RNA-seq analysis reveals different dynamics of differentiation of human dermis- and adipose-derived stromal stem cells.

<h4>Background</h4>Tissue regeneration and recovery in the adult body depends on self-renewal and differentiation of stem and progenitor cells. Mesenchymal stem cells (MSCs) that have the ability to differentiate into various cell types, have been isolated from the stromal fraction of vi...

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Autores principales:	Kersti Jääger, Saiful Islam, Pawel Zajac, Sten Linnarsson, Toomas Neuman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6824e2cec62242578b2254cea68273db
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spelling	oai:doaj.org-article:6824e2cec62242578b2254cea68273db2021-11-18T07:15:01ZRNA-seq analysis reveals different dynamics of differentiation of human dermis- and adipose-derived stromal stem cells.1932-620310.1371/journal.pone.0038833https://doaj.org/article/6824e2cec62242578b2254cea68273db2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22723894/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Tissue regeneration and recovery in the adult body depends on self-renewal and differentiation of stem and progenitor cells. Mesenchymal stem cells (MSCs) that have the ability to differentiate into various cell types, have been isolated from the stromal fraction of virtually all tissues. However, little is known about the true identity of MSCs. MSC populations exhibit great tissue-, location- and patient-specific variation in gene expression and are heterogeneous in cell composition.<h4>Methodology/principal findings</h4>Our aim was to analyze the dynamics of differentiation of two closely related stromal cell types, adipose tissue-derived MSCs (AdMSCs) and dermal fibroblasts (FBs) along adipogenic, osteogenic and chondrogenic lineages using multiplex RNA-seq technology. We found that undifferentiated donor-matched AdMSCs and FBs are distinct populations that stay different upon differentiation into adipocytes, osteoblasts and chondrocytes. The changes in lineage-specific gene expression occur early in differentiation and persist over time in both AdMSCs and FBs. Further, AdMSCs and FBs exhibit similar dynamics of adipogenic and osteogenic differentiation but different dynamics of chondrogenic differentiation.<h4>Conclusions/significance</h4>Our findings suggest that stromal stem cells including AdMSCs and dermal FBs exploit different molecular mechanisms of differentiation to reach a common cell fate. The early mechanisms of differentiation are lineage-specific and are similar for adipogenic and osteogenic differentiation but are distinct for chondrogenic differentiation between AdMSCs and FBs.Kersti JäägerSaiful IslamPawel ZajacSten LinnarssonToomas NeumanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e38833 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kersti Jääger Saiful Islam Pawel Zajac Sten Linnarsson Toomas Neuman RNA-seq analysis reveals different dynamics of differentiation of human dermis- and adipose-derived stromal stem cells.
description	<h4>Background</h4>Tissue regeneration and recovery in the adult body depends on self-renewal and differentiation of stem and progenitor cells. Mesenchymal stem cells (MSCs) that have the ability to differentiate into various cell types, have been isolated from the stromal fraction of virtually all tissues. However, little is known about the true identity of MSCs. MSC populations exhibit great tissue-, location- and patient-specific variation in gene expression and are heterogeneous in cell composition.<h4>Methodology/principal findings</h4>Our aim was to analyze the dynamics of differentiation of two closely related stromal cell types, adipose tissue-derived MSCs (AdMSCs) and dermal fibroblasts (FBs) along adipogenic, osteogenic and chondrogenic lineages using multiplex RNA-seq technology. We found that undifferentiated donor-matched AdMSCs and FBs are distinct populations that stay different upon differentiation into adipocytes, osteoblasts and chondrocytes. The changes in lineage-specific gene expression occur early in differentiation and persist over time in both AdMSCs and FBs. Further, AdMSCs and FBs exhibit similar dynamics of adipogenic and osteogenic differentiation but different dynamics of chondrogenic differentiation.<h4>Conclusions/significance</h4>Our findings suggest that stromal stem cells including AdMSCs and dermal FBs exploit different molecular mechanisms of differentiation to reach a common cell fate. The early mechanisms of differentiation are lineage-specific and are similar for adipogenic and osteogenic differentiation but are distinct for chondrogenic differentiation between AdMSCs and FBs.
format	article
author	Kersti Jääger Saiful Islam Pawel Zajac Sten Linnarsson Toomas Neuman
author_facet	Kersti Jääger Saiful Islam Pawel Zajac Sten Linnarsson Toomas Neuman
author_sort	Kersti Jääger
title	RNA-seq analysis reveals different dynamics of differentiation of human dermis- and adipose-derived stromal stem cells.
title_short	RNA-seq analysis reveals different dynamics of differentiation of human dermis- and adipose-derived stromal stem cells.
title_full	RNA-seq analysis reveals different dynamics of differentiation of human dermis- and adipose-derived stromal stem cells.
title_fullStr	RNA-seq analysis reveals different dynamics of differentiation of human dermis- and adipose-derived stromal stem cells.
title_full_unstemmed	RNA-seq analysis reveals different dynamics of differentiation of human dermis- and adipose-derived stromal stem cells.
title_sort	rna-seq analysis reveals different dynamics of differentiation of human dermis- and adipose-derived stromal stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/6824e2cec62242578b2254cea68273db
work_keys_str_mv	AT kerstijaager rnaseqanalysisrevealsdifferentdynamicsofdifferentiationofhumandermisandadiposederivedstromalstemcells AT saifulislam rnaseqanalysisrevealsdifferentdynamicsofdifferentiationofhumandermisandadiposederivedstromalstemcells AT pawelzajac rnaseqanalysisrevealsdifferentdynamicsofdifferentiationofhumandermisandadiposederivedstromalstemcells AT stenlinnarsson rnaseqanalysisrevealsdifferentdynamicsofdifferentiationofhumandermisandadiposederivedstromalstemcells AT toomasneuman rnaseqanalysisrevealsdifferentdynamicsofdifferentiationofhumandermisandadiposederivedstromalstemcells
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RNA-seq analysis reveals different dynamics of differentiation of human dermis- and adipose-derived stromal stem cells.

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