Biosynthesis of zinc oxide nanoparticles using Albizia lebbeck stem bark, and evaluation of its antimicrobial, antioxidant, and cytotoxic activities on human breast cancer cell lines

Huzaifa Umar,1–3 Doga Kavaz,1–3 Nahit Rizaner1–3 1Department of Bioengineering, Institute of Graduate Studies and Research, Cyprus International University, Mersin, Turkey; 2Biotechnology Research Center, Cyprus International University, Mersin, Turkey; 3Bioengineering...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Umar H, Kavaz D, Rizaner N
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Biosynthesis antimicrobial antioxidant and anti-proliferative. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6845a925a0a04c059f64430a14cb1db2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6845a925a0a04c059f64430a14cb1db2
record_format	dspace
spelling	oai:doaj.org-article:6845a925a0a04c059f64430a14cb1db22021-12-02T01:13:24ZBiosynthesis of zinc oxide nanoparticles using Albizia lebbeck stem bark, and evaluation of its antimicrobial, antioxidant, and cytotoxic activities on human breast cancer cell lines1178-2013https://doaj.org/article/6845a925a0a04c059f64430a14cb1db22018-12-01T00:00:00Zhttps://www.dovepress.com/biosynthesis-of-zinc-oxide-nanoparticles-using-albizia-lebbeck-stem-ba-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Huzaifa Umar,1–3 Doga Kavaz,1–3 Nahit Rizaner1–3 1Department of Bioengineering, Institute of Graduate Studies and Research, Cyprus International University, Mersin, Turkey; 2Biotechnology Research Center, Cyprus International University, Mersin, Turkey; 3Bioengineering Department, Cyprus International University, Mersin, Turkey Background: Biocompatibility and stability of zinc oxide nanoparticles (ZnO NPs) synthesized using plants is an interesting research area of study in nanotechnology, due to its wide applications in biomedical, industrial, cell imaging, and biosensor fields. The present study reports the novel green synthesis of stable ZnO NPs using various concentrations of zinc nitrate (0.01M, 0.05M, 0.1M) and Albizia lebbeck stem bark extracts as an efficient chelating agent. Antimicrobial, antioxidant, cytotoxic, and antiproliferative activities of the synthesized NPs on human breast cancer cell lines were evaluated using different assays. Methods: Characterization of the synthesized ZnO NPs were carried out using various spectroscopic and microscopic techniques. Antimicrobial activity evaluation using disc diffusion method, antioxidant activity using hydrogen peroxide (H2O2) free radical scavenging assay and cytotoxic activity on MDA-MB 231 and MCF-7 using tryphan blue dye exclusion and MTT assay. Results: The UV–vis spectroscopy result revealed an absorption peak in the range of 370 nm. The involvements of A. lebbeck bioactive compounds in the stabilization of the ZnO NPs were confirmed by X-ray diffraction and Fourier transform infrared analysis. Zeta sizer studies showed an average size of 66.25 nm with a polydisparity index of 0.262. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) analyses results revealed irregular spherical morphology and the presence of primarily Zn, C, O, Na, P, and K, respectively. The biosynthesized ZnO NPs revealed strong antimicrobial potentials against various gram-negative and gram-positive bacterial pathogens. Antioxidant activities carried out using H2O2 free radical scavenging assay revealed higher IC50 values of 48.5, 48.7, and 60.2 µg/mL for 0.1M, 0.05M, and 0.01M ZnO NPs, respectively. Moreover, the biosynthesized ZnO NPs showed significant cytotoxic effects on MDA-MB 231 and MCF-7 breast cancer cell lines (P< 0.001, n≥3) in a concentration-dependent manner. Conclusion: Overall, various concentrations of ZnO NPs were synthesized through a stable, simple, and eco-friendly green route via the use of A. lebbeck stem bark extract. The biosynthesized ZnO NPs showed strong antimicrobial, antioxidant and cytotoxic activity against strongly and weakly metastatic breast cancer cell lines. Keywords: biosynthesis, antimicrobial, antioxidant, cytotoxic, biocompatibilityUmar HKavaz DRizaner NDove Medical PressarticleBiosynthesisantimicrobialantioxidant and anti-proliferative.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 87-100 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biosynthesis antimicrobial antioxidant and anti-proliferative. Medicine (General) R5-920
spellingShingle	Biosynthesis antimicrobial antioxidant and anti-proliferative. Medicine (General) R5-920 Umar H Kavaz D Rizaner N Biosynthesis of zinc oxide nanoparticles using Albizia lebbeck stem bark, and evaluation of its antimicrobial, antioxidant, and cytotoxic activities on human breast cancer cell lines
description	Huzaifa Umar,1–3 Doga Kavaz,1–3 Nahit Rizaner1–3 1Department of Bioengineering, Institute of Graduate Studies and Research, Cyprus International University, Mersin, Turkey; 2Biotechnology Research Center, Cyprus International University, Mersin, Turkey; 3Bioengineering Department, Cyprus International University, Mersin, Turkey Background: Biocompatibility and stability of zinc oxide nanoparticles (ZnO NPs) synthesized using plants is an interesting research area of study in nanotechnology, due to its wide applications in biomedical, industrial, cell imaging, and biosensor fields. The present study reports the novel green synthesis of stable ZnO NPs using various concentrations of zinc nitrate (0.01M, 0.05M, 0.1M) and Albizia lebbeck stem bark extracts as an efficient chelating agent. Antimicrobial, antioxidant, cytotoxic, and antiproliferative activities of the synthesized NPs on human breast cancer cell lines were evaluated using different assays. Methods: Characterization of the synthesized ZnO NPs were carried out using various spectroscopic and microscopic techniques. Antimicrobial activity evaluation using disc diffusion method, antioxidant activity using hydrogen peroxide (H2O2) free radical scavenging assay and cytotoxic activity on MDA-MB 231 and MCF-7 using tryphan blue dye exclusion and MTT assay. Results: The UV–vis spectroscopy result revealed an absorption peak in the range of 370 nm. The involvements of A. lebbeck bioactive compounds in the stabilization of the ZnO NPs were confirmed by X-ray diffraction and Fourier transform infrared analysis. Zeta sizer studies showed an average size of 66.25 nm with a polydisparity index of 0.262. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) analyses results revealed irregular spherical morphology and the presence of primarily Zn, C, O, Na, P, and K, respectively. The biosynthesized ZnO NPs revealed strong antimicrobial potentials against various gram-negative and gram-positive bacterial pathogens. Antioxidant activities carried out using H2O2 free radical scavenging assay revealed higher IC50 values of 48.5, 48.7, and 60.2 µg/mL for 0.1M, 0.05M, and 0.01M ZnO NPs, respectively. Moreover, the biosynthesized ZnO NPs showed significant cytotoxic effects on MDA-MB 231 and MCF-7 breast cancer cell lines (P< 0.001, n≥3) in a concentration-dependent manner. Conclusion: Overall, various concentrations of ZnO NPs were synthesized through a stable, simple, and eco-friendly green route via the use of A. lebbeck stem bark extract. The biosynthesized ZnO NPs showed strong antimicrobial, antioxidant and cytotoxic activity against strongly and weakly metastatic breast cancer cell lines. Keywords: biosynthesis, antimicrobial, antioxidant, cytotoxic, biocompatibility
format	article
author	Umar H Kavaz D Rizaner N
author_facet	Umar H Kavaz D Rizaner N
author_sort	Umar H
title	Biosynthesis of zinc oxide nanoparticles using Albizia lebbeck stem bark, and evaluation of its antimicrobial, antioxidant, and cytotoxic activities on human breast cancer cell lines
title_short	Biosynthesis of zinc oxide nanoparticles using Albizia lebbeck stem bark, and evaluation of its antimicrobial, antioxidant, and cytotoxic activities on human breast cancer cell lines
title_full	Biosynthesis of zinc oxide nanoparticles using Albizia lebbeck stem bark, and evaluation of its antimicrobial, antioxidant, and cytotoxic activities on human breast cancer cell lines
title_fullStr	Biosynthesis of zinc oxide nanoparticles using Albizia lebbeck stem bark, and evaluation of its antimicrobial, antioxidant, and cytotoxic activities on human breast cancer cell lines
title_full_unstemmed	Biosynthesis of zinc oxide nanoparticles using Albizia lebbeck stem bark, and evaluation of its antimicrobial, antioxidant, and cytotoxic activities on human breast cancer cell lines
title_sort	biosynthesis of zinc oxide nanoparticles using albizia lebbeck stem bark, and evaluation of its antimicrobial, antioxidant, and cytotoxic activities on human breast cancer cell lines
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/6845a925a0a04c059f64430a14cb1db2
work_keys_str_mv	AT umarh biosynthesisofzincoxidenanoparticlesusingalbizialebbeckstembarkandevaluationofitsantimicrobialantioxidantandcytotoxicactivitiesonhumanbreastcancercelllines AT kavazd biosynthesisofzincoxidenanoparticlesusingalbizialebbeckstembarkandevaluationofitsantimicrobialantioxidantandcytotoxicactivitiesonhumanbreastcancercelllines AT rizanern biosynthesisofzincoxidenanoparticlesusingalbizialebbeckstembarkandevaluationofitsantimicrobialantioxidantandcytotoxicactivitiesonhumanbreastcancercelllines
_version_	1718403205370478592

Biosynthesis of zinc oxide nanoparticles using Albizia lebbeck stem bark, and evaluation of its antimicrobial, antioxidant, and cytotoxic activities on human breast cancer cell lines

Ejemplares similares