Genomic characterization of novel IncFII-type multidrug resistant plasmids p0716-KPC and p12181-KPC from Klebsiella pneumoniae

Abstract This study aimed to genetically characterize two fully-sequenced novel IncFII-type multidrug resistant (MDR) plasmids, p0716-KPC and p12181-KPC, recovered from two different clinical Klebsiella pneumoniae isolates. p0716-KPC and p12181-KPC had a very similar genomic content. The backbones o...

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Autores principales:	Jiao Feng, Zhe Yin, Qiangyuan Zhao, Yachao Zhao, Defu Zhang, Xiaoyuan Jiang, Weili Wu, Weijun Chen, Hui Wang, Yajun Song, Yigang Tong, Jinglin Wang, Yanjun Li, Dongsheng Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/68a32239ea1e4a04abab3911299c6226
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spelling	oai:doaj.org-article:68a32239ea1e4a04abab3911299c62262021-12-02T12:32:50ZGenomic characterization of novel IncFII-type multidrug resistant plasmids p0716-KPC and p12181-KPC from Klebsiella pneumoniae10.1038/s41598-017-06283-z2045-2322https://doaj.org/article/68a32239ea1e4a04abab3911299c62262017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06283-zhttps://doaj.org/toc/2045-2322Abstract This study aimed to genetically characterize two fully-sequenced novel IncFII-type multidrug resistant (MDR) plasmids, p0716-KPC and p12181-KPC, recovered from two different clinical Klebsiella pneumoniae isolates. p0716-KPC and p12181-KPC had a very similar genomic content. The backbones of p0716-KPC/p12181-KPC contained two different replicons (belonging to a novel IncFII subtype and the Rep_3 family), the IncFIIK and IncFIIY maintenance regions, and conjugal transfer gene sets from IncFIIK-type plasmids and unknown origins. p0716-KPC and p12181-KPC carried similar three accessory resistance regions, namely ΔTn6209, a MDR region, and the bla KPC-2 region. Resistance genes bla KPC-2, mph(A), strAB, aacC2, qacEΔ1, sul1, sul2, and dfrA25, which are associated with transposons, integrons, and insertion sequence-based mobile units, were located in these accessory regions. p0716-KPC carried two additional resistance genes: aphA1a and bla TEM-1. Together, our analyses showed that p0716-KPC and p12181-KPC belong to a novel IncFII subtype and display a complex chimeric nature, and that the carbapenem resistance gene bla KPC-2 coexists with a lot of additional resistance genes on these two plasmids.Jiao FengZhe YinQiangyuan ZhaoYachao ZhaoDefu ZhangXiaoyuan JiangWeili WuWeijun ChenHui WangYajun SongYigang TongJinglin WangYanjun LiDongsheng ZhouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jiao Feng Zhe Yin Qiangyuan Zhao Yachao Zhao Defu Zhang Xiaoyuan Jiang Weili Wu Weijun Chen Hui Wang Yajun Song Yigang Tong Jinglin Wang Yanjun Li Dongsheng Zhou Genomic characterization of novel IncFII-type multidrug resistant plasmids p0716-KPC and p12181-KPC from Klebsiella pneumoniae
description	Abstract This study aimed to genetically characterize two fully-sequenced novel IncFII-type multidrug resistant (MDR) plasmids, p0716-KPC and p12181-KPC, recovered from two different clinical Klebsiella pneumoniae isolates. p0716-KPC and p12181-KPC had a very similar genomic content. The backbones of p0716-KPC/p12181-KPC contained two different replicons (belonging to a novel IncFII subtype and the Rep_3 family), the IncFIIK and IncFIIY maintenance regions, and conjugal transfer gene sets from IncFIIK-type plasmids and unknown origins. p0716-KPC and p12181-KPC carried similar three accessory resistance regions, namely ΔTn6209, a MDR region, and the bla KPC-2 region. Resistance genes bla KPC-2, mph(A), strAB, aacC2, qacEΔ1, sul1, sul2, and dfrA25, which are associated with transposons, integrons, and insertion sequence-based mobile units, were located in these accessory regions. p0716-KPC carried two additional resistance genes: aphA1a and bla TEM-1. Together, our analyses showed that p0716-KPC and p12181-KPC belong to a novel IncFII subtype and display a complex chimeric nature, and that the carbapenem resistance gene bla KPC-2 coexists with a lot of additional resistance genes on these two plasmids.
format	article
author	Jiao Feng Zhe Yin Qiangyuan Zhao Yachao Zhao Defu Zhang Xiaoyuan Jiang Weili Wu Weijun Chen Hui Wang Yajun Song Yigang Tong Jinglin Wang Yanjun Li Dongsheng Zhou
author_facet	Jiao Feng Zhe Yin Qiangyuan Zhao Yachao Zhao Defu Zhang Xiaoyuan Jiang Weili Wu Weijun Chen Hui Wang Yajun Song Yigang Tong Jinglin Wang Yanjun Li Dongsheng Zhou
author_sort	Jiao Feng
title	Genomic characterization of novel IncFII-type multidrug resistant plasmids p0716-KPC and p12181-KPC from Klebsiella pneumoniae
title_short	Genomic characterization of novel IncFII-type multidrug resistant plasmids p0716-KPC and p12181-KPC from Klebsiella pneumoniae
title_full	Genomic characterization of novel IncFII-type multidrug resistant plasmids p0716-KPC and p12181-KPC from Klebsiella pneumoniae
title_fullStr	Genomic characterization of novel IncFII-type multidrug resistant plasmids p0716-KPC and p12181-KPC from Klebsiella pneumoniae
title_full_unstemmed	Genomic characterization of novel IncFII-type multidrug resistant plasmids p0716-KPC and p12181-KPC from Klebsiella pneumoniae
title_sort	genomic characterization of novel incfii-type multidrug resistant plasmids p0716-kpc and p12181-kpc from klebsiella pneumoniae
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/68a32239ea1e4a04abab3911299c6226
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Genomic characterization of novel IncFII-type multidrug resistant plasmids p0716-KPC and p12181-KPC from Klebsiella pneumoniae

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