Fibronectin-coating enhances attachment and proliferation of mesenchymal stem cells on a polyurethane meniscal scaffold

Introduction: Partial meniscectomy is one of the most common surgical strategy for a meniscal injury, but sometimes, patients complain of knee pain due to an overload in the ablated compartment. In these cases, implantation of tissue engineering scaffold could be indicated. Currently, two commercial...

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Autores principales:	Raquel Arredondo, Francesco Poggioli, Santos Martínez-Díaz, María Piera-Trilla, Raúl Torres-Claramunt, Laura Tío, Joan C. Monllau
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Meniscal injuries Post-meniscectomy syndrome Tissue engineering Scaffolds Fibronectin Mesenchymal stem cell Medicine (General) R5-920 Cytology QH573-671
Acceso en línea:	https://doaj.org/article/6897949541564cc2b93a9d3a537485f3
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spelling	oai:doaj.org-article:6897949541564cc2b93a9d3a537485f32021-11-28T04:33:19ZFibronectin-coating enhances attachment and proliferation of mesenchymal stem cells on a polyurethane meniscal scaffold2352-320410.1016/j.reth.2021.11.001https://doaj.org/article/6897949541564cc2b93a9d3a537485f32021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2352320421000833https://doaj.org/toc/2352-3204Introduction: Partial meniscectomy is one of the most common surgical strategy for a meniscal injury, but sometimes, patients complain of knee pain due to an overload in the ablated compartment. In these cases, implantation of tissue engineering scaffold could be indicated. Currently, two commercial scaffolds, based on collagen or polycaprolactone-polyurethane (PCL-PU), are available for meniscus scaffolding. In short term follow-up assessments, both showed clinical improvement and tissue formation. However, long-term studies carried out in PCL-PU showed that the new tissue decreased in volume and assumed an irregular shape. Moreover, in some cases, the scaffold was totally reabsorbed, without new tissue formation.Mesenchymal stem cells (MSCs) combined with scaffolds could represents a promising approach for treating meniscal defects because of their multipotency and self-renewal. In this work, we aimed to compare the behaviour of MSCs and chondrocytes on a PCL-PU scaffold in vitro. MSCs express integrins that binds to fibronectin (FN), so we also investigate the effect of a FN coating on the bioactivity of the scaffold. Methods: We isolated rabbit bone marrow MSCs (rBM-MSCs) from two skeletally mature New Zealand white rabbits and stablished the optimum culture condition to expand them. Then, they were seeded over non-coated and FN-coated scaffolds and cultured in chondrogenic conditions. To evaluate cell functionality, we performed an MTS assay to compare cell proliferation between both conditions. Finally, a histologic study was performed to assess extracellular matrix (ECM) production in both samples, and to compare them with the ones obtained with rabbit chondrocytes (rCHs) seeded in a non-coated scaffold. Results: A culture protocol based on low FBS concentration was set as the best for rBM-MSCs expansion. The MTS assay revealed that rBM-MSCs seeded on FN-coated scaffolds have more cells on proliferation (145%; 95% CI: 107%–182%) compared with rBM-MSCs seeded on non-coated scaffolds. Finally, the histologic study demonstrated that rCHs seeded on non-coated scaffolds displayed the highest production of ECM, followed by rBM-MSCs seeded on FN-coated scaffolds. Furthermore, both cell types produced a comparable ECM pattern. Conclusion: These results suggest that MSCs have low capacity attachment to PCL-PU scaffolds, but the presence of integrin alpha5beta1 (FN-receptor) in MSCs allows them to interact with the FN-coated scaffolds. These results could be applied in the design of scaffolds, and might have important clinical implications in orthopaedic surgery of meniscal injuries.Raquel ArredondoFrancesco PoggioliSantos Martínez-DíazMaría Piera-TrillaRaúl Torres-ClaramuntLaura TíoJoan C. MonllauElsevierarticleMeniscal injuriesPost-meniscectomy syndromeTissue engineeringScaffoldsFibronectinMesenchymal stem cellMedicine (General)R5-920CytologyQH573-671ENRegenerative Therapy, Vol 18, Iss , Pp 480-486 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Meniscal injuries Post-meniscectomy syndrome Tissue engineering Scaffolds Fibronectin Mesenchymal stem cell Medicine (General) R5-920 Cytology QH573-671
spellingShingle	Meniscal injuries Post-meniscectomy syndrome Tissue engineering Scaffolds Fibronectin Mesenchymal stem cell Medicine (General) R5-920 Cytology QH573-671 Raquel Arredondo Francesco Poggioli Santos Martínez-Díaz María Piera-Trilla Raúl Torres-Claramunt Laura Tío Joan C. Monllau Fibronectin-coating enhances attachment and proliferation of mesenchymal stem cells on a polyurethane meniscal scaffold
description	Introduction: Partial meniscectomy is one of the most common surgical strategy for a meniscal injury, but sometimes, patients complain of knee pain due to an overload in the ablated compartment. In these cases, implantation of tissue engineering scaffold could be indicated. Currently, two commercial scaffolds, based on collagen or polycaprolactone-polyurethane (PCL-PU), are available for meniscus scaffolding. In short term follow-up assessments, both showed clinical improvement and tissue formation. However, long-term studies carried out in PCL-PU showed that the new tissue decreased in volume and assumed an irregular shape. Moreover, in some cases, the scaffold was totally reabsorbed, without new tissue formation.Mesenchymal stem cells (MSCs) combined with scaffolds could represents a promising approach for treating meniscal defects because of their multipotency and self-renewal. In this work, we aimed to compare the behaviour of MSCs and chondrocytes on a PCL-PU scaffold in vitro. MSCs express integrins that binds to fibronectin (FN), so we also investigate the effect of a FN coating on the bioactivity of the scaffold. Methods: We isolated rabbit bone marrow MSCs (rBM-MSCs) from two skeletally mature New Zealand white rabbits and stablished the optimum culture condition to expand them. Then, they were seeded over non-coated and FN-coated scaffolds and cultured in chondrogenic conditions. To evaluate cell functionality, we performed an MTS assay to compare cell proliferation between both conditions. Finally, a histologic study was performed to assess extracellular matrix (ECM) production in both samples, and to compare them with the ones obtained with rabbit chondrocytes (rCHs) seeded in a non-coated scaffold. Results: A culture protocol based on low FBS concentration was set as the best for rBM-MSCs expansion. The MTS assay revealed that rBM-MSCs seeded on FN-coated scaffolds have more cells on proliferation (145%; 95% CI: 107%–182%) compared with rBM-MSCs seeded on non-coated scaffolds. Finally, the histologic study demonstrated that rCHs seeded on non-coated scaffolds displayed the highest production of ECM, followed by rBM-MSCs seeded on FN-coated scaffolds. Furthermore, both cell types produced a comparable ECM pattern. Conclusion: These results suggest that MSCs have low capacity attachment to PCL-PU scaffolds, but the presence of integrin alpha5beta1 (FN-receptor) in MSCs allows them to interact with the FN-coated scaffolds. These results could be applied in the design of scaffolds, and might have important clinical implications in orthopaedic surgery of meniscal injuries.
format	article
author	Raquel Arredondo Francesco Poggioli Santos Martínez-Díaz María Piera-Trilla Raúl Torres-Claramunt Laura Tío Joan C. Monllau
author_facet	Raquel Arredondo Francesco Poggioli Santos Martínez-Díaz María Piera-Trilla Raúl Torres-Claramunt Laura Tío Joan C. Monllau
author_sort	Raquel Arredondo
title	Fibronectin-coating enhances attachment and proliferation of mesenchymal stem cells on a polyurethane meniscal scaffold
title_short	Fibronectin-coating enhances attachment and proliferation of mesenchymal stem cells on a polyurethane meniscal scaffold
title_full	Fibronectin-coating enhances attachment and proliferation of mesenchymal stem cells on a polyurethane meniscal scaffold
title_fullStr	Fibronectin-coating enhances attachment and proliferation of mesenchymal stem cells on a polyurethane meniscal scaffold
title_full_unstemmed	Fibronectin-coating enhances attachment and proliferation of mesenchymal stem cells on a polyurethane meniscal scaffold
title_sort	fibronectin-coating enhances attachment and proliferation of mesenchymal stem cells on a polyurethane meniscal scaffold
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/6897949541564cc2b93a9d3a537485f3
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Fibronectin-coating enhances attachment and proliferation of mesenchymal stem cells on a polyurethane meniscal scaffold

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