GntR-like SCO3932 Protein Provides a Link between Actinomycete Integrative and Conjugative Elements and Secondary Metabolism

<i>Streptomyces</i> bacteria produce a plethora of secondary metabolites including the majority of medically important antibiotics. The onset of secondary metabolism is correlated with morphological differentiation and controlled by a complex regulatory network involving numerous regulat...

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Autores principales:	Krzysztof J. Pawlik, Mateusz Zelkowski, Mateusz Biernacki, Katarzyna Litwinska, Pawel Jaworski, Magdalena Kotowska
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	streptomyces specialized metabolism regulation AICEs GntR HutC KorSA Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/688a78dd1fa849bfa29eac95b60763e2
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spelling	oai:doaj.org-article:688a78dd1fa849bfa29eac95b60763e22021-11-11T17:17:24ZGntR-like SCO3932 Protein Provides a Link between Actinomycete Integrative and Conjugative Elements and Secondary Metabolism10.3390/ijms2221118671422-00671661-6596https://doaj.org/article/688a78dd1fa849bfa29eac95b60763e22021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11867https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067<i>Streptomyces</i> bacteria produce a plethora of secondary metabolites including the majority of medically important antibiotics. The onset of secondary metabolism is correlated with morphological differentiation and controlled by a complex regulatory network involving numerous regulatory proteins. Control over these pathways at the molecular level has a medical and industrial importance. Here we describe a GntR-like DNA binding transcription factor SCO3932, encoded within an actinomycete integrative and conjugative element, which is involved in the secondary metabolite biosynthesis regulation. Affinity chromatography, electrophoresis mobility shift assay, footprinting and chromatin immunoprecipitation experiments revealed, both in vitro and in vivo, SCO3932 binding capability to its own promoter region shared with the neighboring gene <i>SCO3933</i>, as well as promoters of polyketide metabolite genes, such as <i>cpkD</i>, a coelimycin biosynthetic gene, and <i>actII-orf4</i>—an activator of actinorhodin biosynthesis. Increased activity of SCO3932 target promoters, as a result of SCO3932 overproduction, indicates an activatory role of this protein in <i>Streptomyces coelicolor</i> A3(2) metabolite synthesis pathways.Krzysztof J. PawlikMateusz ZelkowskiMateusz BiernackiKatarzyna LitwinskaPawel JaworskiMagdalena KotowskaMDPI AGarticlestreptomycesspecialized metabolism regulationAICEsGntRHutCKorSABiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11867, p 11867 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	streptomyces specialized metabolism regulation AICEs GntR HutC KorSA Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	streptomyces specialized metabolism regulation AICEs GntR HutC KorSA Biology (General) QH301-705.5 Chemistry QD1-999 Krzysztof J. Pawlik Mateusz Zelkowski Mateusz Biernacki Katarzyna Litwinska Pawel Jaworski Magdalena Kotowska GntR-like SCO3932 Protein Provides a Link between Actinomycete Integrative and Conjugative Elements and Secondary Metabolism
description	<i>Streptomyces</i> bacteria produce a plethora of secondary metabolites including the majority of medically important antibiotics. The onset of secondary metabolism is correlated with morphological differentiation and controlled by a complex regulatory network involving numerous regulatory proteins. Control over these pathways at the molecular level has a medical and industrial importance. Here we describe a GntR-like DNA binding transcription factor SCO3932, encoded within an actinomycete integrative and conjugative element, which is involved in the secondary metabolite biosynthesis regulation. Affinity chromatography, electrophoresis mobility shift assay, footprinting and chromatin immunoprecipitation experiments revealed, both in vitro and in vivo, SCO3932 binding capability to its own promoter region shared with the neighboring gene <i>SCO3933</i>, as well as promoters of polyketide metabolite genes, such as <i>cpkD</i>, a coelimycin biosynthetic gene, and <i>actII-orf4</i>—an activator of actinorhodin biosynthesis. Increased activity of SCO3932 target promoters, as a result of SCO3932 overproduction, indicates an activatory role of this protein in <i>Streptomyces coelicolor</i> A3(2) metabolite synthesis pathways.
format	article
author	Krzysztof J. Pawlik Mateusz Zelkowski Mateusz Biernacki Katarzyna Litwinska Pawel Jaworski Magdalena Kotowska
author_facet	Krzysztof J. Pawlik Mateusz Zelkowski Mateusz Biernacki Katarzyna Litwinska Pawel Jaworski Magdalena Kotowska
author_sort	Krzysztof J. Pawlik
title	GntR-like SCO3932 Protein Provides a Link between Actinomycete Integrative and Conjugative Elements and Secondary Metabolism
title_short	GntR-like SCO3932 Protein Provides a Link between Actinomycete Integrative and Conjugative Elements and Secondary Metabolism
title_full	GntR-like SCO3932 Protein Provides a Link between Actinomycete Integrative and Conjugative Elements and Secondary Metabolism
title_fullStr	GntR-like SCO3932 Protein Provides a Link between Actinomycete Integrative and Conjugative Elements and Secondary Metabolism
title_full_unstemmed	GntR-like SCO3932 Protein Provides a Link between Actinomycete Integrative and Conjugative Elements and Secondary Metabolism
title_sort	gntr-like sco3932 protein provides a link between actinomycete integrative and conjugative elements and secondary metabolism
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/688a78dd1fa849bfa29eac95b60763e2
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GntR-like SCO3932 Protein Provides a Link between Actinomycete Integrative and Conjugative Elements and Secondary Metabolism

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