Size-dependent toxicity of silver nanoparticles to bacteria, yeast, algae, crustaceans and mammalian cells in vitro.

The concept of nanotechnologies is based on size-dependent properties of particles in the 1-100 nm range. However, the relation between the particle size and biological effects is still unclear. The aim of the current paper was to generate and analyse a homogenous set of experimental toxicity data o...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Angela Ivask, Imbi Kurvet, Kaja Kasemets, Irina Blinova, Villem Aruoja, Sandra Suppi, Heiki Vija, Aleksandr Käkinen, Tiina Titma, Margit Heinlaan, Meeri Visnapuu, Dagmar Koller, Vambola Kisand, Anne Kahru
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2014
Materias:
R
Q
Acceso en línea:https://doaj.org/article/685bca567b5541d98965fa4d85bf4d71
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:685bca567b5541d98965fa4d85bf4d71
record_format dspace
spelling oai:doaj.org-article:685bca567b5541d98965fa4d85bf4d712021-11-25T06:07:45ZSize-dependent toxicity of silver nanoparticles to bacteria, yeast, algae, crustaceans and mammalian cells in vitro.1932-620310.1371/journal.pone.0102108https://doaj.org/article/685bca567b5541d98965fa4d85bf4d712014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25048192/?tool=EBIhttps://doaj.org/toc/1932-6203The concept of nanotechnologies is based on size-dependent properties of particles in the 1-100 nm range. However, the relation between the particle size and biological effects is still unclear. The aim of the current paper was to generate and analyse a homogenous set of experimental toxicity data on Ag nanoparticles (Ag NPs) of similar coating (citrate) but of 5 different primary sizes (10, 20, 40, 60 and 80 nm) to different types of organisms/cells commonly used in toxicity assays: bacterial, yeast and algal cells, crustaceans and mammalian cells in vitro. When possible, the assays were conducted in ultrapure water to minimise the effect of medium components on silver speciation. The toxic effects of NPs to different organisms varied about two orders of magnitude, being the lowest (∼0.1 mg Ag/L) for crustaceans and algae and the highest (∼26 mg Ag/L) for mammalian cells. To quantify the role of Ag ions in the toxicity of Ag NPs, we normalized the EC50 values to Ag ions that dissolved from the NPs. The analysis showed that the toxicity of 20-80 nm Ag NPs could fully be explained by released Ag ions whereas 10 nm Ag NPs proved more toxic than predicted. Using E. coli Ag-biosensor, we demonstrated that 10 nm Ag NPs were more bioavailable to E. coli than silver salt (AgNO3). Thus, one may infer that 10 nm Ag NPs had more efficient cell-particle contact resulting in higher intracellular bioavailability of silver than in case of bigger NPs. Although the latter conclusion is initially based on one test organism, it may lead to an explanation for "size-dependent" biological effects of silver NPs. This study, for the first time, investigated the size-dependent toxic effects of a well-characterized library of Ag NPs to several microbial species, protozoans, algae, crustaceans and mammalian cells in vitro.Angela IvaskImbi KurvetKaja KasemetsIrina BlinovaVillem AruojaSandra SuppiHeiki VijaAleksandr KäkinenTiina TitmaMargit HeinlaanMeeri VisnapuuDagmar KollerVambola KisandAnne KahruPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e102108 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Angela Ivask
Imbi Kurvet
Kaja Kasemets
Irina Blinova
Villem Aruoja
Sandra Suppi
Heiki Vija
Aleksandr Käkinen
Tiina Titma
Margit Heinlaan
Meeri Visnapuu
Dagmar Koller
Vambola Kisand
Anne Kahru
Size-dependent toxicity of silver nanoparticles to bacteria, yeast, algae, crustaceans and mammalian cells in vitro.
description The concept of nanotechnologies is based on size-dependent properties of particles in the 1-100 nm range. However, the relation between the particle size and biological effects is still unclear. The aim of the current paper was to generate and analyse a homogenous set of experimental toxicity data on Ag nanoparticles (Ag NPs) of similar coating (citrate) but of 5 different primary sizes (10, 20, 40, 60 and 80 nm) to different types of organisms/cells commonly used in toxicity assays: bacterial, yeast and algal cells, crustaceans and mammalian cells in vitro. When possible, the assays were conducted in ultrapure water to minimise the effect of medium components on silver speciation. The toxic effects of NPs to different organisms varied about two orders of magnitude, being the lowest (∼0.1 mg Ag/L) for crustaceans and algae and the highest (∼26 mg Ag/L) for mammalian cells. To quantify the role of Ag ions in the toxicity of Ag NPs, we normalized the EC50 values to Ag ions that dissolved from the NPs. The analysis showed that the toxicity of 20-80 nm Ag NPs could fully be explained by released Ag ions whereas 10 nm Ag NPs proved more toxic than predicted. Using E. coli Ag-biosensor, we demonstrated that 10 nm Ag NPs were more bioavailable to E. coli than silver salt (AgNO3). Thus, one may infer that 10 nm Ag NPs had more efficient cell-particle contact resulting in higher intracellular bioavailability of silver than in case of bigger NPs. Although the latter conclusion is initially based on one test organism, it may lead to an explanation for "size-dependent" biological effects of silver NPs. This study, for the first time, investigated the size-dependent toxic effects of a well-characterized library of Ag NPs to several microbial species, protozoans, algae, crustaceans and mammalian cells in vitro.
format article
author Angela Ivask
Imbi Kurvet
Kaja Kasemets
Irina Blinova
Villem Aruoja
Sandra Suppi
Heiki Vija
Aleksandr Käkinen
Tiina Titma
Margit Heinlaan
Meeri Visnapuu
Dagmar Koller
Vambola Kisand
Anne Kahru
author_facet Angela Ivask
Imbi Kurvet
Kaja Kasemets
Irina Blinova
Villem Aruoja
Sandra Suppi
Heiki Vija
Aleksandr Käkinen
Tiina Titma
Margit Heinlaan
Meeri Visnapuu
Dagmar Koller
Vambola Kisand
Anne Kahru
author_sort Angela Ivask
title Size-dependent toxicity of silver nanoparticles to bacteria, yeast, algae, crustaceans and mammalian cells in vitro.
title_short Size-dependent toxicity of silver nanoparticles to bacteria, yeast, algae, crustaceans and mammalian cells in vitro.
title_full Size-dependent toxicity of silver nanoparticles to bacteria, yeast, algae, crustaceans and mammalian cells in vitro.
title_fullStr Size-dependent toxicity of silver nanoparticles to bacteria, yeast, algae, crustaceans and mammalian cells in vitro.
title_full_unstemmed Size-dependent toxicity of silver nanoparticles to bacteria, yeast, algae, crustaceans and mammalian cells in vitro.
title_sort size-dependent toxicity of silver nanoparticles to bacteria, yeast, algae, crustaceans and mammalian cells in vitro.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/685bca567b5541d98965fa4d85bf4d71
work_keys_str_mv AT angelaivask sizedependenttoxicityofsilvernanoparticlestobacteriayeastalgaecrustaceansandmammaliancellsinvitro
AT imbikurvet sizedependenttoxicityofsilvernanoparticlestobacteriayeastalgaecrustaceansandmammaliancellsinvitro
AT kajakasemets sizedependenttoxicityofsilvernanoparticlestobacteriayeastalgaecrustaceansandmammaliancellsinvitro
AT irinablinova sizedependenttoxicityofsilvernanoparticlestobacteriayeastalgaecrustaceansandmammaliancellsinvitro
AT villemaruoja sizedependenttoxicityofsilvernanoparticlestobacteriayeastalgaecrustaceansandmammaliancellsinvitro
AT sandrasuppi sizedependenttoxicityofsilvernanoparticlestobacteriayeastalgaecrustaceansandmammaliancellsinvitro
AT heikivija sizedependenttoxicityofsilvernanoparticlestobacteriayeastalgaecrustaceansandmammaliancellsinvitro
AT aleksandrkakinen sizedependenttoxicityofsilvernanoparticlestobacteriayeastalgaecrustaceansandmammaliancellsinvitro
AT tiinatitma sizedependenttoxicityofsilvernanoparticlestobacteriayeastalgaecrustaceansandmammaliancellsinvitro
AT margitheinlaan sizedependenttoxicityofsilvernanoparticlestobacteriayeastalgaecrustaceansandmammaliancellsinvitro
AT meerivisnapuu sizedependenttoxicityofsilvernanoparticlestobacteriayeastalgaecrustaceansandmammaliancellsinvitro
AT dagmarkoller sizedependenttoxicityofsilvernanoparticlestobacteriayeastalgaecrustaceansandmammaliancellsinvitro
AT vambolakisand sizedependenttoxicityofsilvernanoparticlestobacteriayeastalgaecrustaceansandmammaliancellsinvitro
AT annekahru sizedependenttoxicityofsilvernanoparticlestobacteriayeastalgaecrustaceansandmammaliancellsinvitro
_version_ 1718414167948394496