Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle

Abstract The zoonotic enterohemorrhagic Escherichia coli (EHEC) O157: H7 bacterium causes diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS) in humans. Cattle are primary reservoirs and EHEC O157: H7; the bacteria predominately inhabit the colon and recto-anal junctions (RAJ). The ea...

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Autores principales:	Mariano Larzábal, Wanderson Marques Da Silva, Anmol Multani, Lucas E. Vagnoni, Dadin P. Moore, Maia S. Marin, Nahuel A. Riviere, Fernando O. Delgado, Daniel A. Vilte, Matias Romero Victorica, Tao Ma, Luo Le Guan, Paola Talia, Angel Cataldi, Eduardo R. Cobo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/684ce949019a4947a8777d93b4f24afa
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spelling	oai:doaj.org-article:684ce949019a4947a8777d93b4f24afa2021-12-02T15:11:51ZEarly immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle10.1038/s41598-020-78752-x2045-2322https://doaj.org/article/684ce949019a4947a8777d93b4f24afa2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78752-xhttps://doaj.org/toc/2045-2322Abstract The zoonotic enterohemorrhagic Escherichia coli (EHEC) O157: H7 bacterium causes diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS) in humans. Cattle are primary reservoirs and EHEC O157: H7; the bacteria predominately inhabit the colon and recto-anal junctions (RAJ). The early innate immune reactions in the infected gut are critical in the pathogenesis of EHEC O157: H7. In this study, calves orally inoculated with EHEC O157: H7 showed infiltration of neutrophils in the lamina propria of ileum and RAJ at 7 and 14 days post-infection. Infected calves had altered mucin layer and mast cell populations across small and large intestines. There were differential transcription expressions of key bovine β defensins, tracheal antimicrobial peptide (TAP) in the ileum, and lingual antimicrobial peptide (LAP) in RAJ. The main Gram-negative bacterial/LPS signaling Toll-Like receptor 4 (TLR4) was downregulated in RAJ. Intestinal infection with EHEC O157: H7 impacted the gut bacterial communities and influenced the relative abundance of Negativibacillus and Erysipelotrichaceae in mucosa-associated bacteria in the rectum. Thus, innate immunity in the gut of calves showed unique characteristics during infection with EHEC O157: H7, which occurred in the absence of major clinical manifestations but denoted an active immunological niche.Mariano LarzábalWanderson Marques Da SilvaAnmol MultaniLucas E. VagnoniDadin P. MooreMaia S. MarinNahuel A. RiviereFernando O. DelgadoDaniel A. VilteMatias Romero VictoricaTao MaLuo Le GuanPaola TaliaAngel CataldiEduardo R. CoboNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mariano Larzábal Wanderson Marques Da Silva Anmol Multani Lucas E. Vagnoni Dadin P. Moore Maia S. Marin Nahuel A. Riviere Fernando O. Delgado Daniel A. Vilte Matias Romero Victorica Tao Ma Luo Le Guan Paola Talia Angel Cataldi Eduardo R. Cobo Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle
description	Abstract The zoonotic enterohemorrhagic Escherichia coli (EHEC) O157: H7 bacterium causes diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS) in humans. Cattle are primary reservoirs and EHEC O157: H7; the bacteria predominately inhabit the colon and recto-anal junctions (RAJ). The early innate immune reactions in the infected gut are critical in the pathogenesis of EHEC O157: H7. In this study, calves orally inoculated with EHEC O157: H7 showed infiltration of neutrophils in the lamina propria of ileum and RAJ at 7 and 14 days post-infection. Infected calves had altered mucin layer and mast cell populations across small and large intestines. There were differential transcription expressions of key bovine β defensins, tracheal antimicrobial peptide (TAP) in the ileum, and lingual antimicrobial peptide (LAP) in RAJ. The main Gram-negative bacterial/LPS signaling Toll-Like receptor 4 (TLR4) was downregulated in RAJ. Intestinal infection with EHEC O157: H7 impacted the gut bacterial communities and influenced the relative abundance of Negativibacillus and Erysipelotrichaceae in mucosa-associated bacteria in the rectum. Thus, innate immunity in the gut of calves showed unique characteristics during infection with EHEC O157: H7, which occurred in the absence of major clinical manifestations but denoted an active immunological niche.
format	article
author	Mariano Larzábal Wanderson Marques Da Silva Anmol Multani Lucas E. Vagnoni Dadin P. Moore Maia S. Marin Nahuel A. Riviere Fernando O. Delgado Daniel A. Vilte Matias Romero Victorica Tao Ma Luo Le Guan Paola Talia Angel Cataldi Eduardo R. Cobo
author_facet	Mariano Larzábal Wanderson Marques Da Silva Anmol Multani Lucas E. Vagnoni Dadin P. Moore Maia S. Marin Nahuel A. Riviere Fernando O. Delgado Daniel A. Vilte Matias Romero Victorica Tao Ma Luo Le Guan Paola Talia Angel Cataldi Eduardo R. Cobo
author_sort	Mariano Larzábal
title	Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle
title_short	Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle
title_full	Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle
title_fullStr	Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle
title_full_unstemmed	Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle
title_sort	early immune innate hallmarks and microbiome changes across the gut during escherichia coli o157: h7 infection in cattle
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/684ce949019a4947a8777d93b4f24afa
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Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle

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