Prevalence and Characteristics of Phenicol-Oxazolidinone Resistance Genes in <i>Enterococcus Faecalis</i> and <i>Enterococcus Faecium</i> Isolated from Food-Producing Animals and Meat in Korea

Prevalence and Characteristics of Phenicol-Oxazolidinone Resistance Genes in <i>Enterococcus Faecalis</i> and <i>Enterococcus Faecium</i> Isolated from Food-Producing Animals and Meat in Korea

The use of phenicol antibiotics in animals has increased. In recent years, it has been reported that the transferable gene mediates phenicol-oxazolidinone resistance. This study analyzed the prevalence and characteristics of phenicol-oxazolidinone resistance genes in <i>Enterococcus faecalis&l...

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Autores principales: Eiseul Kim, So-Won Shin, Hyo-Sun Kwak, Min-Hyeok Cha, Seung-Min Yang, Yoon-Soo Gwak, Gun-Jo Woo, Hae-Yeong Kim
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
<i>Enterococcus</i>
linezolid
antibiotic resistance gene
phenicol-oxazolidinone resistance gene
<i>optrA</i>
<i>fexA</i>
Biology (General)
QH301-705.5
Chemistry
QD1-999
Acceso en línea:https://doaj.org/article/689ac7e3eec94535b0b577ce1a8f79af
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spelling oai:doaj.org-article:689ac7e3eec94535b0b577ce1a8f79af2021-11-11T16:48:53ZPrevalence and Characteristics of Phenicol-Oxazolidinone Resistance Genes in <i>Enterococcus Faecalis</i> and <i>Enterococcus Faecium</i> Isolated from Food-Producing Animals and Meat in Korea10.3390/ijms2221113351422-00671661-6596https://doaj.org/article/689ac7e3eec94535b0b577ce1a8f79af2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11335https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The use of phenicol antibiotics in animals has increased. In recent years, it has been reported that the transferable gene mediates phenicol-oxazolidinone resistance. This study analyzed the prevalence and characteristics of phenicol-oxazolidinone resistance genes in <i>Enterococcus faecalis</i> and <i>Enterococcus faecium</i> isolated from food-producing animals and meat in Korea in 2018. Furthermore, for the first time, we reported the genome sequence of <i>E. faecalis</i> strain, which possesses the phenicol-oxazolidinone resistance gene on both the chromosome and plasmid. Among the 327 isolates, <i>optrA</i>, <i>poxtA</i>, and <i>fexA</i> genes were found in 15 (4.6%), 8 (2.5%), and 17 isolates (5.2%), respectively. Twenty <i>E. faecalis</i> strains carrying resistance genes belonged to eight sequence types (STs), and transferability was found in 17 isolates. The genome sequences revealed that resistant genes were present in the chromosome or plasmid, or both. In strains EFS17 and EFS108, <i>optrA</i> was located downstream of the <i>ermA</i> and <i>ant</i>(<i>9</i>)<i>-1</i> genes. The strains EFS36 and EFS108 harboring <i>poxtA</i>-encoding plasmid cocarried <i>fexA</i> and <i>cfr</i>(<i>D</i>). These islands also contained IS1216E or the transposon Tn554, enabling the horizontal transfer of the phenicol-oxazolidinone resistance with other antimicrobial-resistant genes. Our results suggest that it is necessary to promote the prudent use of antibiotics through continuous monitoring and reevaluation.Eiseul KimSo-Won ShinHyo-Sun KwakMin-Hyeok ChaSeung-Min YangYoon-Soo GwakGun-Jo WooHae-Yeong KimMDPI AGarticle<i>Enterococcus</i>linezolidantibiotic resistance genephenicol-oxazolidinone resistance gene<i>optrA</i><i>fexA</i>Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11335, p 11335 (2021)
institution DOAJ
collection DOAJ
language EN
topic <i>Enterococcus</i>
linezolid
antibiotic resistance gene
phenicol-oxazolidinone resistance gene
<i>optrA</i>
<i>fexA</i>
Biology (General)
QH301-705.5
Chemistry
QD1-999
spellingShingle <i>Enterococcus</i>
linezolid
antibiotic resistance gene
phenicol-oxazolidinone resistance gene
<i>optrA</i>
<i>fexA</i>
Biology (General)
QH301-705.5
Chemistry
QD1-999
Eiseul Kim
So-Won Shin
Hyo-Sun Kwak
Min-Hyeok Cha
Seung-Min Yang
Yoon-Soo Gwak
Gun-Jo Woo
Hae-Yeong Kim
Prevalence and Characteristics of Phenicol-Oxazolidinone Resistance Genes in <i>Enterococcus Faecalis</i> and <i>Enterococcus Faecium</i> Isolated from Food-Producing Animals and Meat in Korea
description The use of phenicol antibiotics in animals has increased. In recent years, it has been reported that the transferable gene mediates phenicol-oxazolidinone resistance. This study analyzed the prevalence and characteristics of phenicol-oxazolidinone resistance genes in <i>Enterococcus faecalis</i> and <i>Enterococcus faecium</i> isolated from food-producing animals and meat in Korea in 2018. Furthermore, for the first time, we reported the genome sequence of <i>E. faecalis</i> strain, which possesses the phenicol-oxazolidinone resistance gene on both the chromosome and plasmid. Among the 327 isolates, <i>optrA</i>, <i>poxtA</i>, and <i>fexA</i> genes were found in 15 (4.6%), 8 (2.5%), and 17 isolates (5.2%), respectively. Twenty <i>E. faecalis</i> strains carrying resistance genes belonged to eight sequence types (STs), and transferability was found in 17 isolates. The genome sequences revealed that resistant genes were present in the chromosome or plasmid, or both. In strains EFS17 and EFS108, <i>optrA</i> was located downstream of the <i>ermA</i> and <i>ant</i>(<i>9</i>)<i>-1</i> genes. The strains EFS36 and EFS108 harboring <i>poxtA</i>-encoding plasmid cocarried <i>fexA</i> and <i>cfr</i>(<i>D</i>). These islands also contained IS1216E or the transposon Tn554, enabling the horizontal transfer of the phenicol-oxazolidinone resistance with other antimicrobial-resistant genes. Our results suggest that it is necessary to promote the prudent use of antibiotics through continuous monitoring and reevaluation.
format article
author Eiseul Kim
So-Won Shin
Hyo-Sun Kwak
Min-Hyeok Cha
Seung-Min Yang
Yoon-Soo Gwak
Gun-Jo Woo
Hae-Yeong Kim
author_facet Eiseul Kim
So-Won Shin
Hyo-Sun Kwak
Min-Hyeok Cha
Seung-Min Yang
Yoon-Soo Gwak
Gun-Jo Woo
Hae-Yeong Kim
author_sort Eiseul Kim
title Prevalence and Characteristics of Phenicol-Oxazolidinone Resistance Genes in <i>Enterococcus Faecalis</i> and <i>Enterococcus Faecium</i> Isolated from Food-Producing Animals and Meat in Korea
title_short Prevalence and Characteristics of Phenicol-Oxazolidinone Resistance Genes in <i>Enterococcus Faecalis</i> and <i>Enterococcus Faecium</i> Isolated from Food-Producing Animals and Meat in Korea
title_full Prevalence and Characteristics of Phenicol-Oxazolidinone Resistance Genes in <i>Enterococcus Faecalis</i> and <i>Enterococcus Faecium</i> Isolated from Food-Producing Animals and Meat in Korea
title_fullStr Prevalence and Characteristics of Phenicol-Oxazolidinone Resistance Genes in <i>Enterococcus Faecalis</i> and <i>Enterococcus Faecium</i> Isolated from Food-Producing Animals and Meat in Korea
title_full_unstemmed Prevalence and Characteristics of Phenicol-Oxazolidinone Resistance Genes in <i>Enterococcus Faecalis</i> and <i>Enterococcus Faecium</i> Isolated from Food-Producing Animals and Meat in Korea
title_sort prevalence and characteristics of phenicol-oxazolidinone resistance genes in <i>enterococcus faecalis</i> and <i>enterococcus faecium</i> isolated from food-producing animals and meat in korea
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/689ac7e3eec94535b0b577ce1a8f79af
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