Progress of Microfluidic Continuous Separation Techniques for Micro-/Nanoscale Bioparticles

Separation of micro- and nano-sized biological particles, such as cells, proteins, and nucleotides, is at the heart of most biochemical sensing/analysis, including in vitro biosensing, diagnostics, drug development, proteomics, and genomics. However, most of the conventional particle separation tech...

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Autores principales:	Se-woon Choe, Bumjoo Kim, Minseok Kim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	microfluidics separation bioparticles biosensors biosample preparation Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/683ae55591fa4056b32fe798900bd68d
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spelling	oai:doaj.org-article:683ae55591fa4056b32fe798900bd68d2021-11-25T16:55:48ZProgress of Microfluidic Continuous Separation Techniques for Micro-/Nanoscale Bioparticles10.3390/bios111104642079-6374https://doaj.org/article/683ae55591fa4056b32fe798900bd68d2021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6374/11/11/464https://doaj.org/toc/2079-6374Separation of micro- and nano-sized biological particles, such as cells, proteins, and nucleotides, is at the heart of most biochemical sensing/analysis, including in vitro biosensing, diagnostics, drug development, proteomics, and genomics. However, most of the conventional particle separation techniques are based on membrane filtration techniques, whose efficiency is limited by membrane characteristics, such as pore size, porosity, surface charge density, or biocompatibility, which results in a reduction in the separation efficiency of bioparticles of various sizes and types. In addition, since other conventional separation methods, such as centrifugation, chromatography, and precipitation, are difficult to perform in a continuous manner, requiring multiple preparation steps with a relatively large minimum sample volume is necessary for stable bioprocessing. Recently, microfluidic engineering enables more efficient separation in a continuous flow with rapid processing of small volumes of rare biological samples, such as DNA, proteins, viruses, exosomes, and even cells. In this paper, we present a comprehensive review of the recent advances in microfluidic separation of micro-/nano-sized bioparticles by summarizing the physical principles behind the separation system and practical examples of biomedical applications.Se-woon ChoeBumjoo KimMinseok KimMDPI AGarticlemicrofluidicsseparationbioparticlesbiosensorsbiosample preparationBiotechnologyTP248.13-248.65ENBiosensors, Vol 11, Iss 464, p 464 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microfluidics separation bioparticles biosensors biosample preparation Biotechnology TP248.13-248.65
spellingShingle	microfluidics separation bioparticles biosensors biosample preparation Biotechnology TP248.13-248.65 Se-woon Choe Bumjoo Kim Minseok Kim Progress of Microfluidic Continuous Separation Techniques for Micro-/Nanoscale Bioparticles
description	Separation of micro- and nano-sized biological particles, such as cells, proteins, and nucleotides, is at the heart of most biochemical sensing/analysis, including in vitro biosensing, diagnostics, drug development, proteomics, and genomics. However, most of the conventional particle separation techniques are based on membrane filtration techniques, whose efficiency is limited by membrane characteristics, such as pore size, porosity, surface charge density, or biocompatibility, which results in a reduction in the separation efficiency of bioparticles of various sizes and types. In addition, since other conventional separation methods, such as centrifugation, chromatography, and precipitation, are difficult to perform in a continuous manner, requiring multiple preparation steps with a relatively large minimum sample volume is necessary for stable bioprocessing. Recently, microfluidic engineering enables more efficient separation in a continuous flow with rapid processing of small volumes of rare biological samples, such as DNA, proteins, viruses, exosomes, and even cells. In this paper, we present a comprehensive review of the recent advances in microfluidic separation of micro-/nano-sized bioparticles by summarizing the physical principles behind the separation system and practical examples of biomedical applications.
format	article
author	Se-woon Choe Bumjoo Kim Minseok Kim
author_facet	Se-woon Choe Bumjoo Kim Minseok Kim
author_sort	Se-woon Choe
title	Progress of Microfluidic Continuous Separation Techniques for Micro-/Nanoscale Bioparticles
title_short	Progress of Microfluidic Continuous Separation Techniques for Micro-/Nanoscale Bioparticles
title_full	Progress of Microfluidic Continuous Separation Techniques for Micro-/Nanoscale Bioparticles
title_fullStr	Progress of Microfluidic Continuous Separation Techniques for Micro-/Nanoscale Bioparticles
title_full_unstemmed	Progress of Microfluidic Continuous Separation Techniques for Micro-/Nanoscale Bioparticles
title_sort	progress of microfluidic continuous separation techniques for micro-/nanoscale bioparticles
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/683ae55591fa4056b32fe798900bd68d
work_keys_str_mv	AT sewoonchoe progressofmicrofluidiccontinuousseparationtechniquesformicronanoscalebioparticles AT bumjookim progressofmicrofluidiccontinuousseparationtechniquesformicronanoscalebioparticles AT minseokkim progressofmicrofluidiccontinuousseparationtechniquesformicronanoscalebioparticles
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Progress of Microfluidic Continuous Separation Techniques for Micro-/Nanoscale Bioparticles

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