Seasonal Prevalence and Novel Multilocus Genotypes of Giardia duodenalis in Yaks (Bos grunniens) in Qinghai Province, Western China

Background: Giardia duodenalis is an important opportunistic zoonotic intestinal protozoon, which could parasitize yaks. However, a few studies have been conducted on the seasonal infection of G. duodenalis in yaks in China. Methods: Overall, 1,027 fecal samples were collected from yaks of two ag...

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Autores principales:	Jun-ke Song, Dan Wang, Mei Ren, Fan Yang, Pin-Xue Wang, Min Zou, Guang-hui Zhao, Qing Lin
Formato:	article
Lenguaje:	EN
Publicado:	Tehran University of Medical Sciences 2021
Materias:	giardia duodenalis multilocus sequence typing (mlst) yaks prevalence Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/68a649243e304a55a878d798cd7ae855
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spelling	oai:doaj.org-article:68a649243e304a55a878d798cd7ae8552021-11-29T07:18:54ZSeasonal Prevalence and Novel Multilocus Genotypes of Giardia duodenalis in Yaks (Bos grunniens) in Qinghai Province, Western China1735-70202008-238Xhttps://doaj.org/article/68a649243e304a55a878d798cd7ae8552021-11-01T00:00:00Zhttps://ijpa.tums.ac.ir/index.php/ijpa/article/view/2364https://doaj.org/toc/1735-7020https://doaj.org/toc/2008-238XBackground: Giardia duodenalis is an important opportunistic zoonotic intestinal protozoon, which could parasitize yaks. However, a few studies have been conducted on the seasonal infection of G. duodenalis in yaks in China. Methods: Overall, 1,027 fecal samples were collected from yaks of two age groups in seven cities of Qinghai Province, China at four seasons between May 2016 and Sep 2017. The prevalence and assemblages were analyzed by nested PCR and multilocus sequence typing (MLST). Results: The overall prevalence of G. duodenalis was 2.04% (21/1027) based on triose phosphate isomease (tpi) locus. No significant differences in prevalence of the organism in yaks were found among different sampling areas. Additionally, same result was also presented in different seasons. However, there was statistically significant difference between young yaks within 6 months (8.33%, 4/48) and adult yaks over 6 months (1.73%, 17/979). The assemblage A recognized as a zoonotic assemblage (n=3) was found in yaks (>6 months) from Xining, while assemblage E (n=18) was detected from yaks in six cities. There were 5, 2 and 3 G. duodenalis subtypes detected positive at the tpi, the β-giardin (bg), and the glutamate dehydrogenase (gdh) loci, with 2, 2 and 3 novel subtypes, respectively. Three samples were successfully sequenced at all three loci, forming 1 assemblages A multilocus genotype (MLG) and 2 assemblages E MLGs, not reported. Conclusion: This study indicated a zoonotic potential of G. duodenalis in yaks from Qinghai Province and provides basic information about the epidemiology of G. duodenalis.Jun-ke SongDan WangMei RenFan YangPin-Xue WangMin ZouGuang-hui ZhaoQing LinTehran University of Medical Sciencesarticlegiardia duodenalismultilocus sequence typing (mlst)yaksprevalenceInfectious and parasitic diseasesRC109-216ENIranian Journal of Parasitology, Vol 16, Iss 4, Pp 548-554 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	giardia duodenalis multilocus sequence typing (mlst) yaks prevalence Infectious and parasitic diseases RC109-216
spellingShingle	giardia duodenalis multilocus sequence typing (mlst) yaks prevalence Infectious and parasitic diseases RC109-216 Jun-ke Song Dan Wang Mei Ren Fan Yang Pin-Xue Wang Min Zou Guang-hui Zhao Qing Lin Seasonal Prevalence and Novel Multilocus Genotypes of Giardia duodenalis in Yaks (Bos grunniens) in Qinghai Province, Western China
description	Background: Giardia duodenalis is an important opportunistic zoonotic intestinal protozoon, which could parasitize yaks. However, a few studies have been conducted on the seasonal infection of G. duodenalis in yaks in China. Methods: Overall, 1,027 fecal samples were collected from yaks of two age groups in seven cities of Qinghai Province, China at four seasons between May 2016 and Sep 2017. The prevalence and assemblages were analyzed by nested PCR and multilocus sequence typing (MLST). Results: The overall prevalence of G. duodenalis was 2.04% (21/1027) based on triose phosphate isomease (tpi) locus. No significant differences in prevalence of the organism in yaks were found among different sampling areas. Additionally, same result was also presented in different seasons. However, there was statistically significant difference between young yaks within 6 months (8.33%, 4/48) and adult yaks over 6 months (1.73%, 17/979). The assemblage A recognized as a zoonotic assemblage (n=3) was found in yaks (>6 months) from Xining, while assemblage E (n=18) was detected from yaks in six cities. There were 5, 2 and 3 G. duodenalis subtypes detected positive at the tpi, the β-giardin (bg), and the glutamate dehydrogenase (gdh) loci, with 2, 2 and 3 novel subtypes, respectively. Three samples were successfully sequenced at all three loci, forming 1 assemblages A multilocus genotype (MLG) and 2 assemblages E MLGs, not reported. Conclusion: This study indicated a zoonotic potential of G. duodenalis in yaks from Qinghai Province and provides basic information about the epidemiology of G. duodenalis.
format	article
author	Jun-ke Song Dan Wang Mei Ren Fan Yang Pin-Xue Wang Min Zou Guang-hui Zhao Qing Lin
author_facet	Jun-ke Song Dan Wang Mei Ren Fan Yang Pin-Xue Wang Min Zou Guang-hui Zhao Qing Lin
author_sort	Jun-ke Song
title	Seasonal Prevalence and Novel Multilocus Genotypes of Giardia duodenalis in Yaks (Bos grunniens) in Qinghai Province, Western China
title_short	Seasonal Prevalence and Novel Multilocus Genotypes of Giardia duodenalis in Yaks (Bos grunniens) in Qinghai Province, Western China
title_full	Seasonal Prevalence and Novel Multilocus Genotypes of Giardia duodenalis in Yaks (Bos grunniens) in Qinghai Province, Western China
title_fullStr	Seasonal Prevalence and Novel Multilocus Genotypes of Giardia duodenalis in Yaks (Bos grunniens) in Qinghai Province, Western China
title_full_unstemmed	Seasonal Prevalence and Novel Multilocus Genotypes of Giardia duodenalis in Yaks (Bos grunniens) in Qinghai Province, Western China
title_sort	seasonal prevalence and novel multilocus genotypes of giardia duodenalis in yaks (bos grunniens) in qinghai province, western china
publisher	Tehran University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/68a649243e304a55a878d798cd7ae855
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Seasonal Prevalence and Novel Multilocus Genotypes of Giardia duodenalis in Yaks (Bos grunniens) in Qinghai Province, Western China

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