A novel upregulated LncRNA‐AC026150.8 promotes chemo‐resistance and predicts poor prognosis in acute myeloid leukemia

Abstract Background AML is a common hematological malignancy with poor prognosis, the pathogenesis is still unclear. lncRNA takes part in occurrence and development of AML. This research aims to explore new differentially expressed lncRNAs and their effects on AML. Methods Database‐based bioinformat...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Henan Zhang, Yue Zhao, Xuan Liu, Yusi Liu, Xiaohui Wang, Yu Fu, Shuang Fu, Jihong Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	acute myeloid leukemia bioinformatics cancer biology LncRNA prognosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/689f2ca585c24685ab3641dbe9572cb0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:689f2ca585c24685ab3641dbe9572cb0
record_format	dspace
spelling	oai:doaj.org-article:689f2ca585c24685ab3641dbe9572cb02021-12-01T04:49:16ZA novel upregulated LncRNA‐AC026150.8 promotes chemo‐resistance and predicts poor prognosis in acute myeloid leukemia2045-763410.1002/cam4.4349https://doaj.org/article/689f2ca585c24685ab3641dbe9572cb02021-12-01T00:00:00Zhttps://doi.org/10.1002/cam4.4349https://doaj.org/toc/2045-7634Abstract Background AML is a common hematological malignancy with poor prognosis, the pathogenesis is still unclear. lncRNA takes part in occurrence and development of AML. This research aims to explore new differentially expressed lncRNAs and their effects on AML. Methods Database‐based bioinformatics analysis was performed to screen differentially expressed lncRNA in AML, real‐time PCR was used to analyze gene expression. Kaplan–Meier survival analysis was performed to determine prognostic effect of AC026150.8 in AML. The cell drug resistance experiment was performed to test effect of AC026150.8 on chemo‐resistance of AML cells. Catrapid online software and RNA pull‐down, mass spectrometry, western‐blot were used to predict and verify the combination of AC026150.8 and RNA splicing factors. Results AC026150.8 was upregulated in AML patients and related to poor prognosis. High leukocyte counts, FAB classification, MLL‐AF9 expression and NPM1 mutations were associated with high AC026150.8 expression. Upregulated of AC026150.8 increased the drug resistance of AML cells. AC026150.8 could be combined with splicing factor PCBP1. Conclusions For the first time, our study found that the upregulated AC026150.8 in AML is related to poor prognosis, overexpression of AC026150.8 could increase drug resistance of AML cells, and confirmed its scaffolding effect in combination with splicing factors. It is necessary to further study AC026150.8 and its downstream target genes to clarify the mechanism of AC026150.8 in AML.Henan ZhangYue ZhaoXuan LiuYusi LiuXiaohui WangYu FuShuang FuJihong ZhangWileyarticleacute myeloid leukemiabioinformaticscancer biologyLncRNAprognosisNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancer Medicine, Vol 10, Iss 23, Pp 8614-8629 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	acute myeloid leukemia bioinformatics cancer biology LncRNA prognosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	acute myeloid leukemia bioinformatics cancer biology LncRNA prognosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Henan Zhang Yue Zhao Xuan Liu Yusi Liu Xiaohui Wang Yu Fu Shuang Fu Jihong Zhang A novel upregulated LncRNA‐AC026150.8 promotes chemo‐resistance and predicts poor prognosis in acute myeloid leukemia
description	Abstract Background AML is a common hematological malignancy with poor prognosis, the pathogenesis is still unclear. lncRNA takes part in occurrence and development of AML. This research aims to explore new differentially expressed lncRNAs and their effects on AML. Methods Database‐based bioinformatics analysis was performed to screen differentially expressed lncRNA in AML, real‐time PCR was used to analyze gene expression. Kaplan–Meier survival analysis was performed to determine prognostic effect of AC026150.8 in AML. The cell drug resistance experiment was performed to test effect of AC026150.8 on chemo‐resistance of AML cells. Catrapid online software and RNA pull‐down, mass spectrometry, western‐blot were used to predict and verify the combination of AC026150.8 and RNA splicing factors. Results AC026150.8 was upregulated in AML patients and related to poor prognosis. High leukocyte counts, FAB classification, MLL‐AF9 expression and NPM1 mutations were associated with high AC026150.8 expression. Upregulated of AC026150.8 increased the drug resistance of AML cells. AC026150.8 could be combined with splicing factor PCBP1. Conclusions For the first time, our study found that the upregulated AC026150.8 in AML is related to poor prognosis, overexpression of AC026150.8 could increase drug resistance of AML cells, and confirmed its scaffolding effect in combination with splicing factors. It is necessary to further study AC026150.8 and its downstream target genes to clarify the mechanism of AC026150.8 in AML.
format	article
author	Henan Zhang Yue Zhao Xuan Liu Yusi Liu Xiaohui Wang Yu Fu Shuang Fu Jihong Zhang
author_facet	Henan Zhang Yue Zhao Xuan Liu Yusi Liu Xiaohui Wang Yu Fu Shuang Fu Jihong Zhang
author_sort	Henan Zhang
title	A novel upregulated LncRNA‐AC026150.8 promotes chemo‐resistance and predicts poor prognosis in acute myeloid leukemia
title_short	A novel upregulated LncRNA‐AC026150.8 promotes chemo‐resistance and predicts poor prognosis in acute myeloid leukemia
title_full	A novel upregulated LncRNA‐AC026150.8 promotes chemo‐resistance and predicts poor prognosis in acute myeloid leukemia
title_fullStr	A novel upregulated LncRNA‐AC026150.8 promotes chemo‐resistance and predicts poor prognosis in acute myeloid leukemia
title_full_unstemmed	A novel upregulated LncRNA‐AC026150.8 promotes chemo‐resistance and predicts poor prognosis in acute myeloid leukemia
title_sort	novel upregulated lncrna‐ac026150.8 promotes chemo‐resistance and predicts poor prognosis in acute myeloid leukemia
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/689f2ca585c24685ab3641dbe9572cb0
work_keys_str_mv	AT henanzhang anovelupregulatedlncrnaac0261508promoteschemoresistanceandpredictspoorprognosisinacutemyeloidleukemia AT yuezhao anovelupregulatedlncrnaac0261508promoteschemoresistanceandpredictspoorprognosisinacutemyeloidleukemia AT xuanliu anovelupregulatedlncrnaac0261508promoteschemoresistanceandpredictspoorprognosisinacutemyeloidleukemia AT yusiliu anovelupregulatedlncrnaac0261508promoteschemoresistanceandpredictspoorprognosisinacutemyeloidleukemia AT xiaohuiwang anovelupregulatedlncrnaac0261508promoteschemoresistanceandpredictspoorprognosisinacutemyeloidleukemia AT yufu anovelupregulatedlncrnaac0261508promoteschemoresistanceandpredictspoorprognosisinacutemyeloidleukemia AT shuangfu anovelupregulatedlncrnaac0261508promoteschemoresistanceandpredictspoorprognosisinacutemyeloidleukemia AT jihongzhang anovelupregulatedlncrnaac0261508promoteschemoresistanceandpredictspoorprognosisinacutemyeloidleukemia AT henanzhang novelupregulatedlncrnaac0261508promoteschemoresistanceandpredictspoorprognosisinacutemyeloidleukemia AT yuezhao novelupregulatedlncrnaac0261508promoteschemoresistanceandpredictspoorprognosisinacutemyeloidleukemia AT xuanliu novelupregulatedlncrnaac0261508promoteschemoresistanceandpredictspoorprognosisinacutemyeloidleukemia AT yusiliu novelupregulatedlncrnaac0261508promoteschemoresistanceandpredictspoorprognosisinacutemyeloidleukemia AT xiaohuiwang novelupregulatedlncrnaac0261508promoteschemoresistanceandpredictspoorprognosisinacutemyeloidleukemia AT yufu novelupregulatedlncrnaac0261508promoteschemoresistanceandpredictspoorprognosisinacutemyeloidleukemia AT shuangfu novelupregulatedlncrnaac0261508promoteschemoresistanceandpredictspoorprognosisinacutemyeloidleukemia AT jihongzhang novelupregulatedlncrnaac0261508promoteschemoresistanceandpredictspoorprognosisinacutemyeloidleukemia
_version_	1718405727649792000

A novel upregulated LncRNA‐AC026150.8 promotes chemo‐resistance and predicts poor prognosis in acute myeloid leukemia

Ejemplares similares