Burkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties
Abstract Melioidosis, a severe infection with the environmental bacterium Burkholderia pseudomallei, is being recognised increasingly frequently. What determines its uneven distribution within endemic areas is poorly understood. We cultured soil from a rice field in Laos for B. pseudomallei at diffe...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2017
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/689df61bb11a4ffbbc548f4d9702e963 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:689df61bb11a4ffbbc548f4d9702e963 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:689df61bb11a4ffbbc548f4d9702e9632021-12-02T16:05:57ZBurkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties10.1038/s41598-017-02946-z2045-2322https://doaj.org/article/689df61bb11a4ffbbc548f4d9702e9632017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02946-zhttps://doaj.org/toc/2045-2322Abstract Melioidosis, a severe infection with the environmental bacterium Burkholderia pseudomallei, is being recognised increasingly frequently. What determines its uneven distribution within endemic areas is poorly understood. We cultured soil from a rice field in Laos for B. pseudomallei at different depths on 4 occasions over a 13-month period. We also measured physical and chemical parameters in order to identify associated characteristics. Overall, 195 of 653 samples (29.7%) yielded B. pseudomallei. A higher prevalence of B. pseudomallei was found at soil depths greater than the 30 cm currently recommended for B. pseudomallei environmental sampling. B. pseudomallei was associated with a high soil water content and low total nitrogen, carbon and organic matter content. Our results suggested that a sampling grid of 25 five metre square quadrats (i.e. 25 × 25 m) should be sufficient to detect B. pseudomallei at a given location if samples are taken at a soil depth of at least 60 cm. However, culture of B. pseudomallei in environmental samples is difficult and liable to variation. Future studies should both rely on molecular approaches and address the micro-heterogeneity of soil when investigating physico-chemical associations with the presence of B. pseudomallei.L. ManivanhA. PierretS. RattanavongO. KounnavongsaY. BuissonI. ElliottJ. -L. MaeghtK. XayyathipJ. SilisoukM. VongsouvathR. PhetsouvanhP. N. NewtonG. LacombeO. RibolziE. Rochelle-NewallD. A. B. DanceNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q L. Manivanh A. Pierret S. Rattanavong O. Kounnavongsa Y. Buisson I. Elliott J. -L. Maeght K. Xayyathip J. Silisouk M. Vongsouvath R. Phetsouvanh P. N. Newton G. Lacombe O. Ribolzi E. Rochelle-Newall D. A. B. Dance Burkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties |
| description |
Abstract Melioidosis, a severe infection with the environmental bacterium Burkholderia pseudomallei, is being recognised increasingly frequently. What determines its uneven distribution within endemic areas is poorly understood. We cultured soil from a rice field in Laos for B. pseudomallei at different depths on 4 occasions over a 13-month period. We also measured physical and chemical parameters in order to identify associated characteristics. Overall, 195 of 653 samples (29.7%) yielded B. pseudomallei. A higher prevalence of B. pseudomallei was found at soil depths greater than the 30 cm currently recommended for B. pseudomallei environmental sampling. B. pseudomallei was associated with a high soil water content and low total nitrogen, carbon and organic matter content. Our results suggested that a sampling grid of 25 five metre square quadrats (i.e. 25 × 25 m) should be sufficient to detect B. pseudomallei at a given location if samples are taken at a soil depth of at least 60 cm. However, culture of B. pseudomallei in environmental samples is difficult and liable to variation. Future studies should both rely on molecular approaches and address the micro-heterogeneity of soil when investigating physico-chemical associations with the presence of B. pseudomallei. |
| format |
article |
| author |
L. Manivanh A. Pierret S. Rattanavong O. Kounnavongsa Y. Buisson I. Elliott J. -L. Maeght K. Xayyathip J. Silisouk M. Vongsouvath R. Phetsouvanh P. N. Newton G. Lacombe O. Ribolzi E. Rochelle-Newall D. A. B. Dance |
| author_facet |
L. Manivanh A. Pierret S. Rattanavong O. Kounnavongsa Y. Buisson I. Elliott J. -L. Maeght K. Xayyathip J. Silisouk M. Vongsouvath R. Phetsouvanh P. N. Newton G. Lacombe O. Ribolzi E. Rochelle-Newall D. A. B. Dance |
| author_sort |
L. Manivanh |
| title |
Burkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties |
| title_short |
Burkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties |
| title_full |
Burkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties |
| title_fullStr |
Burkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties |
| title_full_unstemmed |
Burkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties |
| title_sort |
burkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/689df61bb11a4ffbbc548f4d9702e963 |
| work_keys_str_mv |
AT lmanivanh burkholderiapseudomalleiinalowlandricepaddyseasonalchangesandinfluenceofsoildepthandphysicochemicalproperties AT apierret burkholderiapseudomalleiinalowlandricepaddyseasonalchangesandinfluenceofsoildepthandphysicochemicalproperties AT srattanavong burkholderiapseudomalleiinalowlandricepaddyseasonalchangesandinfluenceofsoildepthandphysicochemicalproperties AT okounnavongsa burkholderiapseudomalleiinalowlandricepaddyseasonalchangesandinfluenceofsoildepthandphysicochemicalproperties AT ybuisson burkholderiapseudomalleiinalowlandricepaddyseasonalchangesandinfluenceofsoildepthandphysicochemicalproperties AT ielliott burkholderiapseudomalleiinalowlandricepaddyseasonalchangesandinfluenceofsoildepthandphysicochemicalproperties AT jlmaeght burkholderiapseudomalleiinalowlandricepaddyseasonalchangesandinfluenceofsoildepthandphysicochemicalproperties AT kxayyathip burkholderiapseudomalleiinalowlandricepaddyseasonalchangesandinfluenceofsoildepthandphysicochemicalproperties AT jsilisouk burkholderiapseudomalleiinalowlandricepaddyseasonalchangesandinfluenceofsoildepthandphysicochemicalproperties AT mvongsouvath burkholderiapseudomalleiinalowlandricepaddyseasonalchangesandinfluenceofsoildepthandphysicochemicalproperties AT rphetsouvanh burkholderiapseudomalleiinalowlandricepaddyseasonalchangesandinfluenceofsoildepthandphysicochemicalproperties AT pnnewton burkholderiapseudomalleiinalowlandricepaddyseasonalchangesandinfluenceofsoildepthandphysicochemicalproperties AT glacombe burkholderiapseudomalleiinalowlandricepaddyseasonalchangesandinfluenceofsoildepthandphysicochemicalproperties AT oribolzi burkholderiapseudomalleiinalowlandricepaddyseasonalchangesandinfluenceofsoildepthandphysicochemicalproperties AT erochellenewall burkholderiapseudomalleiinalowlandricepaddyseasonalchangesandinfluenceofsoildepthandphysicochemicalproperties AT dabdance burkholderiapseudomalleiinalowlandricepaddyseasonalchangesandinfluenceofsoildepthandphysicochemicalproperties |
| _version_ |
1718385153496055808 |