Wide-field calcium imaging of cortex-wide activity in awake, head-fixed mice

Summary: Characterizing cortex-wide neural activity is essential for understanding large-scale interactions among distributed cortical regions. Here, we describe a protocol using wide-field calcium imaging to monitor the cortex-wide activity in awake, head-fixed mice. This approach provides sufficie...

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Main Authors:	Chi Ren, Takaki Komiyama
Format:	article
Language:	EN
Published:	Elsevier 2021
Subjects:	Science (General) Q1-390
Online Access:	https://doaj.org/article/6875b4a8c39340afbc3ccd41db500a5d
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spelling	oai:doaj.org-article:6875b4a8c39340afbc3ccd41db500a5d2021-11-22T04:31:02ZWide-field calcium imaging of cortex-wide activity in awake, head-fixed mice2666-166710.1016/j.xpro.2021.100973https://doaj.org/article/6875b4a8c39340afbc3ccd41db500a5d2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006791https://doaj.org/toc/2666-1667Summary: Characterizing cortex-wide neural activity is essential for understanding large-scale interactions among distributed cortical regions. Here, we describe a protocol using wide-field calcium imaging to monitor the cortex-wide activity in awake, head-fixed mice. This approach provides sufficient signal-to-noise ratio and spatiotemporal resolution to capture large-scale neural activity in behaving mice on a moment-by-moment basis. The use of genetically encoded calcium indicators allows longitudinal imaging over months and can achieve cell-type specificity. We also describe a pipeline to process the imaging data.For complete details on the use and execution of this protocol, please refer to Makino et al. (2017) and Liu et al. (2021).Chi RenTakaki KomiyamaElsevierarticleScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100973- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science (General) Q1-390
spellingShingle	Science (General) Q1-390 Chi Ren Takaki Komiyama Wide-field calcium imaging of cortex-wide activity in awake, head-fixed mice
description	Summary: Characterizing cortex-wide neural activity is essential for understanding large-scale interactions among distributed cortical regions. Here, we describe a protocol using wide-field calcium imaging to monitor the cortex-wide activity in awake, head-fixed mice. This approach provides sufficient signal-to-noise ratio and spatiotemporal resolution to capture large-scale neural activity in behaving mice on a moment-by-moment basis. The use of genetically encoded calcium indicators allows longitudinal imaging over months and can achieve cell-type specificity. We also describe a pipeline to process the imaging data.For complete details on the use and execution of this protocol, please refer to Makino et al. (2017) and Liu et al. (2021).
format	article
author	Chi Ren Takaki Komiyama
author_facet	Chi Ren Takaki Komiyama
author_sort	Chi Ren
title	Wide-field calcium imaging of cortex-wide activity in awake, head-fixed mice
title_short	Wide-field calcium imaging of cortex-wide activity in awake, head-fixed mice
title_full	Wide-field calcium imaging of cortex-wide activity in awake, head-fixed mice
title_fullStr	Wide-field calcium imaging of cortex-wide activity in awake, head-fixed mice
title_full_unstemmed	Wide-field calcium imaging of cortex-wide activity in awake, head-fixed mice
title_sort	wide-field calcium imaging of cortex-wide activity in awake, head-fixed mice
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/6875b4a8c39340afbc3ccd41db500a5d
work_keys_str_mv	AT chiren widefieldcalciumimagingofcortexwideactivityinawakeheadfixedmice AT takakikomiyama widefieldcalciumimagingofcortexwideactivityinawakeheadfixedmice
_version_	1718418164814970880

Wide-field calcium imaging of cortex-wide activity in awake, head-fixed mice

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