The buckling instability of aggregating red blood cells

Abstract Plasma proteins such as fibrinogen induce the aggregation of red blood cells (RBC) into rouleaux, which are responsible for the pronounced shear thinning behavior of blood, control the erythrocyte sedimentation rate (ESR) – a common hematological test – and are involved in many situations o...

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Autores principales:	Daniel Flormann, Othmane Aouane, Lars Kaestner, Christian Ruloff, Chaouqi Misbah, Thomas Podgorski, Christian Wagner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6837584a5b8b4ceab073024148c64a38
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spelling	oai:doaj.org-article:6837584a5b8b4ceab073024148c64a382021-12-02T16:06:16ZThe buckling instability of aggregating red blood cells10.1038/s41598-017-07634-62045-2322https://doaj.org/article/6837584a5b8b4ceab073024148c64a382017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07634-6https://doaj.org/toc/2045-2322Abstract Plasma proteins such as fibrinogen induce the aggregation of red blood cells (RBC) into rouleaux, which are responsible for the pronounced shear thinning behavior of blood, control the erythrocyte sedimentation rate (ESR) – a common hematological test – and are involved in many situations of physiological relevance such as structuration of blood in the microcirculation or clot formation in pathological situations. Confocal microscopy is used to characterize the shape of RBCs within rouleaux at equilibrium as a function of macromolecular concentration, revealing the diversity of contact zone morphology. Three different configurations that have only been partly predicted before are identified, namely parachute, male-female and sigmoid shapes, and quantitatively recovered by numerical simulations. A detailed experimental and theoretical analysis of clusters of two cells shows that the deformation increases nonlinearly with the interaction energy. Models indicate a forward bifurcation in which the contacting membrane undergoes a buckling instability from a flat to a deformed contact zone at a critical value of the interaction energy. These results are not only relevant for the understanding of the morphology and stability of RBC aggregates, but also for a whole class of interacting soft deformable objects such as vesicles, capsules or cells in tissues.Daniel FlormannOthmane AouaneLars KaestnerChristian RuloffChaouqi MisbahThomas PodgorskiChristian WagnerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel Flormann Othmane Aouane Lars Kaestner Christian Ruloff Chaouqi Misbah Thomas Podgorski Christian Wagner The buckling instability of aggregating red blood cells
description	Abstract Plasma proteins such as fibrinogen induce the aggregation of red blood cells (RBC) into rouleaux, which are responsible for the pronounced shear thinning behavior of blood, control the erythrocyte sedimentation rate (ESR) – a common hematological test – and are involved in many situations of physiological relevance such as structuration of blood in the microcirculation or clot formation in pathological situations. Confocal microscopy is used to characterize the shape of RBCs within rouleaux at equilibrium as a function of macromolecular concentration, revealing the diversity of contact zone morphology. Three different configurations that have only been partly predicted before are identified, namely parachute, male-female and sigmoid shapes, and quantitatively recovered by numerical simulations. A detailed experimental and theoretical analysis of clusters of two cells shows that the deformation increases nonlinearly with the interaction energy. Models indicate a forward bifurcation in which the contacting membrane undergoes a buckling instability from a flat to a deformed contact zone at a critical value of the interaction energy. These results are not only relevant for the understanding of the morphology and stability of RBC aggregates, but also for a whole class of interacting soft deformable objects such as vesicles, capsules or cells in tissues.
format	article
author	Daniel Flormann Othmane Aouane Lars Kaestner Christian Ruloff Chaouqi Misbah Thomas Podgorski Christian Wagner
author_facet	Daniel Flormann Othmane Aouane Lars Kaestner Christian Ruloff Chaouqi Misbah Thomas Podgorski Christian Wagner
author_sort	Daniel Flormann
title	The buckling instability of aggregating red blood cells
title_short	The buckling instability of aggregating red blood cells
title_full	The buckling instability of aggregating red blood cells
title_fullStr	The buckling instability of aggregating red blood cells
title_full_unstemmed	The buckling instability of aggregating red blood cells
title_sort	buckling instability of aggregating red blood cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6837584a5b8b4ceab073024148c64a38
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The buckling instability of aggregating red blood cells

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