Plasma apolipopotein C-2 elevation is associated with Takayasu arteritis

Abstract Takayasu arteritis (TAK) is an autoimmune systemic arteritis of unknown etiology. Although a number of investigators have attempted to determine biomarkers for diagnosing TAK, there exist no specific serological markers of this intractable disease. We undertook the exploration of novel sero...

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Autores principales:	Natsuko Tamura, Yasuhiro Maejima, Yuka Shiheido-Watanabe, Shun Nakagama, Mitsuaki Isobe, Tetsuo Sasano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/687b632b0a2d4498ac247db45c46efd2
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spelling	oai:doaj.org-article:687b632b0a2d4498ac247db45c46efd22021-12-02T18:14:08ZPlasma apolipopotein C-2 elevation is associated with Takayasu arteritis10.1038/s41598-021-98615-32045-2322https://doaj.org/article/687b632b0a2d4498ac247db45c46efd22021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98615-3https://doaj.org/toc/2045-2322Abstract Takayasu arteritis (TAK) is an autoimmune systemic arteritis of unknown etiology. Although a number of investigators have attempted to determine biomarkers for diagnosing TAK, there exist no specific serological markers of this intractable disease. We undertook the exploration of novel serological markers which could be useful for an accurate diagnosis of TAK using an unbiased proteomics approach. The purified plasma samples from untreated patients with TAK and healthy individuals were separated by two-dimensional electrophoresis. The differentially expressed protein spots were detected by gel comparison and identified using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MS). Next, we validated plasma concentrations of identified proteins by enzyme-linked immunosorbent assay (ELISA). Two-dimensional electrophoresis and numerical analysis revealed 19 spots and 3 spot clusters whose sum of the sample averages was ≥ 0.01, and the average concentrations were ≥ 1.5 times in the patient group compared with the control group. Among them, 10 spots and spot clusters that met the condition of the average spot concentration being 2.5 times more than that in the control group were selected. After processing these spots using MS and conducting MS/MS ion search, we identified 10 proteins: apolipoprotein C-2 (ApoC-2), actin, apolipoprotein A-1, complement C3, kininogen-1, vitronectin, α2-macroglobulin, 14–3–3 protein ζ/δ, complement C4, and inter-α-trypsin inhibitor heavy chain H4 isoform 1 precursor. Finally, ELISA demonstrated that plasma ApoC-2 level was significantly elevated in patients with TAK compared with that in healthy individuals. Thus, ApoC-2 would be a promising candidate biomarker for TAK diagnosis.Natsuko TamuraYasuhiro MaejimaYuka Shiheido-WatanabeShun NakagamaMitsuaki IsobeTetsuo SasanoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Natsuko Tamura Yasuhiro Maejima Yuka Shiheido-Watanabe Shun Nakagama Mitsuaki Isobe Tetsuo Sasano Plasma apolipopotein C-2 elevation is associated with Takayasu arteritis
description	Abstract Takayasu arteritis (TAK) is an autoimmune systemic arteritis of unknown etiology. Although a number of investigators have attempted to determine biomarkers for diagnosing TAK, there exist no specific serological markers of this intractable disease. We undertook the exploration of novel serological markers which could be useful for an accurate diagnosis of TAK using an unbiased proteomics approach. The purified plasma samples from untreated patients with TAK and healthy individuals were separated by two-dimensional electrophoresis. The differentially expressed protein spots were detected by gel comparison and identified using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MS). Next, we validated plasma concentrations of identified proteins by enzyme-linked immunosorbent assay (ELISA). Two-dimensional electrophoresis and numerical analysis revealed 19 spots and 3 spot clusters whose sum of the sample averages was ≥ 0.01, and the average concentrations were ≥ 1.5 times in the patient group compared with the control group. Among them, 10 spots and spot clusters that met the condition of the average spot concentration being 2.5 times more than that in the control group were selected. After processing these spots using MS and conducting MS/MS ion search, we identified 10 proteins: apolipoprotein C-2 (ApoC-2), actin, apolipoprotein A-1, complement C3, kininogen-1, vitronectin, α2-macroglobulin, 14–3–3 protein ζ/δ, complement C4, and inter-α-trypsin inhibitor heavy chain H4 isoform 1 precursor. Finally, ELISA demonstrated that plasma ApoC-2 level was significantly elevated in patients with TAK compared with that in healthy individuals. Thus, ApoC-2 would be a promising candidate biomarker for TAK diagnosis.
format	article
author	Natsuko Tamura Yasuhiro Maejima Yuka Shiheido-Watanabe Shun Nakagama Mitsuaki Isobe Tetsuo Sasano
author_facet	Natsuko Tamura Yasuhiro Maejima Yuka Shiheido-Watanabe Shun Nakagama Mitsuaki Isobe Tetsuo Sasano
author_sort	Natsuko Tamura
title	Plasma apolipopotein C-2 elevation is associated with Takayasu arteritis
title_short	Plasma apolipopotein C-2 elevation is associated with Takayasu arteritis
title_full	Plasma apolipopotein C-2 elevation is associated with Takayasu arteritis
title_fullStr	Plasma apolipopotein C-2 elevation is associated with Takayasu arteritis
title_full_unstemmed	Plasma apolipopotein C-2 elevation is associated with Takayasu arteritis
title_sort	plasma apolipopotein c-2 elevation is associated with takayasu arteritis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/687b632b0a2d4498ac247db45c46efd2
work_keys_str_mv	AT natsukotamura plasmaapolipopoteinc2elevationisassociatedwithtakayasuarteritis AT yasuhiromaejima plasmaapolipopoteinc2elevationisassociatedwithtakayasuarteritis AT yukashiheidowatanabe plasmaapolipopoteinc2elevationisassociatedwithtakayasuarteritis AT shunnakagama plasmaapolipopoteinc2elevationisassociatedwithtakayasuarteritis AT mitsuakiisobe plasmaapolipopoteinc2elevationisassociatedwithtakayasuarteritis AT tetsuosasano plasmaapolipopoteinc2elevationisassociatedwithtakayasuarteritis
_version_	1718378456180326400

Plasma apolipopotein C-2 elevation is associated with Takayasu arteritis

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