Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex.

Triatomines are hematophagous insects of great epidemiological importance, since they are vectors of the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. Triatoma brasiliensis complex is a monophyletic group formed by two subspecies and six species: T. b. brasiliensis, T. b. mac...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Heloisa Pinotti, Jader de Oliveira, Amanda Ravazi, Fernanda Fernandez Madeira, Yago Visinho Dos Reis, Ana Beatriz Bortolozo de Oliveira, Maria Tercília Vilela de Azeredo-Oliveira, João Aristeu da Rosa, Kaio Cesar Chaboli Alevi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6856bbad2df84b10a3594400d8e38d7c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6856bbad2df84b10a3594400d8e38d7c
record_format	dspace
spelling	oai:doaj.org-article:6856bbad2df84b10a3594400d8e38d7c2021-12-02T20:07:52ZRevisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex.1932-620310.1371/journal.pone.0257992https://doaj.org/article/6856bbad2df84b10a3594400d8e38d7c2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257992https://doaj.org/toc/1932-6203Triatomines are hematophagous insects of great epidemiological importance, since they are vectors of the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. Triatoma brasiliensis complex is a monophyletic group formed by two subspecies and six species: T. b. brasiliensis, T. b. macromelasoma, T. bahiensis, T. juazeirensis, T. lenti, T. melanica, T. petrocchiae and T. sherlocki. The specific status of several species grouped in the T. brasiliensis complex was confirmed from experimental crossing and analysis of reproductive barriers. Thus, we perform interspecific experimental crosses between T. lenti and other species and subspecies of the T. brasiliensis complex and perform morphological analysis of the gonads and cytogenetic analysis in the homeologous chromosomes of the hybrids of first generation (F1). Besides that, we rescue all the literature data associated with the study of reproductive barriers in this monophyletic complex of species and subspecies. For all crosses performed between T. b. brasiliensis, T. b. macromelasoma, T. juazeirensis and T. melanica with T. lenti, interspecific copulas occurred (showing absence of mechanical isolation), hybrids were obtained, none of the male hybrids presented the phenomenon of gonadal dysgenesis and 100% pairing between the chromosomes homeologous of the hybrids was observed. Thus, we demonstrate that there are no pre-zygotic reproductive barriers installed between T. lenti and the species and subspecies of the T. brasiliensis complex. In addition, we demonstrate that the hybrids obtained between these crosses have high genomic compatibility and the absence of gonadal dysgenesis. These results point to reproductive compatibility between T. lenti and species and subspecies of the T. brasiliensis complex (confirming its inclusion in the complex) and lead us to suggest a possible recent diversification of the taxa of this monophyletic group.Heloisa PinottiJader de OliveiraAmanda RavaziFernanda Fernandez MadeiraYago Visinho Dos ReisAna Beatriz Bortolozo de OliveiraMaria Tercília Vilela de Azeredo-OliveiraJoão Aristeu da RosaKaio Cesar Chaboli AleviPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0257992 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Heloisa Pinotti Jader de Oliveira Amanda Ravazi Fernanda Fernandez Madeira Yago Visinho Dos Reis Ana Beatriz Bortolozo de Oliveira Maria Tercília Vilela de Azeredo-Oliveira João Aristeu da Rosa Kaio Cesar Chaboli Alevi Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex.
description	Triatomines are hematophagous insects of great epidemiological importance, since they are vectors of the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. Triatoma brasiliensis complex is a monophyletic group formed by two subspecies and six species: T. b. brasiliensis, T. b. macromelasoma, T. bahiensis, T. juazeirensis, T. lenti, T. melanica, T. petrocchiae and T. sherlocki. The specific status of several species grouped in the T. brasiliensis complex was confirmed from experimental crossing and analysis of reproductive barriers. Thus, we perform interspecific experimental crosses between T. lenti and other species and subspecies of the T. brasiliensis complex and perform morphological analysis of the gonads and cytogenetic analysis in the homeologous chromosomes of the hybrids of first generation (F1). Besides that, we rescue all the literature data associated with the study of reproductive barriers in this monophyletic complex of species and subspecies. For all crosses performed between T. b. brasiliensis, T. b. macromelasoma, T. juazeirensis and T. melanica with T. lenti, interspecific copulas occurred (showing absence of mechanical isolation), hybrids were obtained, none of the male hybrids presented the phenomenon of gonadal dysgenesis and 100% pairing between the chromosomes homeologous of the hybrids was observed. Thus, we demonstrate that there are no pre-zygotic reproductive barriers installed between T. lenti and the species and subspecies of the T. brasiliensis complex. In addition, we demonstrate that the hybrids obtained between these crosses have high genomic compatibility and the absence of gonadal dysgenesis. These results point to reproductive compatibility between T. lenti and species and subspecies of the T. brasiliensis complex (confirming its inclusion in the complex) and lead us to suggest a possible recent diversification of the taxa of this monophyletic group.
format	article
author	Heloisa Pinotti Jader de Oliveira Amanda Ravazi Fernanda Fernandez Madeira Yago Visinho Dos Reis Ana Beatriz Bortolozo de Oliveira Maria Tercília Vilela de Azeredo-Oliveira João Aristeu da Rosa Kaio Cesar Chaboli Alevi
author_facet	Heloisa Pinotti Jader de Oliveira Amanda Ravazi Fernanda Fernandez Madeira Yago Visinho Dos Reis Ana Beatriz Bortolozo de Oliveira Maria Tercília Vilela de Azeredo-Oliveira João Aristeu da Rosa Kaio Cesar Chaboli Alevi
author_sort	Heloisa Pinotti
title	Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex.
title_short	Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex.
title_full	Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex.
title_fullStr	Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex.
title_full_unstemmed	Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex.
title_sort	revisiting the hybridization processes in the triatoma brasiliensis complex (hemiptera, triatominae): interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/6856bbad2df84b10a3594400d8e38d7c
work_keys_str_mv	AT heloisapinotti revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaeinterspecificgenomiccompatibilitypointtoapossiblerecentdiversificationofthespeciesgroupedinthismonophyleticcomplex AT jaderdeoliveira revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaeinterspecificgenomiccompatibilitypointtoapossiblerecentdiversificationofthespeciesgroupedinthismonophyleticcomplex AT amandaravazi revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaeinterspecificgenomiccompatibilitypointtoapossiblerecentdiversificationofthespeciesgroupedinthismonophyleticcomplex AT fernandafernandezmadeira revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaeinterspecificgenomiccompatibilitypointtoapossiblerecentdiversificationofthespeciesgroupedinthismonophyleticcomplex AT yagovisinhodosreis revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaeinterspecificgenomiccompatibilitypointtoapossiblerecentdiversificationofthespeciesgroupedinthismonophyleticcomplex AT anabeatrizbortolozodeoliveira revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaeinterspecificgenomiccompatibilitypointtoapossiblerecentdiversificationofthespeciesgroupedinthismonophyleticcomplex AT mariaterciliavileladeazeredooliveira revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaeinterspecificgenomiccompatibilitypointtoapossiblerecentdiversificationofthespeciesgroupedinthismonophyleticcomplex AT joaoaristeudarosa revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaeinterspecificgenomiccompatibilitypointtoapossiblerecentdiversificationofthespeciesgroupedinthismonophyleticcomplex AT kaiocesarchabolialevi revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaeinterspecificgenomiccompatibilitypointtoapossiblerecentdiversificationofthespeciesgroupedinthismonophyleticcomplex
_version_	1718375245528694784

Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex.

Ejemplares similares