Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons.
Correlating in vivo imaging of neurons and their synaptic connections with electron microscopy combines dynamic and ultrastructural information. Here we describe a semi-automated technique whereby volumes of brain tissue containing axons and dendrites, previously studied in vivo, are subsequently im...
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Main Authors: | Bohumil Maco, Anthony Holtmaat, Marco Cantoni, Anna Kreshuk, Christoph N Straehle, Fred A Hamprecht, Graham W Knott |
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Format: | article |
Language: | EN |
Published: |
Public Library of Science (PLoS)
2013
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Online Access: | https://doaj.org/article/6849b4d76c4c4c17a00640ffd8144f7b |
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