Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment

Off-target mutations that occur at a frequency below 0.5% can be difficult to detect. Here the authors use predicted off-target amplification to increase detection sensitivity.

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Autores principales:	Seung-Hun Kang, Wi-jae Lee, Ju-Hyun An, Jong-Hee Lee, Young-Hyun Kim, Hanseop Kim, Yeounsun Oh, Young-Ho Park, Yeung Bae Jin, Bong-Hyun Jun, Junho K. Hur, Sun-Uk Kim, Seung Hwan Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/68aec577321b412993220ff58b4f8fc4
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spelling	oai:doaj.org-article:68aec577321b412993220ff58b4f8fc42021-12-02T17:01:29ZPrediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment10.1038/s41467-020-17418-82041-1723https://doaj.org/article/68aec577321b412993220ff58b4f8fc42020-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17418-8https://doaj.org/toc/2041-1723Off-target mutations that occur at a frequency below 0.5% can be difficult to detect. Here the authors use predicted off-target amplification to increase detection sensitivity.Seung-Hun KangWi-jae LeeJu-Hyun AnJong-Hee LeeYoung-Hyun KimHanseop KimYeounsun OhYoung-Ho ParkYeung Bae JinBong-Hyun JunJunho K. HurSun-Uk KimSeung Hwan LeeNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Seung-Hun Kang Wi-jae Lee Ju-Hyun An Jong-Hee Lee Young-Hyun Kim Hanseop Kim Yeounsun Oh Young-Ho Park Yeung Bae Jin Bong-Hyun Jun Junho K. Hur Sun-Uk Kim Seung Hwan Lee Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment
description	Off-target mutations that occur at a frequency below 0.5% can be difficult to detect. Here the authors use predicted off-target amplification to increase detection sensitivity.
format	article
author	Seung-Hun Kang Wi-jae Lee Ju-Hyun An Jong-Hee Lee Young-Hyun Kim Hanseop Kim Yeounsun Oh Young-Ho Park Yeung Bae Jin Bong-Hyun Jun Junho K. Hur Sun-Uk Kim Seung Hwan Lee
author_facet	Seung-Hun Kang Wi-jae Lee Ju-Hyun An Jong-Hee Lee Young-Hyun Kim Hanseop Kim Yeounsun Oh Young-Ho Park Yeung Bae Jin Bong-Hyun Jun Junho K. Hur Sun-Uk Kim Seung Hwan Lee
author_sort	Seung-Hun Kang
title	Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment
title_short	Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment
title_full	Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment
title_fullStr	Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment
title_full_unstemmed	Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment
title_sort	prediction-based highly sensitive crispr off-target validation using target-specific dna enrichment
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/68aec577321b412993220ff58b4f8fc4
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Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment

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