A flat embedding method for transmission electron microscopy reveals an unknown mechanism of tetracycline

Wenzel et al. developed a flat embedding method for TEM, which increases the fraction of appropriately orientated cells per image, making it easier to quantify cell morphology. They find that tetracycline-treated B. subtilis shows membrane lesions and that tetracycline action is via direct perturbat...

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Autores principales: Michaela Wenzel, Marien P. Dekker, Biwen Wang, Maroeska J. Burggraaf, Wilbert Bitter, Jan R. T. van Weering, Leendert W. Hamoen
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/6891a8b700bc4b0d86ca8c303d7203c1
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Sumario:Wenzel et al. developed a flat embedding method for TEM, which increases the fraction of appropriately orientated cells per image, making it easier to quantify cell morphology. They find that tetracycline-treated B. subtilis shows membrane lesions and that tetracycline action is via direct perturbation of bacterial cell membrane in addition to its known ribosome inhibition activity.