Development of a nondiapausing strain of northern corn rootworm with rearing techniques for both diapausing and nondiapausing strains

Abstract The northern corn rootworm, Diabrotica barberi Smith & Lawrence, has a univoltine life cycle that typically produces one generation a year. When rearing the northern corn rootworm in the laboratory, in order to break diapause, it is necessary to expose eggs to a five month cold period b...

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Autores principales:	Man P. Huynh, Chad Nielson, B. Wade French, Dalton C. Ludwick, Ryan W. Geisert, Adriano E. Pereira, Julie Barry, Lisa N. Meihls, Sharon K. Schneider, Bruce E. Hibbard
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/681bc5f2a95c406681f44fb6b556176a
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spelling	oai:doaj.org-article:681bc5f2a95c406681f44fb6b556176a2021-12-02T14:58:48ZDevelopment of a nondiapausing strain of northern corn rootworm with rearing techniques for both diapausing and nondiapausing strains10.1038/s41598-021-97452-82045-2322https://doaj.org/article/681bc5f2a95c406681f44fb6b556176a2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97452-8https://doaj.org/toc/2045-2322Abstract The northern corn rootworm, Diabrotica barberi Smith & Lawrence, has a univoltine life cycle that typically produces one generation a year. When rearing the northern corn rootworm in the laboratory, in order to break diapause, it is necessary to expose eggs to a five month cold period before raising the temperature. By selective breeding of the small fraction of eggs that hatched without cold within 19–32 days post oviposition, we were able to develop a non-diapausing colony of the northern corn rootworm within five generations of selection. Through selection, the percentages of adult emergence from egg hatch without exposure to cold treatment significantly increased from 0.52% ± 0.07 at generation zero to 29.0% ± 2.47 at generation eight. During this process, we developed an improved method for laboratory rearing of both the newly developed non-diapausing strain as well as the diapausing strain. The development of the non-diapausing colony along with the improvements to the rearing system will allow researchers to produce up to six generations of the northern corn rootworm per year, which would facilitate research and advance our knowledge of this pest at an accelerated rate.Man P. HuynhChad NielsonB. Wade FrenchDalton C. LudwickRyan W. GeisertAdriano E. PereiraJulie BarryLisa N. MeihlsSharon K. SchneiderBruce E. HibbardNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Man P. Huynh Chad Nielson B. Wade French Dalton C. Ludwick Ryan W. Geisert Adriano E. Pereira Julie Barry Lisa N. Meihls Sharon K. Schneider Bruce E. Hibbard Development of a nondiapausing strain of northern corn rootworm with rearing techniques for both diapausing and nondiapausing strains
description	Abstract The northern corn rootworm, Diabrotica barberi Smith & Lawrence, has a univoltine life cycle that typically produces one generation a year. When rearing the northern corn rootworm in the laboratory, in order to break diapause, it is necessary to expose eggs to a five month cold period before raising the temperature. By selective breeding of the small fraction of eggs that hatched without cold within 19–32 days post oviposition, we were able to develop a non-diapausing colony of the northern corn rootworm within five generations of selection. Through selection, the percentages of adult emergence from egg hatch without exposure to cold treatment significantly increased from 0.52% ± 0.07 at generation zero to 29.0% ± 2.47 at generation eight. During this process, we developed an improved method for laboratory rearing of both the newly developed non-diapausing strain as well as the diapausing strain. The development of the non-diapausing colony along with the improvements to the rearing system will allow researchers to produce up to six generations of the northern corn rootworm per year, which would facilitate research and advance our knowledge of this pest at an accelerated rate.
format	article
author	Man P. Huynh Chad Nielson B. Wade French Dalton C. Ludwick Ryan W. Geisert Adriano E. Pereira Julie Barry Lisa N. Meihls Sharon K. Schneider Bruce E. Hibbard
author_facet	Man P. Huynh Chad Nielson B. Wade French Dalton C. Ludwick Ryan W. Geisert Adriano E. Pereira Julie Barry Lisa N. Meihls Sharon K. Schneider Bruce E. Hibbard
author_sort	Man P. Huynh
title	Development of a nondiapausing strain of northern corn rootworm with rearing techniques for both diapausing and nondiapausing strains
title_short	Development of a nondiapausing strain of northern corn rootworm with rearing techniques for both diapausing and nondiapausing strains
title_full	Development of a nondiapausing strain of northern corn rootworm with rearing techniques for both diapausing and nondiapausing strains
title_fullStr	Development of a nondiapausing strain of northern corn rootworm with rearing techniques for both diapausing and nondiapausing strains
title_full_unstemmed	Development of a nondiapausing strain of northern corn rootworm with rearing techniques for both diapausing and nondiapausing strains
title_sort	development of a nondiapausing strain of northern corn rootworm with rearing techniques for both diapausing and nondiapausing strains
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/681bc5f2a95c406681f44fb6b556176a
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Development of a nondiapausing strain of northern corn rootworm with rearing techniques for both diapausing and nondiapausing strains

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