Infection Process and Genome Assembly Provide Insights into the Pathogenic Mechanism of Destructive Mycoparasite Calcarisporium cordycipiticola with Host Specificity

Calcarisporium cordycipiticola is the pathogen in the white mildew disease of Cordyceps militaris, one of the popular mushrooms. This disease frequently occurs and there is no effective method for disease prevention and control. In the present study, C....

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Autores principales:	Qing Liu, Yanyan Xu, Xiaoling Zhang, Kuan Li, Xiao Li, Fen Wang, Fangxu Xu, Caihong Dong
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Calcarisporium cordycipiticola</i> <i>Cordyceps militaris</i> white mildew disease infection process genome sequencing host specificity Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/6830514c5a284c0e86596204929a357b
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spelling	oai:doaj.org-article:6830514c5a284c0e86596204929a357b2021-11-25T18:05:43ZInfection Process and Genome Assembly Provide Insights into the Pathogenic Mechanism of Destructive Mycoparasite <i>Calcarisporium cordycipiticola</i> with Host Specificity10.3390/jof71109182309-608Xhttps://doaj.org/article/6830514c5a284c0e86596204929a357b2021-10-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/918https://doaj.org/toc/2309-608X<i>Calcarisporium cordycipiticola</i> is the pathogen in the white mildew disease of <i>Cordyceps militaris</i>, one of the popular mushrooms. This disease frequently occurs and there is no effective method for disease prevention and control. In the present study, <i>C. militaris</i> is found to be the only host of <i>C. cordycipiticola</i>, indicating strict host specificity. The infection process was monitored by fluorescent labeling and scanning and transmission electron microscopes. <i>C. cordycipiticola</i> can invade into the gaps among hyphae of the fruiting bodies of the host and fill them gradually. It can degrade the hyphae of the host by both direct contact and noncontact. The parasitism is initially biotrophic, and then necrotrophic as mycoparasitic interaction progresses. The approximate chromosome-level genome assembly of <i>C. cordycipiticola</i> yielded an N50 length of 5.45 Mbp and a total size of 34.51 Mbp, encoding 10,443 proteins. Phylogenomic analysis revealed that <i>C. cordycipiticola</i> is phylogenetically close to its specific host, <i>C. militaris.</i> A comparative genomic analysis showed that the number of CAZymes of <i>C. cordycipiticola</i> was much less than in other mycoparasites, which might be attributed to its host specificity. Secondary metabolite cluster analysis disclosed the great biosynthetic capabilities and potential mycotoxin production capability. This study provides insights into the potential pathogenesis and interaction between mycoparasite and its host.Qing LiuYanyan XuXiaoling ZhangKuan LiXiao LiFen WangFangxu XuCaihong DongMDPI AGarticle<i>Calcarisporium cordycipiticola</i><i>Cordyceps militaris</i>white mildew diseaseinfection processgenome sequencinghost specificityBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 918, p 918 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Calcarisporium cordycipiticola</i> <i>Cordyceps militaris</i> white mildew disease infection process genome sequencing host specificity Biology (General) QH301-705.5
spellingShingle	<i>Calcarisporium cordycipiticola</i> <i>Cordyceps militaris</i> white mildew disease infection process genome sequencing host specificity Biology (General) QH301-705.5 Qing Liu Yanyan Xu Xiaoling Zhang Kuan Li Xiao Li Fen Wang Fangxu Xu Caihong Dong Infection Process and Genome Assembly Provide Insights into the Pathogenic Mechanism of Destructive Mycoparasite <i>Calcarisporium cordycipiticola</i> with Host Specificity
description	<i>Calcarisporium cordycipiticola</i> is the pathogen in the white mildew disease of <i>Cordyceps militaris</i>, one of the popular mushrooms. This disease frequently occurs and there is no effective method for disease prevention and control. In the present study, <i>C. militaris</i> is found to be the only host of <i>C. cordycipiticola</i>, indicating strict host specificity. The infection process was monitored by fluorescent labeling and scanning and transmission electron microscopes. <i>C. cordycipiticola</i> can invade into the gaps among hyphae of the fruiting bodies of the host and fill them gradually. It can degrade the hyphae of the host by both direct contact and noncontact. The parasitism is initially biotrophic, and then necrotrophic as mycoparasitic interaction progresses. The approximate chromosome-level genome assembly of <i>C. cordycipiticola</i> yielded an N50 length of 5.45 Mbp and a total size of 34.51 Mbp, encoding 10,443 proteins. Phylogenomic analysis revealed that <i>C. cordycipiticola</i> is phylogenetically close to its specific host, <i>C. militaris.</i> A comparative genomic analysis showed that the number of CAZymes of <i>C. cordycipiticola</i> was much less than in other mycoparasites, which might be attributed to its host specificity. Secondary metabolite cluster analysis disclosed the great biosynthetic capabilities and potential mycotoxin production capability. This study provides insights into the potential pathogenesis and interaction between mycoparasite and its host.
format	article
author	Qing Liu Yanyan Xu Xiaoling Zhang Kuan Li Xiao Li Fen Wang Fangxu Xu Caihong Dong
author_facet	Qing Liu Yanyan Xu Xiaoling Zhang Kuan Li Xiao Li Fen Wang Fangxu Xu Caihong Dong
author_sort	Qing Liu
title	Infection Process and Genome Assembly Provide Insights into the Pathogenic Mechanism of Destructive Mycoparasite <i>Calcarisporium cordycipiticola</i> with Host Specificity
title_short	Infection Process and Genome Assembly Provide Insights into the Pathogenic Mechanism of Destructive Mycoparasite <i>Calcarisporium cordycipiticola</i> with Host Specificity
title_full	Infection Process and Genome Assembly Provide Insights into the Pathogenic Mechanism of Destructive Mycoparasite <i>Calcarisporium cordycipiticola</i> with Host Specificity
title_fullStr	Infection Process and Genome Assembly Provide Insights into the Pathogenic Mechanism of Destructive Mycoparasite <i>Calcarisporium cordycipiticola</i> with Host Specificity
title_full_unstemmed	Infection Process and Genome Assembly Provide Insights into the Pathogenic Mechanism of Destructive Mycoparasite <i>Calcarisporium cordycipiticola</i> with Host Specificity
title_sort	infection process and genome assembly provide insights into the pathogenic mechanism of destructive mycoparasite <i>calcarisporium cordycipiticola</i> with host specificity
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6830514c5a284c0e86596204929a357b
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Infection Process and Genome Assembly Provide Insights into the Pathogenic Mechanism of Destructive Mycoparasite <i>Calcarisporium cordycipiticola</i> with Host Specificity

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