Membrane Ballooning in Aggregated Platelets is Synchronised and Mediates a Surge in Microvesiculation

Abstract Human platelet transformation into balloons is part of the haemostatic response and thrombus architecture. Here we reveal that in aggregates of platelets in plasma, ballooning in multiple platelets occurs in a synchronised manner. This suggests a mechanism of coordination between cells, pre...

Description complète

Enregistré dans:

Détails bibliographiques
Auteurs principaux:	Ejaife O. Agbani, Christopher M. Williams, Ingeborg Hers, Alastair W. Poole
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2017
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/687bb9ef88c64ed9a873232060e73ace
Tags:	Ajouter un tag Pas de tags, Soyez le premier à ajouter un tag!

id	oai:doaj.org-article:687bb9ef88c64ed9a873232060e73ace
record_format	dspace
spelling	oai:doaj.org-article:687bb9ef88c64ed9a873232060e73ace2021-12-02T16:06:33ZMembrane Ballooning in Aggregated Platelets is Synchronised and Mediates a Surge in Microvesiculation10.1038/s41598-017-02933-42045-2322https://doaj.org/article/687bb9ef88c64ed9a873232060e73ace2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02933-4https://doaj.org/toc/2045-2322Abstract Human platelet transformation into balloons is part of the haemostatic response and thrombus architecture. Here we reveal that in aggregates of platelets in plasma, ballooning in multiple platelets occurs in a synchronised manner. This suggests a mechanism of coordination between cells, previously unrecognised. We aimed to understand this mechanism, and how it may contribute to thrombus development. Using spinning-disc confocal microscopy we visualised membrane ballooning in human platelet aggregates adherent to collagen-coated surfaces. Within an aggregate, multiple platelets undergo ballooning in a synchronised fashion, dependent upon extracellular calcium, in a manner that followed peak cytosolic calcium levels in the aggregate. Synchrony was observed in platelets within but not between aggregates, suggesting a level of intra-thrombus communication. Blocking phosphatidylserine, inhibiting thrombin or blocking PAR1 receptor, largely prevented synchrony without blocking ballooning itself. In contrast, inhibition of connexins, P2Y12, P2Y1 or thromboxane formation had no effect on synchrony or ballooning. Importantly, synchronised ballooning was closely followed by a surge in microvesicle formation, which was absent when synchrony was blocked. Our data demonstrate that the mechanism underlying synchronised membrane ballooning requires thrombin generation acting effectively in a positive feedback loop, mediating a subsequent surge in procoagulant activity and microvesicle release.Ejaife O. AgbaniChristopher M. WilliamsIngeborg HersAlastair W. PooleNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ejaife O. Agbani Christopher M. Williams Ingeborg Hers Alastair W. Poole Membrane Ballooning in Aggregated Platelets is Synchronised and Mediates a Surge in Microvesiculation
description	Abstract Human platelet transformation into balloons is part of the haemostatic response and thrombus architecture. Here we reveal that in aggregates of platelets in plasma, ballooning in multiple platelets occurs in a synchronised manner. This suggests a mechanism of coordination between cells, previously unrecognised. We aimed to understand this mechanism, and how it may contribute to thrombus development. Using spinning-disc confocal microscopy we visualised membrane ballooning in human platelet aggregates adherent to collagen-coated surfaces. Within an aggregate, multiple platelets undergo ballooning in a synchronised fashion, dependent upon extracellular calcium, in a manner that followed peak cytosolic calcium levels in the aggregate. Synchrony was observed in platelets within but not between aggregates, suggesting a level of intra-thrombus communication. Blocking phosphatidylserine, inhibiting thrombin or blocking PAR1 receptor, largely prevented synchrony without blocking ballooning itself. In contrast, inhibition of connexins, P2Y12, P2Y1 or thromboxane formation had no effect on synchrony or ballooning. Importantly, synchronised ballooning was closely followed by a surge in microvesicle formation, which was absent when synchrony was blocked. Our data demonstrate that the mechanism underlying synchronised membrane ballooning requires thrombin generation acting effectively in a positive feedback loop, mediating a subsequent surge in procoagulant activity and microvesicle release.
format	article
author	Ejaife O. Agbani Christopher M. Williams Ingeborg Hers Alastair W. Poole
author_facet	Ejaife O. Agbani Christopher M. Williams Ingeborg Hers Alastair W. Poole
author_sort	Ejaife O. Agbani
title	Membrane Ballooning in Aggregated Platelets is Synchronised and Mediates a Surge in Microvesiculation
title_short	Membrane Ballooning in Aggregated Platelets is Synchronised and Mediates a Surge in Microvesiculation
title_full	Membrane Ballooning in Aggregated Platelets is Synchronised and Mediates a Surge in Microvesiculation
title_fullStr	Membrane Ballooning in Aggregated Platelets is Synchronised and Mediates a Surge in Microvesiculation
title_full_unstemmed	Membrane Ballooning in Aggregated Platelets is Synchronised and Mediates a Surge in Microvesiculation
title_sort	membrane ballooning in aggregated platelets is synchronised and mediates a surge in microvesiculation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/687bb9ef88c64ed9a873232060e73ace
work_keys_str_mv	AT ejaifeoagbani membraneballooninginaggregatedplateletsissynchronisedandmediatesasurgeinmicrovesiculation AT christophermwilliams membraneballooninginaggregatedplateletsissynchronisedandmediatesasurgeinmicrovesiculation AT ingeborghers membraneballooninginaggregatedplateletsissynchronisedandmediatesasurgeinmicrovesiculation AT alastairwpoole membraneballooninginaggregatedplateletsissynchronisedandmediatesasurgeinmicrovesiculation
_version_	1718384964964188160

Membrane Ballooning in Aggregated Platelets is Synchronised and Mediates a Surge in Microvesiculation

Documents similaires