Deficiency of COX7RP, a mitochondrial supercomplex assembly promoting factor, lowers blood glucose level in mice

Abstract Mitochondria are essential organelles to efficiently produce ATP by ATP-synthase, which uses a proton-gradient generated by respiratory chain complexes. We previously demonstrated that COX7RP/COX7A2L/SCAF1 is a key molecule that promotes respiratory supercomplex assembly and regulates energ...

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Autores principales:	Sachiko Shiba, Kazuhiro Ikeda, Kuniko Horie-Inoue, Akitoshi Nakayama, Tomoaki Tanaka, Satoshi Inoue
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/687ef66fb970407391dda94786d7cd4e
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spelling	oai:doaj.org-article:687ef66fb970407391dda94786d7cd4e2021-12-02T12:30:13ZDeficiency of COX7RP, a mitochondrial supercomplex assembly promoting factor, lowers blood glucose level in mice10.1038/s41598-017-08081-z2045-2322https://doaj.org/article/687ef66fb970407391dda94786d7cd4e2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08081-zhttps://doaj.org/toc/2045-2322Abstract Mitochondria are essential organelles to efficiently produce ATP by ATP-synthase, which uses a proton-gradient generated by respiratory chain complexes. We previously demonstrated that COX7RP/COX7A2L/SCAF1 is a key molecule that promotes respiratory supercomplex assembly and regulates energy generation. The contribution of COX7RP to metabolic homeostasis, however, remains to be clarified. In the present study, we showed a metabolic phenotype of Cox7rp knockout (Cox7rpKO) mice, which exhibit lower blood glucose levels after insulin or pyruvate injection. Notably, ATP synthesis rate was reduced in Cox7rpKO mice liver, in accordance with decreased percentages of complex III subunit RISP and complex IV subunit COX1 involved in I + III + IV supercomplex fraction. The present findings suggest that COX7RP-mediated mitochondrial respiration plays crucial roles in the regulation of glucose homeostasis and its impairment will lead to the pathophysiology of metabolic states.Sachiko ShibaKazuhiro IkedaKuniko Horie-InoueAkitoshi NakayamaTomoaki TanakaSatoshi InoueNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sachiko Shiba Kazuhiro Ikeda Kuniko Horie-Inoue Akitoshi Nakayama Tomoaki Tanaka Satoshi Inoue Deficiency of COX7RP, a mitochondrial supercomplex assembly promoting factor, lowers blood glucose level in mice
description	Abstract Mitochondria are essential organelles to efficiently produce ATP by ATP-synthase, which uses a proton-gradient generated by respiratory chain complexes. We previously demonstrated that COX7RP/COX7A2L/SCAF1 is a key molecule that promotes respiratory supercomplex assembly and regulates energy generation. The contribution of COX7RP to metabolic homeostasis, however, remains to be clarified. In the present study, we showed a metabolic phenotype of Cox7rp knockout (Cox7rpKO) mice, which exhibit lower blood glucose levels after insulin or pyruvate injection. Notably, ATP synthesis rate was reduced in Cox7rpKO mice liver, in accordance with decreased percentages of complex III subunit RISP and complex IV subunit COX1 involved in I + III + IV supercomplex fraction. The present findings suggest that COX7RP-mediated mitochondrial respiration plays crucial roles in the regulation of glucose homeostasis and its impairment will lead to the pathophysiology of metabolic states.
format	article
author	Sachiko Shiba Kazuhiro Ikeda Kuniko Horie-Inoue Akitoshi Nakayama Tomoaki Tanaka Satoshi Inoue
author_facet	Sachiko Shiba Kazuhiro Ikeda Kuniko Horie-Inoue Akitoshi Nakayama Tomoaki Tanaka Satoshi Inoue
author_sort	Sachiko Shiba
title	Deficiency of COX7RP, a mitochondrial supercomplex assembly promoting factor, lowers blood glucose level in mice
title_short	Deficiency of COX7RP, a mitochondrial supercomplex assembly promoting factor, lowers blood glucose level in mice
title_full	Deficiency of COX7RP, a mitochondrial supercomplex assembly promoting factor, lowers blood glucose level in mice
title_fullStr	Deficiency of COX7RP, a mitochondrial supercomplex assembly promoting factor, lowers blood glucose level in mice
title_full_unstemmed	Deficiency of COX7RP, a mitochondrial supercomplex assembly promoting factor, lowers blood glucose level in mice
title_sort	deficiency of cox7rp, a mitochondrial supercomplex assembly promoting factor, lowers blood glucose level in mice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/687ef66fb970407391dda94786d7cd4e
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Deficiency of COX7RP, a mitochondrial supercomplex assembly promoting factor, lowers blood glucose level in mice

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