Effect of Ephedrae Herba methanol extract on high-fat diet-induced hyperlipidaemic mice

Context: Ephedrae Herba (EH), the dried stems and leaves of Ephedra sinica Stapf., E. intermedia Schrenk et C. A. Mey., or E. equisetina Bge. (Ephedraceae [Ephedra]) is used to treat respiratory diseases. Recently, especially in the Republic of Korea, EH has also been used for weight reduction. Obje...

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Autores principales:	Se-Eun Lee, Chiyeon Lim, Sehyun Lim, Byoungho Lee, Suin Cho
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	obesity cardiovascular diseases weight reduction herbal medicine Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/683563c9fce24bd69f7962a3d94b6454
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spelling	oai:doaj.org-article:683563c9fce24bd69f7962a3d94b64542021-11-17T14:21:56ZEffect of Ephedrae Herba methanol extract on high-fat diet-induced hyperlipidaemic mice1388-02091744-511610.1080/13880209.2019.1666883https://doaj.org/article/683563c9fce24bd69f7962a3d94b64542019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1666883https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: Ephedrae Herba (EH), the dried stems and leaves of Ephedra sinica Stapf., E. intermedia Schrenk et C. A. Mey., or E. equisetina Bge. (Ephedraceae [Ephedra]) is used to treat respiratory diseases. Recently, especially in the Republic of Korea, EH has also been used for weight reduction. Objective: We evaluated the effects and molecular targets of methanol EH extract (EHM) on high-fat diet (HFD)-induced hyperlipidemic ICR mice. Materials and methods: EHM was orally administered (100 mg/kg body weight/day) for 3 weeks. We observed changes in body weight (BW), total cholesterol (TC), high-density lipoprotein–cholesterol, and triglycerides to evaluate the physiological changes induced by HFD or EHM administration. To evaluate lipid peroxidation and liver toxicity, malondialdehyde and blood alanine aminotransferase levels were measured. In addition to analyzing liver gene expression profiles, EHM target proteins were identified using a protein interaction database. Results: EHM administration for 3 weeks significantly (p < 0.05) decreased TC and triglyceride levels without altering BW in mice, and gene expression levels in the livers of EHM-treated mice were restored at 34.0% and 48.4% of those up- or down-regulated by hyperlipidaemia, respectively. Proteins related to DNA repair and energy metabolism were identified via protein interaction network analysis as molecular targets of EHM that play key roles in ameliorating hyperlipidaemia. Discussion and conclusions: EHM regulated hyperlipidaemia by decreasing total blood lipid and triglyceride levels in hyperlipidaemic mice. EHM showed preventive effects against hyperlipidaemia in mice, possibly via the regulation of DNA repair and the expression of energy metabolism-related genes and proteins.Se-Eun LeeChiyeon LimSehyun LimByoungho LeeSuin ChoTaylor & Francis Grouparticleobesitycardiovascular diseasesweight reductionherbal medicineTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 676-683 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	obesity cardiovascular diseases weight reduction herbal medicine Therapeutics. Pharmacology RM1-950
spellingShingle	obesity cardiovascular diseases weight reduction herbal medicine Therapeutics. Pharmacology RM1-950 Se-Eun Lee Chiyeon Lim Sehyun Lim Byoungho Lee Suin Cho Effect of Ephedrae Herba methanol extract on high-fat diet-induced hyperlipidaemic mice
description	Context: Ephedrae Herba (EH), the dried stems and leaves of Ephedra sinica Stapf., E. intermedia Schrenk et C. A. Mey., or E. equisetina Bge. (Ephedraceae [Ephedra]) is used to treat respiratory diseases. Recently, especially in the Republic of Korea, EH has also been used for weight reduction. Objective: We evaluated the effects and molecular targets of methanol EH extract (EHM) on high-fat diet (HFD)-induced hyperlipidemic ICR mice. Materials and methods: EHM was orally administered (100 mg/kg body weight/day) for 3 weeks. We observed changes in body weight (BW), total cholesterol (TC), high-density lipoprotein–cholesterol, and triglycerides to evaluate the physiological changes induced by HFD or EHM administration. To evaluate lipid peroxidation and liver toxicity, malondialdehyde and blood alanine aminotransferase levels were measured. In addition to analyzing liver gene expression profiles, EHM target proteins were identified using a protein interaction database. Results: EHM administration for 3 weeks significantly (p < 0.05) decreased TC and triglyceride levels without altering BW in mice, and gene expression levels in the livers of EHM-treated mice were restored at 34.0% and 48.4% of those up- or down-regulated by hyperlipidaemia, respectively. Proteins related to DNA repair and energy metabolism were identified via protein interaction network analysis as molecular targets of EHM that play key roles in ameliorating hyperlipidaemia. Discussion and conclusions: EHM regulated hyperlipidaemia by decreasing total blood lipid and triglyceride levels in hyperlipidaemic mice. EHM showed preventive effects against hyperlipidaemia in mice, possibly via the regulation of DNA repair and the expression of energy metabolism-related genes and proteins.
format	article
author	Se-Eun Lee Chiyeon Lim Sehyun Lim Byoungho Lee Suin Cho
author_facet	Se-Eun Lee Chiyeon Lim Sehyun Lim Byoungho Lee Suin Cho
author_sort	Se-Eun Lee
title	Effect of Ephedrae Herba methanol extract on high-fat diet-induced hyperlipidaemic mice
title_short	Effect of Ephedrae Herba methanol extract on high-fat diet-induced hyperlipidaemic mice
title_full	Effect of Ephedrae Herba methanol extract on high-fat diet-induced hyperlipidaemic mice
title_fullStr	Effect of Ephedrae Herba methanol extract on high-fat diet-induced hyperlipidaemic mice
title_full_unstemmed	Effect of Ephedrae Herba methanol extract on high-fat diet-induced hyperlipidaemic mice
title_sort	effect of ephedrae herba methanol extract on high-fat diet-induced hyperlipidaemic mice
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/683563c9fce24bd69f7962a3d94b6454
work_keys_str_mv	AT seeunlee effectofephedraeherbamethanolextractonhighfatdietinducedhyperlipidaemicmice AT chiyeonlim effectofephedraeherbamethanolextractonhighfatdietinducedhyperlipidaemicmice AT sehyunlim effectofephedraeherbamethanolextractonhighfatdietinducedhyperlipidaemicmice AT byoungholee effectofephedraeherbamethanolextractonhighfatdietinducedhyperlipidaemicmice AT suincho effectofephedraeherbamethanolextractonhighfatdietinducedhyperlipidaemicmice
_version_	1718425487512961024

Effect of Ephedrae Herba methanol extract on high-fat diet-induced hyperlipidaemic mice

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