Formulation of nanotized curcumin and demonstration of its antimalarial efficacy

Aparajita Ghosh,1 Tanushree Banerjee,2 Suman Bhandary,1 Avadhesha Surolia31Division of Molecular Medicine, Bose Institute, Centenary Campus, Kolkata, West Bengal, India; 2Department of Biotechnology, University of Pune, Pune, India; 3Molecular Biophysics Unit, Indian Institute of Science, Bangalore...

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Autores principales:	Ghosh A, Banerjee T, Bhandary S, Surolia A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6833c7c3a184427aaf056bcb58777df3
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spelling	oai:doaj.org-article:6833c7c3a184427aaf056bcb58777df32021-12-02T02:43:41ZFormulation of nanotized curcumin and demonstration of its antimalarial efficacy1178-2013https://doaj.org/article/6833c7c3a184427aaf056bcb58777df32014-11-01T00:00:00Zhttp://www.dovepress.com/formulation-of-nanotized-curcumin-and-demonstration-of-its-antimalaria-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Aparajita Ghosh,1 Tanushree Banerjee,2 Suman Bhandary,1 Avadhesha Surolia31Division of Molecular Medicine, Bose Institute, Centenary Campus, Kolkata, West Bengal, India; 2Department of Biotechnology, University of Pune, Pune, India; 3Molecular Biophysics Unit, Indian Institute of Science, Bangalore, IndiaAim: The present study was conducted to overcome the disadvantages associated with the poor water solubility and low bioavailability of curcumin by synthesizing nanotized curcumin and demonstrating its efficacy in treating malaria. Materials and methods: Nanotized curcumin was prepared by a modified emulsion-diffusion-evaporation method and was characterized by means of transmission electron microscopy, atomic force microscopy, dynamic light scattering, Zetasizer, Fourier transform infrared spectroscopy, and differential thermal analysis. The novelty of the prepared nanoformulation lies in the fact that it was devoid of any polymeric matrices used in conventional carriers. The antimalarial efficacy of the prepared nanotized curcumin was then checked both in vitro and in vivo. Results: The nanopreparation was found to be non-toxic and had a particle size distribution of 20–50 nm along with improved aqueous dispersibility and an entrapment efficiency of 45%. Nanotized curcumin (half maximal inhibitory concentration [IC50]: 0.5 µM) was also found to be ten-fold more effective for growth inhibition of Plasmodium falciparum in vitro as compared to its native counterpart (IC50: 5 µM). Oral bioavailability of nanotized curcumin was found to be superior to that of its native counterpart. Moreover, when Plasmodium berghei-infected mice were orally treated with nanotized curcumin, it prolonged their survival by more than 2 months with complete clearance of parasites in comparison to the untreated animals, which survived for 8 days only. Conclusion: Nanotized curcumin holds a considerable promise in therapeutics as demonstrated here for treating malaria as a test system. Keywords: curcumin, nanotized curcumin, P. berghei, P. falciparum, antimalarialGhosh ABanerjee TBhandary SSurolia ADove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 5373-5387 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ghosh A Banerjee T Bhandary S Surolia A Formulation of nanotized curcumin and demonstration of its antimalarial efficacy
description	Aparajita Ghosh,1 Tanushree Banerjee,2 Suman Bhandary,1 Avadhesha Surolia31Division of Molecular Medicine, Bose Institute, Centenary Campus, Kolkata, West Bengal, India; 2Department of Biotechnology, University of Pune, Pune, India; 3Molecular Biophysics Unit, Indian Institute of Science, Bangalore, IndiaAim: The present study was conducted to overcome the disadvantages associated with the poor water solubility and low bioavailability of curcumin by synthesizing nanotized curcumin and demonstrating its efficacy in treating malaria. Materials and methods: Nanotized curcumin was prepared by a modified emulsion-diffusion-evaporation method and was characterized by means of transmission electron microscopy, atomic force microscopy, dynamic light scattering, Zetasizer, Fourier transform infrared spectroscopy, and differential thermal analysis. The novelty of the prepared nanoformulation lies in the fact that it was devoid of any polymeric matrices used in conventional carriers. The antimalarial efficacy of the prepared nanotized curcumin was then checked both in vitro and in vivo. Results: The nanopreparation was found to be non-toxic and had a particle size distribution of 20–50 nm along with improved aqueous dispersibility and an entrapment efficiency of 45%. Nanotized curcumin (half maximal inhibitory concentration [IC50]: 0.5 µM) was also found to be ten-fold more effective for growth inhibition of Plasmodium falciparum in vitro as compared to its native counterpart (IC50: 5 µM). Oral bioavailability of nanotized curcumin was found to be superior to that of its native counterpart. Moreover, when Plasmodium berghei-infected mice were orally treated with nanotized curcumin, it prolonged their survival by more than 2 months with complete clearance of parasites in comparison to the untreated animals, which survived for 8 days only. Conclusion: Nanotized curcumin holds a considerable promise in therapeutics as demonstrated here for treating malaria as a test system. Keywords: curcumin, nanotized curcumin, P. berghei, P. falciparum, antimalarial
format	article
author	Ghosh A Banerjee T Bhandary S Surolia A
author_facet	Ghosh A Banerjee T Bhandary S Surolia A
author_sort	Ghosh A
title	Formulation of nanotized curcumin and demonstration of its antimalarial efficacy
title_short	Formulation of nanotized curcumin and demonstration of its antimalarial efficacy
title_full	Formulation of nanotized curcumin and demonstration of its antimalarial efficacy
title_fullStr	Formulation of nanotized curcumin and demonstration of its antimalarial efficacy
title_full_unstemmed	Formulation of nanotized curcumin and demonstration of its antimalarial efficacy
title_sort	formulation of nanotized curcumin and demonstration of its antimalarial efficacy
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/6833c7c3a184427aaf056bcb58777df3
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Formulation of nanotized curcumin and demonstration of its antimalarial efficacy

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