W. Lewis Charlotte Softley Wolfgang H. Schmied Mathew H. Horrocks Nadia Shivji Jason W. Chin James Spencer David Klenerman Detecting RNA base methylations in single cells by in situ hybridization
description	Methylated RNA bases influence many life processes, but current detection methods lack the ability to detect individual methylations in single cells. Here, the authors use fluorescence hybridization probes sensitive to methylation to detect specific epitranscriptomic modifications at the single-cell level.
format	article
author	Rohan T. Ranasinghe Martin R. Challand Kristina A. Ganzinger Benjamin W. Lewis Charlotte Softley Wolfgang H. Schmied Mathew H. Horrocks Nadia Shivji Jason W. Chin James Spencer David Klenerman
author_facet	Rohan T. Ranasinghe Martin R. Challand Kristina A. Ganzinger Benjamin W. Lewis Charlotte Softley Wolfgang H. Schmied Mathew H. Horrocks Nadia Shivji Jason W. Chin James Spencer David Klenerman
author_sort	Rohan T. Ranasinghe
title	Detecting RNA base methylations in single cells by in situ hybridization
title_short	Detecting RNA base methylations in single cells by in situ hybridization
title_full	Detecting RNA base methylations in single cells by in situ hybridization
title_fullStr	Detecting RNA base methylations in single cells by in situ hybridization
title_full_unstemmed	Detecting RNA base methylations in single cells by in situ hybridization
title_sort	detecting rna base methylations in single cells by in situ hybridization
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/681a36aec6e7448383e2cd2cf34e6586
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Detecting RNA base methylations in single cells by in situ hybridization

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