Predictive Role of the p16 Immunostaining Pattern in Atypical Cervical Biopsies with Less Common High Risk HPV Genotypes

P16 immunostaining is considered a useful surrogate of transcriptionally active high-risk (hr) HPV infection. Only strong and widespread “block-like” immunoreactivity is considered specific, whereas weak/focal p16 positive immunostaining is considered not specific, and follow-up and HPV molecular de...

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Autores principales:	Daniela Cabibi, Caterina Napolitano, Antonino Giulio Giannone, Maria Carmela Micciulla, Rossana Porcasi, Roberta Lo Coco, Liana Bosco, Manlio Vinciguerra, Giuseppina Capra
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	high-risk HPV p16 Ki67 immunohistochemistry Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/68a8921501534a0da95c83d1bad166fd
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spelling	oai:doaj.org-article:68a8921501534a0da95c83d1bad166fd2021-11-25T17:20:08ZPredictive Role of the p16 Immunostaining Pattern in Atypical Cervical Biopsies with Less Common High Risk HPV Genotypes10.3390/diagnostics111119472075-4418https://doaj.org/article/68a8921501534a0da95c83d1bad166fd2021-10-01T00:00:00Zhttps://www.mdpi.com/2075-4418/11/11/1947https://doaj.org/toc/2075-4418P16 immunostaining is considered a useful surrogate of transcriptionally active high-risk (hr) HPV infection. Only strong and widespread “block-like” immunoreactivity is considered specific, whereas weak/focal p16 positive immunostaining is considered not specific, and follow-up and HPV molecular detection is not indicated. The aim of the study was to evaluate the presence of HPV DNA and Ki67 immunostaining in 40 cervical atypical biopsies (CALs) with mild and focal histological features suggestive of HPV infection—20 cases with weak/focal p16 positive immunoreactivity and 20 cases negative for p16 expression. In 16/20 weak/focal p16 positive CALs (80%), the INNO-LiPA HPV genotyping detected hrHPV genotypes (HPV 31, 51, 56, 59, 26, 53, 66, 73, and 82). Co-infection of two or more hrHPV genotypes was often evidenced. HPV16 and 18 genotypes were never detected. Ki67 immunostaining was increased in 10/20 cases (50%). In 19/20 p16 negative CALs, hrHPV infection was absent and Ki67 was not increased. These results suggest that weak/focal p16 immunostaining represents the early stage of transcriptionally active infection, strongly related to the presence of less common hrHPV genotypes, probably with a slower transforming power, but with a potential risk of progression if the infection persists. HPV DNA genotyping and follow-up could be useful in these cases to verify if they are able to evolve into overt dysplastic changes and to improve knowledge of less common hrHPV genotypes.Daniela CabibiCaterina NapolitanoAntonino Giulio GiannoneMaria Carmela MicciullaRossana PorcasiRoberta Lo CocoLiana BoscoManlio VinciguerraGiuseppina CapraMDPI AGarticlehigh-risk HPVp16Ki67immunohistochemistryMedicine (General)R5-920ENDiagnostics, Vol 11, Iss 1947, p 1947 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	high-risk HPV p16 Ki67 immunohistochemistry Medicine (General) R5-920
spellingShingle	high-risk HPV p16 Ki67 immunohistochemistry Medicine (General) R5-920 Daniela Cabibi Caterina Napolitano Antonino Giulio Giannone Maria Carmela Micciulla Rossana Porcasi Roberta Lo Coco Liana Bosco Manlio Vinciguerra Giuseppina Capra Predictive Role of the p16 Immunostaining Pattern in Atypical Cervical Biopsies with Less Common High Risk HPV Genotypes
description	P16 immunostaining is considered a useful surrogate of transcriptionally active high-risk (hr) HPV infection. Only strong and widespread “block-like” immunoreactivity is considered specific, whereas weak/focal p16 positive immunostaining is considered not specific, and follow-up and HPV molecular detection is not indicated. The aim of the study was to evaluate the presence of HPV DNA and Ki67 immunostaining in 40 cervical atypical biopsies (CALs) with mild and focal histological features suggestive of HPV infection—20 cases with weak/focal p16 positive immunoreactivity and 20 cases negative for p16 expression. In 16/20 weak/focal p16 positive CALs (80%), the INNO-LiPA HPV genotyping detected hrHPV genotypes (HPV 31, 51, 56, 59, 26, 53, 66, 73, and 82). Co-infection of two or more hrHPV genotypes was often evidenced. HPV16 and 18 genotypes were never detected. Ki67 immunostaining was increased in 10/20 cases (50%). In 19/20 p16 negative CALs, hrHPV infection was absent and Ki67 was not increased. These results suggest that weak/focal p16 immunostaining represents the early stage of transcriptionally active infection, strongly related to the presence of less common hrHPV genotypes, probably with a slower transforming power, but with a potential risk of progression if the infection persists. HPV DNA genotyping and follow-up could be useful in these cases to verify if they are able to evolve into overt dysplastic changes and to improve knowledge of less common hrHPV genotypes.
format	article
author	Daniela Cabibi Caterina Napolitano Antonino Giulio Giannone Maria Carmela Micciulla Rossana Porcasi Roberta Lo Coco Liana Bosco Manlio Vinciguerra Giuseppina Capra
author_facet	Daniela Cabibi Caterina Napolitano Antonino Giulio Giannone Maria Carmela Micciulla Rossana Porcasi Roberta Lo Coco Liana Bosco Manlio Vinciguerra Giuseppina Capra
author_sort	Daniela Cabibi
title	Predictive Role of the p16 Immunostaining Pattern in Atypical Cervical Biopsies with Less Common High Risk HPV Genotypes
title_short	Predictive Role of the p16 Immunostaining Pattern in Atypical Cervical Biopsies with Less Common High Risk HPV Genotypes
title_full	Predictive Role of the p16 Immunostaining Pattern in Atypical Cervical Biopsies with Less Common High Risk HPV Genotypes
title_fullStr	Predictive Role of the p16 Immunostaining Pattern in Atypical Cervical Biopsies with Less Common High Risk HPV Genotypes
title_full_unstemmed	Predictive Role of the p16 Immunostaining Pattern in Atypical Cervical Biopsies with Less Common High Risk HPV Genotypes
title_sort	predictive role of the p16 immunostaining pattern in atypical cervical biopsies with less common high risk hpv genotypes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/68a8921501534a0da95c83d1bad166fd
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Predictive Role of the p16 Immunostaining Pattern in Atypical Cervical Biopsies with Less Common High Risk HPV Genotypes

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