Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip

Infectious laryngotracheitis (ILT) is a viral disease of chickens’ respiratory system that imposes considerable financial burdens on the chicken industry. Rapid, simple, and specific detection of this virus is crucial to enable proper control measures. Polymerase chain reaction (PCR)-based molecular...

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Autores principales:	Mohamed El-Tholoth, Huiwen Bai, Michael G. Mauk, Eman Anis, Haim H. Bau
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	chicken infectious laryngotracheitis virus microfluidic device quantitative real-time PCR real-time LAMP Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/68b3ed9b92c74a0e91d2a2007a165a84
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spelling	oai:doaj.org-article:68b3ed9b92c74a0e91d2a2007a165a842021-11-25T16:18:59ZMolecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip10.3390/ani111132032076-2615https://doaj.org/article/68b3ed9b92c74a0e91d2a2007a165a842021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3203https://doaj.org/toc/2076-2615Infectious laryngotracheitis (ILT) is a viral disease of chickens’ respiratory system that imposes considerable financial burdens on the chicken industry. Rapid, simple, and specific detection of this virus is crucial to enable proper control measures. Polymerase chain reaction (PCR)-based molecular tests require relatively expensive instruments and skilled personnel, confining their application to centralized laboratories. To enable chicken farms to take timely action and contain the spread of infection, we describe a rapid, simple, semi-quantitative benchtop isothermal amplification (LAMP) assay, and a field-deployable microfluidic device for the diagnosis of ILTV infection in chickens. Our assay performance was compared and favorably agreed with quantitative PCR (qPCR). The sensitivity of our real-time LAMP test is 250 genomic copies/reaction. Clinical performance of our microfluidic device using samples from diseased chickens showed 100% specificity and 100% sensitivity in comparison with benchtop LAMP assay and the gold-standard qPCR. Our method facilitates simple, specific, and rapid molecular ILTV detection in low-resource veterinary diagnostic laboratories and can be used for field molecular diagnosis of suspected ILT cases.Mohamed El-TholothHuiwen BaiMichael G. MaukEman AnisHaim H. BauMDPI AGarticlechickeninfectious laryngotracheitis virusmicrofluidic devicequantitative real-time PCRreal-time LAMPVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3203, p 3203 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chicken infectious laryngotracheitis virus microfluidic device quantitative real-time PCR real-time LAMP Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	chicken infectious laryngotracheitis virus microfluidic device quantitative real-time PCR real-time LAMP Veterinary medicine SF600-1100 Zoology QL1-991 Mohamed El-Tholoth Huiwen Bai Michael G. Mauk Eman Anis Haim H. Bau Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip
description	Infectious laryngotracheitis (ILT) is a viral disease of chickens’ respiratory system that imposes considerable financial burdens on the chicken industry. Rapid, simple, and specific detection of this virus is crucial to enable proper control measures. Polymerase chain reaction (PCR)-based molecular tests require relatively expensive instruments and skilled personnel, confining their application to centralized laboratories. To enable chicken farms to take timely action and contain the spread of infection, we describe a rapid, simple, semi-quantitative benchtop isothermal amplification (LAMP) assay, and a field-deployable microfluidic device for the diagnosis of ILTV infection in chickens. Our assay performance was compared and favorably agreed with quantitative PCR (qPCR). The sensitivity of our real-time LAMP test is 250 genomic copies/reaction. Clinical performance of our microfluidic device using samples from diseased chickens showed 100% specificity and 100% sensitivity in comparison with benchtop LAMP assay and the gold-standard qPCR. Our method facilitates simple, specific, and rapid molecular ILTV detection in low-resource veterinary diagnostic laboratories and can be used for field molecular diagnosis of suspected ILT cases.
format	article
author	Mohamed El-Tholoth Huiwen Bai Michael G. Mauk Eman Anis Haim H. Bau
author_facet	Mohamed El-Tholoth Huiwen Bai Michael G. Mauk Eman Anis Haim H. Bau
author_sort	Mohamed El-Tholoth
title	Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip
title_short	Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip
title_full	Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip
title_fullStr	Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip
title_full_unstemmed	Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip
title_sort	molecular detection of infectious laryngotracheitis virus in chickens with a microfluidic chip
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/68b3ed9b92c74a0e91d2a2007a165a84
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Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip

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