Single-cell growth inference of Corynebacterium glutamicum reveals asymptotically linear growth

Regulation of growth and cell size is crucial for the optimization of bacterial cellular function. So far, single bacterial cells have been found to grow predominantly exponentially, which implies the need for tight regulation to maintain cell size homeostasis. Here, we characterize the growth behav...

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Autores principales:	Joris JB Messelink, Fabian Meyer, Marc Bramkamp, Chase P Broedersz
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	cell-size homeostasis single-cell growth apical growth RodA cell wall C. glutamicum Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/68b24dc3b5154d7f925c3f2aef9f4622
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spelling	oai:doaj.org-article:68b24dc3b5154d7f925c3f2aef9f46222021-11-16T08:00:35ZSingle-cell growth inference of Corynebacterium glutamicum reveals asymptotically linear growth10.7554/eLife.701062050-084Xe70106https://doaj.org/article/68b24dc3b5154d7f925c3f2aef9f46222021-10-01T00:00:00Zhttps://elifesciences.org/articles/70106https://doaj.org/toc/2050-084XRegulation of growth and cell size is crucial for the optimization of bacterial cellular function. So far, single bacterial cells have been found to grow predominantly exponentially, which implies the need for tight regulation to maintain cell size homeostasis. Here, we characterize the growth behavior of the apically growing bacterium Corynebacterium glutamicum using a novel broadly applicable inference method for single-cell growth dynamics. Using this approach, we find that C. glutamicum exhibits asymptotically linear single-cell growth. To explain this growth mode, we model elongation as being rate-limited by the apical growth mechanism. Our model accurately reproduces the inferred cell growth dynamics and is validated with elongation measurements on a transglycosylase deficient ΔrodA mutant. Finally, with simulations we show that the distribution of cell lengths is narrower for linear than exponential growth, suggesting that this asymptotically linear growth mode can act as a substitute for tight division length and division symmetry regulation.Joris JB MesselinkFabian MeyerMarc BramkampChase P BroederszeLife Sciences Publications Ltdarticlecell-size homeostasissingle-cell growthapical growthRodAcell wallC. glutamicumMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cell-size homeostasis single-cell growth apical growth RodA cell wall C. glutamicum Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	cell-size homeostasis single-cell growth apical growth RodA cell wall C. glutamicum Medicine R Science Q Biology (General) QH301-705.5 Joris JB Messelink Fabian Meyer Marc Bramkamp Chase P Broedersz Single-cell growth inference of Corynebacterium glutamicum reveals asymptotically linear growth
description	Regulation of growth and cell size is crucial for the optimization of bacterial cellular function. So far, single bacterial cells have been found to grow predominantly exponentially, which implies the need for tight regulation to maintain cell size homeostasis. Here, we characterize the growth behavior of the apically growing bacterium Corynebacterium glutamicum using a novel broadly applicable inference method for single-cell growth dynamics. Using this approach, we find that C. glutamicum exhibits asymptotically linear single-cell growth. To explain this growth mode, we model elongation as being rate-limited by the apical growth mechanism. Our model accurately reproduces the inferred cell growth dynamics and is validated with elongation measurements on a transglycosylase deficient ΔrodA mutant. Finally, with simulations we show that the distribution of cell lengths is narrower for linear than exponential growth, suggesting that this asymptotically linear growth mode can act as a substitute for tight division length and division symmetry regulation.
format	article
author	Joris JB Messelink Fabian Meyer Marc Bramkamp Chase P Broedersz
author_facet	Joris JB Messelink Fabian Meyer Marc Bramkamp Chase P Broedersz
author_sort	Joris JB Messelink
title	Single-cell growth inference of Corynebacterium glutamicum reveals asymptotically linear growth
title_short	Single-cell growth inference of Corynebacterium glutamicum reveals asymptotically linear growth
title_full	Single-cell growth inference of Corynebacterium glutamicum reveals asymptotically linear growth
title_fullStr	Single-cell growth inference of Corynebacterium glutamicum reveals asymptotically linear growth
title_full_unstemmed	Single-cell growth inference of Corynebacterium glutamicum reveals asymptotically linear growth
title_sort	single-cell growth inference of corynebacterium glutamicum reveals asymptotically linear growth
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/68b24dc3b5154d7f925c3f2aef9f4622
work_keys_str_mv	AT jorisjbmesselink singlecellgrowthinferenceofcorynebacteriumglutamicumrevealsasymptoticallylineargrowth AT fabianmeyer singlecellgrowthinferenceofcorynebacteriumglutamicumrevealsasymptoticallylineargrowth AT marcbramkamp singlecellgrowthinferenceofcorynebacteriumglutamicumrevealsasymptoticallylineargrowth AT chasepbroedersz singlecellgrowthinferenceofcorynebacteriumglutamicumrevealsasymptoticallylineargrowth
_version_	1718426608230989824

Single-cell growth inference of Corynebacterium glutamicum reveals asymptotically linear growth

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