Lethal Dose of Calcium Bentonite in Wistar Rats

Background: Calcium bentonite is a type of clay produced by a devitrification of volcanic ash which is often used as a traditional medicine to absorb toxins and waste products of metabolism. The aim of this study was to determine the lethal dose of calcium bentonite in Wistar rats to explore its tox...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dwika Audiyananda, Hendro Sudjono Yuwono, Adria Adnan
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Padjadjaran 2021
Materias:	calcium bentonite ld50 toxicity Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/68a8da91b4264145bda38fc840823b6c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:68a8da91b4264145bda38fc840823b6c
record_format	dspace
spelling	oai:doaj.org-article:68a8da91b4264145bda38fc840823b6c2021-11-17T05:17:32ZLethal Dose of Calcium Bentonite in Wistar Rats2337-433010.15850/amj.v8n2.2227https://doaj.org/article/68a8da91b4264145bda38fc840823b6c2021-06-01T00:00:00Zhttp://journal.fk.unpad.ac.id/index.php/amj/article/view/2227https://doaj.org/toc/2337-4330Background: Calcium bentonite is a type of clay produced by a devitrification of volcanic ash which is often used as a traditional medicine to absorb toxins and waste products of metabolism. The aim of this study was to determine the lethal dose of calcium bentonite in Wistar rats to explore its toxicity level and safe use. Methods: Fifty male and female Wistar rats were randomly divided into five groups for each sex. The control group was given 5 cc aquadest whereas the other four groups received calcium bentonite solution of 50 mg/kgBW, 300 mg/kgBW, 2,000 mg/kgBW, and 5,000 mg/kgBW, respectively, at a single dose. The weight was observed for up to 7 days and analyzed using the unpaired t-test and Mann-Whitney test. The death rate was calculated using the probit analysis. Median lethal dose results were then classified according to Loomis Criteria. Results: No deaths occurred at the highest dose, suggesting that the median lethal dose value of calcium bentonite was >5,000 mg/kgBW. No weight loss occurred due to the administration of calcium bentonite and a significant increase in the body weight was even observed in the male rat group. Interestingly, a significant decrease was found in the female rats group when compared to the control group. Conclusions: Calcium bentonite is classified as a practically non-toxic material with a median lethal dose of over 5,000 mg/kgBW.Dwika AudiyanandaHendro Sudjono YuwonoAdria AdnanUniversitas Padjadjaranarticlecalcium bentoniteld50toxicityMedicineRMedicine (General)R5-920ENAlthea Medical Journal, Vol 8, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	calcium bentonite ld50 toxicity Medicine R Medicine (General) R5-920
spellingShingle	calcium bentonite ld50 toxicity Medicine R Medicine (General) R5-920 Dwika Audiyananda Hendro Sudjono Yuwono Adria Adnan Lethal Dose of Calcium Bentonite in Wistar Rats
description	Background: Calcium bentonite is a type of clay produced by a devitrification of volcanic ash which is often used as a traditional medicine to absorb toxins and waste products of metabolism. The aim of this study was to determine the lethal dose of calcium bentonite in Wistar rats to explore its toxicity level and safe use. Methods: Fifty male and female Wistar rats were randomly divided into five groups for each sex. The control group was given 5 cc aquadest whereas the other four groups received calcium bentonite solution of 50 mg/kgBW, 300 mg/kgBW, 2,000 mg/kgBW, and 5,000 mg/kgBW, respectively, at a single dose. The weight was observed for up to 7 days and analyzed using the unpaired t-test and Mann-Whitney test. The death rate was calculated using the probit analysis. Median lethal dose results were then classified according to Loomis Criteria. Results: No deaths occurred at the highest dose, suggesting that the median lethal dose value of calcium bentonite was >5,000 mg/kgBW. No weight loss occurred due to the administration of calcium bentonite and a significant increase in the body weight was even observed in the male rat group. Interestingly, a significant decrease was found in the female rats group when compared to the control group. Conclusions: Calcium bentonite is classified as a practically non-toxic material with a median lethal dose of over 5,000 mg/kgBW.
format	article
author	Dwika Audiyananda Hendro Sudjono Yuwono Adria Adnan
author_facet	Dwika Audiyananda Hendro Sudjono Yuwono Adria Adnan
author_sort	Dwika Audiyananda
title	Lethal Dose of Calcium Bentonite in Wistar Rats
title_short	Lethal Dose of Calcium Bentonite in Wistar Rats
title_full	Lethal Dose of Calcium Bentonite in Wistar Rats
title_fullStr	Lethal Dose of Calcium Bentonite in Wistar Rats
title_full_unstemmed	Lethal Dose of Calcium Bentonite in Wistar Rats
title_sort	lethal dose of calcium bentonite in wistar rats
publisher	Universitas Padjadjaran
publishDate	2021
url	https://doaj.org/article/68a8da91b4264145bda38fc840823b6c
work_keys_str_mv	AT dwikaaudiyananda lethaldoseofcalciumbentoniteinwistarrats AT hendrosudjonoyuwono lethaldoseofcalciumbentoniteinwistarrats AT adriaadnan lethaldoseofcalciumbentoniteinwistarrats
_version_	1718425930218602496

Lethal Dose of Calcium Bentonite in Wistar Rats

Ejemplares similares