Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons.

Correlating in vivo imaging of neurons and their synaptic connections with electron microscopy combines dynamic and ultrastructural information. Here we describe a semi-automated technique whereby volumes of brain tissue containing axons and dendrites, previously studied in vivo, are subsequently im...

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Autores principales:	Bohumil Maco, Anthony Holtmaat, Marco Cantoni, Anna Kreshuk, Christoph N Straehle, Fred A Hamprecht, Graham W Knott
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6849b4d76c4c4c17a00640ffd8144f7b
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spelling	oai:doaj.org-article:6849b4d76c4c4c17a00640ffd8144f7b2021-11-18T07:55:32ZCorrelative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons.1932-620310.1371/journal.pone.0057405https://doaj.org/article/6849b4d76c4c4c17a00640ffd8144f7b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23468982/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Correlating in vivo imaging of neurons and their synaptic connections with electron microscopy combines dynamic and ultrastructural information. Here we describe a semi-automated technique whereby volumes of brain tissue containing axons and dendrites, previously studied in vivo, are subsequently imaged in three dimensions with focused ion beam scanning electron microcopy. These neurites are then identified and reconstructed automatically from the image series using the latest segmentation algorithms. The fast and reliable imaging and reconstruction technique avoids any specific labeling to identify the features of interest in the electron microscope, and optimises their preservation and staining for 3D analysis.Bohumil MacoAnthony HoltmaatMarco CantoniAnna KreshukChristoph N StraehleFred A HamprechtGraham W KnottPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e57405 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bohumil Maco Anthony Holtmaat Marco Cantoni Anna Kreshuk Christoph N Straehle Fred A Hamprecht Graham W Knott Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons.
description	Correlating in vivo imaging of neurons and their synaptic connections with electron microscopy combines dynamic and ultrastructural information. Here we describe a semi-automated technique whereby volumes of brain tissue containing axons and dendrites, previously studied in vivo, are subsequently imaged in three dimensions with focused ion beam scanning electron microcopy. These neurites are then identified and reconstructed automatically from the image series using the latest segmentation algorithms. The fast and reliable imaging and reconstruction technique avoids any specific labeling to identify the features of interest in the electron microscope, and optimises their preservation and staining for 3D analysis.
format	article
author	Bohumil Maco Anthony Holtmaat Marco Cantoni Anna Kreshuk Christoph N Straehle Fred A Hamprecht Graham W Knott
author_facet	Bohumil Maco Anthony Holtmaat Marco Cantoni Anna Kreshuk Christoph N Straehle Fred A Hamprecht Graham W Knott
author_sort	Bohumil Maco
title	Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons.
title_short	Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons.
title_full	Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons.
title_fullStr	Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons.
title_full_unstemmed	Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons.
title_sort	correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/6849b4d76c4c4c17a00640ffd8144f7b
work_keys_str_mv	AT bohumilmaco correlativeinvivo2photonandfocusedionbeamscanningelectronmicroscopyofcorticalneurons AT anthonyholtmaat correlativeinvivo2photonandfocusedionbeamscanningelectronmicroscopyofcorticalneurons AT marcocantoni correlativeinvivo2photonandfocusedionbeamscanningelectronmicroscopyofcorticalneurons AT annakreshuk correlativeinvivo2photonandfocusedionbeamscanningelectronmicroscopyofcorticalneurons AT christophnstraehle correlativeinvivo2photonandfocusedionbeamscanningelectronmicroscopyofcorticalneurons AT fredahamprecht correlativeinvivo2photonandfocusedionbeamscanningelectronmicroscopyofcorticalneurons AT grahamwknott correlativeinvivo2photonandfocusedionbeamscanningelectronmicroscopyofcorticalneurons
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Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons.

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