Bacteria Isolated From Milk of Dairy Cows With and Without Clinical Mastitis in Different Regions of Australia and Their AMR Profiles

Mastitis is the most common disease in dairy cattle worldwide. The objectives of this study were to estimate the prevalence of different bacterial species associated with mastitis from dairy herds located in geographically and climatically distinct zones in Australia, and to evaluate the antimicrobi...

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Autores principales:	Hulayyil Al-harbi, Shahab Ranjbar, Robert J. Moore, John I. Alawneh
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	mastitis Staphylococcus Streptococcus Enterobacteriaceae cattle antimicrobial susceptibility Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/687ffccca46c484ba52fa28fcb7de1ad
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spelling	oai:doaj.org-article:687ffccca46c484ba52fa28fcb7de1ad2021-11-04T09:33:04ZBacteria Isolated From Milk of Dairy Cows With and Without Clinical Mastitis in Different Regions of Australia and Their AMR Profiles2297-176910.3389/fvets.2021.743725https://doaj.org/article/687ffccca46c484ba52fa28fcb7de1ad2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.743725/fullhttps://doaj.org/toc/2297-1769Mastitis is the most common disease in dairy cattle worldwide. The objectives of this study were to estimate the prevalence of different bacterial species associated with mastitis from dairy herds located in geographically and climatically distinct zones in Australia, and to evaluate the antimicrobial susceptibility of the isolated bacteria. Quarter-level milk samples (n = 419) were collected from 151 mastitis cases and 268 healthy controls originating from 18 dairy herds located in tropical (Northern Queensland), subtropical (Southeast Queensland) and temperate zones (Victoria) between March and June 2019. Milk samples were cultured, and the isolated bacteria were grouped into six groups: Enterobacteriaceae spp.; Streptococcus spp.; Staphylococcus aureus, non-aureus staphylococci (NAS); Bacillus spp.; and Others. Mixed effects conditional logistic regression models were applied to quantify the association between the prevalence of each bacterial group and the herd zone and bulk milk tank somatic cell counts (BMTSCC). Of the 205 isolates, 102 (50%) originated from mastitis cases, and 103 (50%) from controls. Staphylococci were the most prevalent (NAS 32% and S. aureus 11%). Contagious mastitis bacteria were more prevalent in Victoria compared to Queensland dairy herds. NAS species (P < 0.001) were less prevalent in herds with BMTSCC >300,000 cells/mL compared with herds with low BMTSCC ≤150,000 cells/mL. Enterobacteriaceae and Streptococcus spp. groups showed high resistance rates to 1 (51 and 47%, respectively), and 2 (11 and 23%, respectively), antimicrobials. More than one third of the Enterobacteriaceae (48%) and Others (43%) groups spp. were resistant to at least three antimicrobials. This study provided a unique opportunity to investigate the prevalence of mastitis-associated bacteria in clinical cases and in apparently healthy controls. The findings of this study help inform mastitis control and antimicrobial stewardship programs aimed to reduce the prevalence of mastitis and antimicrobial resistance in dairy herds.Hulayyil Al-harbiHulayyil Al-harbiShahab RanjbarRobert J. MooreJohn I. AlawnehJohn I. AlawnehFrontiers Media S.A.articlemastitisStaphylococcusStreptococcusEnterobacteriaceaecattleantimicrobial susceptibilityVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mastitis Staphylococcus Streptococcus Enterobacteriaceae cattle antimicrobial susceptibility Veterinary medicine SF600-1100
spellingShingle	mastitis Staphylococcus Streptococcus Enterobacteriaceae cattle antimicrobial susceptibility Veterinary medicine SF600-1100 Hulayyil Al-harbi Hulayyil Al-harbi Shahab Ranjbar Robert J. Moore John I. Alawneh John I. Alawneh Bacteria Isolated From Milk of Dairy Cows With and Without Clinical Mastitis in Different Regions of Australia and Their AMR Profiles
description	Mastitis is the most common disease in dairy cattle worldwide. The objectives of this study were to estimate the prevalence of different bacterial species associated with mastitis from dairy herds located in geographically and climatically distinct zones in Australia, and to evaluate the antimicrobial susceptibility of the isolated bacteria. Quarter-level milk samples (n = 419) were collected from 151 mastitis cases and 268 healthy controls originating from 18 dairy herds located in tropical (Northern Queensland), subtropical (Southeast Queensland) and temperate zones (Victoria) between March and June 2019. Milk samples were cultured, and the isolated bacteria were grouped into six groups: Enterobacteriaceae spp.; Streptococcus spp.; Staphylococcus aureus, non-aureus staphylococci (NAS); Bacillus spp.; and Others. Mixed effects conditional logistic regression models were applied to quantify the association between the prevalence of each bacterial group and the herd zone and bulk milk tank somatic cell counts (BMTSCC). Of the 205 isolates, 102 (50%) originated from mastitis cases, and 103 (50%) from controls. Staphylococci were the most prevalent (NAS 32% and S. aureus 11%). Contagious mastitis bacteria were more prevalent in Victoria compared to Queensland dairy herds. NAS species (P < 0.001) were less prevalent in herds with BMTSCC >300,000 cells/mL compared with herds with low BMTSCC ≤150,000 cells/mL. Enterobacteriaceae and Streptococcus spp. groups showed high resistance rates to 1 (51 and 47%, respectively), and 2 (11 and 23%, respectively), antimicrobials. More than one third of the Enterobacteriaceae (48%) and Others (43%) groups spp. were resistant to at least three antimicrobials. This study provided a unique opportunity to investigate the prevalence of mastitis-associated bacteria in clinical cases and in apparently healthy controls. The findings of this study help inform mastitis control and antimicrobial stewardship programs aimed to reduce the prevalence of mastitis and antimicrobial resistance in dairy herds.
format	article
author	Hulayyil Al-harbi Hulayyil Al-harbi Shahab Ranjbar Robert J. Moore John I. Alawneh John I. Alawneh
author_facet	Hulayyil Al-harbi Hulayyil Al-harbi Shahab Ranjbar Robert J. Moore John I. Alawneh John I. Alawneh
author_sort	Hulayyil Al-harbi
title	Bacteria Isolated From Milk of Dairy Cows With and Without Clinical Mastitis in Different Regions of Australia and Their AMR Profiles
title_short	Bacteria Isolated From Milk of Dairy Cows With and Without Clinical Mastitis in Different Regions of Australia and Their AMR Profiles
title_full	Bacteria Isolated From Milk of Dairy Cows With and Without Clinical Mastitis in Different Regions of Australia and Their AMR Profiles
title_fullStr	Bacteria Isolated From Milk of Dairy Cows With and Without Clinical Mastitis in Different Regions of Australia and Their AMR Profiles
title_full_unstemmed	Bacteria Isolated From Milk of Dairy Cows With and Without Clinical Mastitis in Different Regions of Australia and Their AMR Profiles
title_sort	bacteria isolated from milk of dairy cows with and without clinical mastitis in different regions of australia and their amr profiles
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/687ffccca46c484ba52fa28fcb7de1ad
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Bacteria Isolated From Milk of Dairy Cows With and Without Clinical Mastitis in Different Regions of Australia and Their AMR Profiles

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