An octopamine receptor confers selective toxicity of amitraz on honeybees and Varroa mites

The Varroa destructor mite is a devastating parasite of Apis mellifera honeybees. They can cause colonies to collapse by spreading viruses and feeding on the fat reserves of adults and larvae. Amitraz is used to control mites due to its low toxicity to bees; however, the mechanism of bee resistance...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lei Guo, Xin-yu Fan, Xiaomu Qiao, Craig Montell, Jia Huang
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	honeybee Varroa mite octopamine receptor amitraz selective toxicity resistance Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/68679e9f1031475b8776695e9ccde68e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:68679e9f1031475b8776695e9ccde68e
record_format	dspace
spelling	oai:doaj.org-article:68679e9f1031475b8776695e9ccde68e2021-11-25T10:22:39ZAn octopamine receptor confers selective toxicity of amitraz on honeybees and Varroa mites10.7554/eLife.682682050-084Xe68268https://doaj.org/article/68679e9f1031475b8776695e9ccde68e2021-07-01T00:00:00Zhttps://elifesciences.org/articles/68268https://doaj.org/toc/2050-084XThe Varroa destructor mite is a devastating parasite of Apis mellifera honeybees. They can cause colonies to collapse by spreading viruses and feeding on the fat reserves of adults and larvae. Amitraz is used to control mites due to its low toxicity to bees; however, the mechanism of bee resistance to amitraz remains unknown. In this study, we found that amitraz and its major metabolite potently activated all four mite octopamine receptors. Behavioral assays using Drosophila null mutants of octopamine receptors identified one receptor subtype Octβ2R as the sole target of amitraz in vivo. We found that thermogenetic activation of octβ2R-expressing neurons mimics amitraz poisoning symptoms in target pests. We next confirmed that the mite Octβ2R was more sensitive to amitraz and its metabolite than the bee Octβ2R in pharmacological assays and transgenic flies. Furthermore, replacement of three bee-specific residues with the counterparts in the mite receptor increased amitraz sensitivity of the bee Octβ2R, indicating that the relative insensitivity of their receptor is the major mechanism for honeybees to resist amitraz. The present findings have important implications for resistance management and the design of safer insecticides that selectively target pests while maintaining low toxicity to non-target pollinators.Lei GuoXin-yu FanXiaomu QiaoCraig MontellJia HuangeLife Sciences Publications LtdarticlehoneybeeVarroa miteoctopamine receptoramitrazselective toxicityresistanceMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	honeybee Varroa mite octopamine receptor amitraz selective toxicity resistance Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	honeybee Varroa mite octopamine receptor amitraz selective toxicity resistance Medicine R Science Q Biology (General) QH301-705.5 Lei Guo Xin-yu Fan Xiaomu Qiao Craig Montell Jia Huang An octopamine receptor confers selective toxicity of amitraz on honeybees and Varroa mites
description	The Varroa destructor mite is a devastating parasite of Apis mellifera honeybees. They can cause colonies to collapse by spreading viruses and feeding on the fat reserves of adults and larvae. Amitraz is used to control mites due to its low toxicity to bees; however, the mechanism of bee resistance to amitraz remains unknown. In this study, we found that amitraz and its major metabolite potently activated all four mite octopamine receptors. Behavioral assays using Drosophila null mutants of octopamine receptors identified one receptor subtype Octβ2R as the sole target of amitraz in vivo. We found that thermogenetic activation of octβ2R-expressing neurons mimics amitraz poisoning symptoms in target pests. We next confirmed that the mite Octβ2R was more sensitive to amitraz and its metabolite than the bee Octβ2R in pharmacological assays and transgenic flies. Furthermore, replacement of three bee-specific residues with the counterparts in the mite receptor increased amitraz sensitivity of the bee Octβ2R, indicating that the relative insensitivity of their receptor is the major mechanism for honeybees to resist amitraz. The present findings have important implications for resistance management and the design of safer insecticides that selectively target pests while maintaining low toxicity to non-target pollinators.
format	article
author	Lei Guo Xin-yu Fan Xiaomu Qiao Craig Montell Jia Huang
author_facet	Lei Guo Xin-yu Fan Xiaomu Qiao Craig Montell Jia Huang
author_sort	Lei Guo
title	An octopamine receptor confers selective toxicity of amitraz on honeybees and Varroa mites
title_short	An octopamine receptor confers selective toxicity of amitraz on honeybees and Varroa mites
title_full	An octopamine receptor confers selective toxicity of amitraz on honeybees and Varroa mites
title_fullStr	An octopamine receptor confers selective toxicity of amitraz on honeybees and Varroa mites
title_full_unstemmed	An octopamine receptor confers selective toxicity of amitraz on honeybees and Varroa mites
title_sort	octopamine receptor confers selective toxicity of amitraz on honeybees and varroa mites
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/68679e9f1031475b8776695e9ccde68e
work_keys_str_mv	AT leiguo anoctopaminereceptorconfersselectivetoxicityofamitrazonhoneybeesandvarroamites AT xinyufan anoctopaminereceptorconfersselectivetoxicityofamitrazonhoneybeesandvarroamites AT xiaomuqiao anoctopaminereceptorconfersselectivetoxicityofamitrazonhoneybeesandvarroamites AT craigmontell anoctopaminereceptorconfersselectivetoxicityofamitrazonhoneybeesandvarroamites AT jiahuang anoctopaminereceptorconfersselectivetoxicityofamitrazonhoneybeesandvarroamites AT leiguo octopaminereceptorconfersselectivetoxicityofamitrazonhoneybeesandvarroamites AT xinyufan octopaminereceptorconfersselectivetoxicityofamitrazonhoneybeesandvarroamites AT xiaomuqiao octopaminereceptorconfersselectivetoxicityofamitrazonhoneybeesandvarroamites AT craigmontell octopaminereceptorconfersselectivetoxicityofamitrazonhoneybeesandvarroamites AT jiahuang octopaminereceptorconfersselectivetoxicityofamitrazonhoneybeesandvarroamites
_version_	1718413561415335936

An octopamine receptor confers selective toxicity of amitraz on honeybees and Varroa mites

Ejemplares similares