Genetic stability of Rift Valley fever virus MP-12 vaccine during serial passages in culture cells

Rift Valley fever: Whole-virus vaccine alters the attenuation profile A vaccine candidate for Rift Valley fever virus can undergo mutations and partially revert to parental pathogenic strain. Rift Valley fever is a prolific infection that affects livestock and humans in Africa. Tetsuro Ikegami and N...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nandadeva Lokugamage, Tetsuro Ikegami
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/6877a549b3d44f31b2c263a6a429ad79
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6877a549b3d44f31b2c263a6a429ad79
record_format	dspace
spelling	oai:doaj.org-article:6877a549b3d44f31b2c263a6a429ad792021-12-02T16:06:13ZGenetic stability of Rift Valley fever virus MP-12 vaccine during serial passages in culture cells10.1038/s41541-017-0021-92059-0105https://doaj.org/article/6877a549b3d44f31b2c263a6a429ad792017-07-01T00:00:00Zhttps://doi.org/10.1038/s41541-017-0021-9https://doaj.org/toc/2059-0105Rift Valley fever: Whole-virus vaccine alters the attenuation profile A vaccine candidate for Rift Valley fever virus can undergo mutations and partially revert to parental pathogenic strain. Rift Valley fever is a prolific infection that affects livestock and humans in Africa. Tetsuro Ikegami and Nandadeva Lokugamage, of the University of Texas Medical Branch, United States, tested the genetic stability of vaccine candidate MP-12, an attenuated form of the virus, through ‘serial passaging’—culturing the virus, establishing a new culture from a sample, then repeating the procedure to determine how the virus mutates in response to successive new environments. The duo found that after 25 ‘passages’, MP-12 reverted a part of attenuation mutations back to its original, pathogenic sequence. Though MP-12 redundantly encodes stable attenuation mutations, it will be important to use a seed lot system to avoid the attenuation profile changes.Nandadeva LokugamageTetsuro IkegamiNature PortfolioarticleImmunologic diseases. AllergyRC581-607Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Vaccines, Vol 2, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Nandadeva Lokugamage Tetsuro Ikegami Genetic stability of Rift Valley fever virus MP-12 vaccine during serial passages in culture cells
description	Rift Valley fever: Whole-virus vaccine alters the attenuation profile A vaccine candidate for Rift Valley fever virus can undergo mutations and partially revert to parental pathogenic strain. Rift Valley fever is a prolific infection that affects livestock and humans in Africa. Tetsuro Ikegami and Nandadeva Lokugamage, of the University of Texas Medical Branch, United States, tested the genetic stability of vaccine candidate MP-12, an attenuated form of the virus, through ‘serial passaging’—culturing the virus, establishing a new culture from a sample, then repeating the procedure to determine how the virus mutates in response to successive new environments. The duo found that after 25 ‘passages’, MP-12 reverted a part of attenuation mutations back to its original, pathogenic sequence. Though MP-12 redundantly encodes stable attenuation mutations, it will be important to use a seed lot system to avoid the attenuation profile changes.
format	article
author	Nandadeva Lokugamage Tetsuro Ikegami
author_facet	Nandadeva Lokugamage Tetsuro Ikegami
author_sort	Nandadeva Lokugamage
title	Genetic stability of Rift Valley fever virus MP-12 vaccine during serial passages in culture cells
title_short	Genetic stability of Rift Valley fever virus MP-12 vaccine during serial passages in culture cells
title_full	Genetic stability of Rift Valley fever virus MP-12 vaccine during serial passages in culture cells
title_fullStr	Genetic stability of Rift Valley fever virus MP-12 vaccine during serial passages in culture cells
title_full_unstemmed	Genetic stability of Rift Valley fever virus MP-12 vaccine during serial passages in culture cells
title_sort	genetic stability of rift valley fever virus mp-12 vaccine during serial passages in culture cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6877a549b3d44f31b2c263a6a429ad79
work_keys_str_mv	AT nandadevalokugamage geneticstabilityofriftvalleyfevervirusmp12vaccineduringserialpassagesinculturecells AT tetsuroikegami geneticstabilityofriftvalleyfevervirusmp12vaccineduringserialpassagesinculturecells
_version_	1718385093621317632

Genetic stability of Rift Valley fever virus MP-12 vaccine during serial passages in culture cells

Ejemplares similares