A Novel Glycolipid Biosurfactant Confers Grazing Resistance upon <named-content content-type="genus-species">Pantoea ananatis</named-content> BRT175 against the Social Amoeba <named-content content-type="genus-species">Dictyostelium discoideum</named-content>

ABSTRACT Pantoea is a versatile genus of bacteria with both plant- and animal-pathogenic strains, some of which have been suggested to cause human infections. There is, however, limited knowledge on the potential determinants used for host association and pathogenesis in animal systems. In this stud...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Derek D. N. Smith, Arvin Nickzad, Eric Déziel, John Stavrinides
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	biosurfactants Pantoea Dictyostelium opportunistic infections Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/6888926e78ae449f9b13ffd71be1d49c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6888926e78ae449f9b13ffd71be1d49c
record_format	dspace
spelling	oai:doaj.org-article:6888926e78ae449f9b13ffd71be1d49c2021-11-15T15:21:38ZA Novel Glycolipid Biosurfactant Confers Grazing Resistance upon <named-content content-type="genus-species">Pantoea ananatis</named-content> BRT175 against the Social Amoeba <named-content content-type="genus-species">Dictyostelium discoideum</named-content>10.1128/mSphere.00075-152379-5042https://doaj.org/article/6888926e78ae449f9b13ffd71be1d49c2016-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00075-15https://doaj.org/toc/2379-5042ABSTRACT Pantoea is a versatile genus of bacteria with both plant- and animal-pathogenic strains, some of which have been suggested to cause human infections. There is, however, limited knowledge on the potential determinants used for host association and pathogenesis in animal systems. In this study, we used the model host Dictyostelium discoideum to show that isolates of Pantoea ananatis exhibit differential grazing susceptibility, with some being resistant to grazing by the amoebae. We carried out a high-throughput genetic screen of one grazing-resistant isolate, P. ananatis BRT175, using the D. discoideum pathosystem to identify genes responsible for the resistance phenotype. Among the 26 candidate genes involved in grazing resistance, we identified rhlA and rhlB, which we show are involved in the biosynthesis of a biosurfactant that enables swarming motility in P. ananatis BRT175. Using liquid chromatography-mass spectrometry (LC-MS), the biosurfactant was shown to be a glycolipid with monohexose-C10-C10 as the primary congener. We show that this novel glycolipid biosurfactant is cytotoxic to the amoebae and is capable of compromising cellular integrity, leading to cell lysis. The production of this biosurfactant may be important for bacterial survival in the environment and could contribute to the establishment of opportunistic infections. IMPORTANCE The genetic factors used for host interaction by the opportunistic human pathogen Pantoea ananatis are largely unknown. We identified two genes that are important for the production of a biosurfactant that confers grazing resistance against the social amoeba Dictyostelium discoideum. We show that the biosurfactant, which exhibits cytotoxicity toward the amoebae, is a glycolipid that incorporates a hexose rather than rhamnose. The production of this biosurfactant may confer a competitive advantage in the environment and could potentially contribute to the establishment of opportunistic infections.Derek D. N. SmithArvin NickzadEric DézielJohn StavrinidesAmerican Society for MicrobiologyarticlebiosurfactantsPantoeaDictyosteliumopportunistic infectionsMicrobiologyQR1-502ENmSphere, Vol 1, Iss 1 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	biosurfactants Pantoea Dictyostelium opportunistic infections Microbiology QR1-502
spellingShingle	biosurfactants Pantoea Dictyostelium opportunistic infections Microbiology QR1-502 Derek D. N. Smith Arvin Nickzad Eric Déziel John Stavrinides A Novel Glycolipid Biosurfactant Confers Grazing Resistance upon <named-content content-type="genus-species">Pantoea ananatis</named-content> BRT175 against the Social Amoeba <named-content content-type="genus-species">Dictyostelium discoideum</named-content>
description	ABSTRACT Pantoea is a versatile genus of bacteria with both plant- and animal-pathogenic strains, some of which have been suggested to cause human infections. There is, however, limited knowledge on the potential determinants used for host association and pathogenesis in animal systems. In this study, we used the model host Dictyostelium discoideum to show that isolates of Pantoea ananatis exhibit differential grazing susceptibility, with some being resistant to grazing by the amoebae. We carried out a high-throughput genetic screen of one grazing-resistant isolate, P. ananatis BRT175, using the D. discoideum pathosystem to identify genes responsible for the resistance phenotype. Among the 26 candidate genes involved in grazing resistance, we identified rhlA and rhlB, which we show are involved in the biosynthesis of a biosurfactant that enables swarming motility in P. ananatis BRT175. Using liquid chromatography-mass spectrometry (LC-MS), the biosurfactant was shown to be a glycolipid with monohexose-C10-C10 as the primary congener. We show that this novel glycolipid biosurfactant is cytotoxic to the amoebae and is capable of compromising cellular integrity, leading to cell lysis. The production of this biosurfactant may be important for bacterial survival in the environment and could contribute to the establishment of opportunistic infections. IMPORTANCE The genetic factors used for host interaction by the opportunistic human pathogen Pantoea ananatis are largely unknown. We identified two genes that are important for the production of a biosurfactant that confers grazing resistance against the social amoeba Dictyostelium discoideum. We show that the biosurfactant, which exhibits cytotoxicity toward the amoebae, is a glycolipid that incorporates a hexose rather than rhamnose. The production of this biosurfactant may confer a competitive advantage in the environment and could potentially contribute to the establishment of opportunistic infections.
format	article
author	Derek D. N. Smith Arvin Nickzad Eric Déziel John Stavrinides
author_facet	Derek D. N. Smith Arvin Nickzad Eric Déziel John Stavrinides
author_sort	Derek D. N. Smith
title	A Novel Glycolipid Biosurfactant Confers Grazing Resistance upon <named-content content-type="genus-species">Pantoea ananatis</named-content> BRT175 against the Social Amoeba <named-content content-type="genus-species">Dictyostelium discoideum</named-content>
title_short	A Novel Glycolipid Biosurfactant Confers Grazing Resistance upon <named-content content-type="genus-species">Pantoea ananatis</named-content> BRT175 against the Social Amoeba <named-content content-type="genus-species">Dictyostelium discoideum</named-content>
title_full	A Novel Glycolipid Biosurfactant Confers Grazing Resistance upon <named-content content-type="genus-species">Pantoea ananatis</named-content> BRT175 against the Social Amoeba <named-content content-type="genus-species">Dictyostelium discoideum</named-content>
title_fullStr	A Novel Glycolipid Biosurfactant Confers Grazing Resistance upon <named-content content-type="genus-species">Pantoea ananatis</named-content> BRT175 against the Social Amoeba <named-content content-type="genus-species">Dictyostelium discoideum</named-content>
title_full_unstemmed	A Novel Glycolipid Biosurfactant Confers Grazing Resistance upon <named-content content-type="genus-species">Pantoea ananatis</named-content> BRT175 against the Social Amoeba <named-content content-type="genus-species">Dictyostelium discoideum</named-content>
title_sort	novel glycolipid biosurfactant confers grazing resistance upon <named-content content-type="genus-species">pantoea ananatis</named-content> brt175 against the social amoeba <named-content content-type="genus-species">dictyostelium discoideum</named-content>
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/6888926e78ae449f9b13ffd71be1d49c
work_keys_str_mv	AT derekdnsmith anovelglycolipidbiosurfactantconfersgrazingresistanceuponnamedcontentcontenttypegenusspeciespantoeaananatisnamedcontentbrt175againstthesocialamoebanamedcontentcontenttypegenusspeciesdictyosteliumdiscoideumnamedcontent AT arvinnickzad anovelglycolipidbiosurfactantconfersgrazingresistanceuponnamedcontentcontenttypegenusspeciespantoeaananatisnamedcontentbrt175againstthesocialamoebanamedcontentcontenttypegenusspeciesdictyosteliumdiscoideumnamedcontent AT ericdeziel anovelglycolipidbiosurfactantconfersgrazingresistanceuponnamedcontentcontenttypegenusspeciespantoeaananatisnamedcontentbrt175againstthesocialamoebanamedcontentcontenttypegenusspeciesdictyosteliumdiscoideumnamedcontent AT johnstavrinides anovelglycolipidbiosurfactantconfersgrazingresistanceuponnamedcontentcontenttypegenusspeciespantoeaananatisnamedcontentbrt175againstthesocialamoebanamedcontentcontenttypegenusspeciesdictyosteliumdiscoideumnamedcontent AT derekdnsmith novelglycolipidbiosurfactantconfersgrazingresistanceuponnamedcontentcontenttypegenusspeciespantoeaananatisnamedcontentbrt175againstthesocialamoebanamedcontentcontenttypegenusspeciesdictyosteliumdiscoideumnamedcontent AT arvinnickzad novelglycolipidbiosurfactantconfersgrazingresistanceuponnamedcontentcontenttypegenusspeciespantoeaananatisnamedcontentbrt175againstthesocialamoebanamedcontentcontenttypegenusspeciesdictyosteliumdiscoideumnamedcontent AT ericdeziel novelglycolipidbiosurfactantconfersgrazingresistanceuponnamedcontentcontenttypegenusspeciespantoeaananatisnamedcontentbrt175againstthesocialamoebanamedcontentcontenttypegenusspeciesdictyosteliumdiscoideumnamedcontent AT johnstavrinides novelglycolipidbiosurfactantconfersgrazingresistanceuponnamedcontentcontenttypegenusspeciespantoeaananatisnamedcontentbrt175againstthesocialamoebanamedcontentcontenttypegenusspeciesdictyosteliumdiscoideumnamedcontent
_version_	1718428132792336384

A Novel Glycolipid Biosurfactant Confers Grazing Resistance upon <named-content content-type="genus-species">Pantoea ananatis</named-content> BRT175 against the Social Amoeba <named-content content-type="genus-species">Dictyostelium discoideum</named-content>

Ejemplares similares