Direct Detection Electron Energy-Loss Spectroscopy: A Method to Push the Limits of Resolution and Sensitivity

Abstract In many cases, electron counting with direct detection sensors offers improved resolution, lower noise, and higher pixel density compared to conventional, indirect detection sensors for electron microscopy applications. Direct detection technology has previously been utilized, with great su...

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Autores principales:	James L. Hart, Andrew C. Lang, Asher C. Leff, Paolo Longo, Colin Trevor, Ray D. Twesten, Mitra L. Taheri
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/68b997663aa64bffadbb628d43c9d51b
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spelling	oai:doaj.org-article:68b997663aa64bffadbb628d43c9d51b2021-12-02T12:30:43ZDirect Detection Electron Energy-Loss Spectroscopy: A Method to Push the Limits of Resolution and Sensitivity10.1038/s41598-017-07709-42045-2322https://doaj.org/article/68b997663aa64bffadbb628d43c9d51b2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07709-4https://doaj.org/toc/2045-2322Abstract In many cases, electron counting with direct detection sensors offers improved resolution, lower noise, and higher pixel density compared to conventional, indirect detection sensors for electron microscopy applications. Direct detection technology has previously been utilized, with great success, for imaging and diffraction, but potential advantages for spectroscopy remain unexplored. Here we compare the performance of a direct detection sensor operated in counting mode and an indirect detection sensor (scintillator/fiber-optic/CCD) for electron energy-loss spectroscopy. Clear improvements in measured detective quantum efficiency and combined energy resolution/energy field-of-view are offered by counting mode direct detection, showing promise for efficient spectrum imaging, low-dose mapping of beam-sensitive specimens, trace element analysis, and time-resolved spectroscopy. Despite the limited counting rate imposed by the readout electronics, we show that both core-loss and low-loss spectral acquisition are practical. These developments will benefit biologists, chemists, physicists, and materials scientists alike.James L. HartAndrew C. LangAsher C. LeffPaolo LongoColin TrevorRay D. TwestenMitra L. TaheriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q James L. Hart Andrew C. Lang Asher C. Leff Paolo Longo Colin Trevor Ray D. Twesten Mitra L. Taheri Direct Detection Electron Energy-Loss Spectroscopy: A Method to Push the Limits of Resolution and Sensitivity
description	Abstract In many cases, electron counting with direct detection sensors offers improved resolution, lower noise, and higher pixel density compared to conventional, indirect detection sensors for electron microscopy applications. Direct detection technology has previously been utilized, with great success, for imaging and diffraction, but potential advantages for spectroscopy remain unexplored. Here we compare the performance of a direct detection sensor operated in counting mode and an indirect detection sensor (scintillator/fiber-optic/CCD) for electron energy-loss spectroscopy. Clear improvements in measured detective quantum efficiency and combined energy resolution/energy field-of-view are offered by counting mode direct detection, showing promise for efficient spectrum imaging, low-dose mapping of beam-sensitive specimens, trace element analysis, and time-resolved spectroscopy. Despite the limited counting rate imposed by the readout electronics, we show that both core-loss and low-loss spectral acquisition are practical. These developments will benefit biologists, chemists, physicists, and materials scientists alike.
format	article
author	James L. Hart Andrew C. Lang Asher C. Leff Paolo Longo Colin Trevor Ray D. Twesten Mitra L. Taheri
author_facet	James L. Hart Andrew C. Lang Asher C. Leff Paolo Longo Colin Trevor Ray D. Twesten Mitra L. Taheri
author_sort	James L. Hart
title	Direct Detection Electron Energy-Loss Spectroscopy: A Method to Push the Limits of Resolution and Sensitivity
title_short	Direct Detection Electron Energy-Loss Spectroscopy: A Method to Push the Limits of Resolution and Sensitivity
title_full	Direct Detection Electron Energy-Loss Spectroscopy: A Method to Push the Limits of Resolution and Sensitivity
title_fullStr	Direct Detection Electron Energy-Loss Spectroscopy: A Method to Push the Limits of Resolution and Sensitivity
title_full_unstemmed	Direct Detection Electron Energy-Loss Spectroscopy: A Method to Push the Limits of Resolution and Sensitivity
title_sort	direct detection electron energy-loss spectroscopy: a method to push the limits of resolution and sensitivity
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/68b997663aa64bffadbb628d43c9d51b
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Direct Detection Electron Energy-Loss Spectroscopy: A Method to Push the Limits of Resolution and Sensitivity

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