Aeromonas hydrophila infection induces Toll-like receptor 2 (tlr2) and associated downstream signaling in Indian catfish, Clarias magur (Hamilton, 1822)

Motile Aeromonas septicaemia (MAS), caused by Aeromonas hydrophila, is one of the most significant bacterial disease responsible for mortality in Indian catfish, Clarias magur, a potential aquaculture species in the Indian subcontinent. In fish, innate immunity elicited by pathogen recognition recep...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chinmayee Muduli, Anutosh Paria, Ranjana Srivastava, Gaurav Rathore, Kuldeep K. Lal
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	Indian catfish Clarias magur Pathogen recognition receptors (PRRs) Toll like receptor 2 (tlr2) Pro-inflammatory cytokine Aeromonas hydrophila Gene expression Medicine R
Acceso en línea:	https://doaj.org/article/68baaa116b77400482a876530d8adecd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:68baaa116b77400482a876530d8adecd
record_format	dspace
spelling	oai:doaj.org-article:68baaa116b77400482a876530d8adecd2021-12-02T15:05:07ZAeromonas hydrophila infection induces Toll-like receptor 2 (tlr2) and associated downstream signaling in Indian catfish, Clarias magur (Hamilton, 1822)10.7717/peerj.124112167-8359https://doaj.org/article/68baaa116b77400482a876530d8adecd2021-11-01T00:00:00Zhttps://peerj.com/articles/12411.pdfhttps://peerj.com/articles/12411/https://doaj.org/toc/2167-8359Motile Aeromonas septicaemia (MAS), caused by Aeromonas hydrophila, is one of the most significant bacterial disease responsible for mortality in Indian catfish, Clarias magur, a potential aquaculture species in the Indian subcontinent. In fish, innate immunity elicited by pathogen recognition receptors (PRRs) plays an important role in providing protection against bacterial infection. Information on PRRs including Toll-like receptors (tlrs) and their response to bacterial pathogens remains unexplored in magur. Toll-like receptor 2 (tlr2), a phylogenetically conserved germ-line encoded PRR recognizes specific microbial structure and trigger MyD88-dependent signaling pathway to induce release of various cytokines responsible for innate immune response. In the present study, tlr2 gene of magur was characterized and downstream signaling was studied following challenge with A. hydrophila. The full-length cDNA of magur tlr2 (mtlr2) comprised of 3,066 bp with a single open reading frame of 2,373 bp encoding 790 amino acids having a theoretical pI value of 6.11 and molecular weight of 90 kDa. Structurally, it comprised of signal peptide (1–42aa), one leucine-rich repeat region (LRR) at N-terminal (LRR1-NT: 50–73 aa) and C-terminal (LRR-CT: 588–608 aa), twenty LRRs in between, one trans-membrane (Tm) domain (609–631aa) followed by cytoplasmic TIR domain (670–783aa). Phylogenetically, mtlr2 is closely related to pangasius and channel catfish. Highest basal expression of mtlr2, myd88 and il-1β in spleen, nf-kb in anterior kidney was observed. Lowest basal expression of mtlr2 in skin and myd88, nf-kb and il-1β in muscle was detected. Significant up-regulation of mtlr2 and downstream expression occurred at 3, 8, 24 h post infection to A. hydrophila in important immune organs such as liver, spleen, intestine and kidney. These findings highlight the vital role of tlr2 in eliciting innate immune defence against A. hydrophila infection.Chinmayee MuduliAnutosh PariaRanjana SrivastavaGaurav RathoreKuldeep K. LalPeerJ Inc.articleIndian catfish Clarias magurPathogen recognition receptors (PRRs)Toll like receptor 2 (tlr2)Pro-inflammatory cytokineAeromonas hydrophilaGene expressionMedicineRENPeerJ, Vol 9, p e12411 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Indian catfish Clarias magur Pathogen recognition receptors (PRRs) Toll like receptor 2 (tlr2) Pro-inflammatory cytokine Aeromonas hydrophila Gene expression Medicine R
spellingShingle	Indian catfish Clarias magur Pathogen recognition receptors (PRRs) Toll like receptor 2 (tlr2) Pro-inflammatory cytokine Aeromonas hydrophila Gene expression Medicine R Chinmayee Muduli Anutosh Paria Ranjana Srivastava Gaurav Rathore Kuldeep K. Lal Aeromonas hydrophila infection induces Toll-like receptor 2 (tlr2) and associated downstream signaling in Indian catfish, Clarias magur (Hamilton, 1822)
description	Motile Aeromonas septicaemia (MAS), caused by Aeromonas hydrophila, is one of the most significant bacterial disease responsible for mortality in Indian catfish, Clarias magur, a potential aquaculture species in the Indian subcontinent. In fish, innate immunity elicited by pathogen recognition receptors (PRRs) plays an important role in providing protection against bacterial infection. Information on PRRs including Toll-like receptors (tlrs) and their response to bacterial pathogens remains unexplored in magur. Toll-like receptor 2 (tlr2), a phylogenetically conserved germ-line encoded PRR recognizes specific microbial structure and trigger MyD88-dependent signaling pathway to induce release of various cytokines responsible for innate immune response. In the present study, tlr2 gene of magur was characterized and downstream signaling was studied following challenge with A. hydrophila. The full-length cDNA of magur tlr2 (mtlr2) comprised of 3,066 bp with a single open reading frame of 2,373 bp encoding 790 amino acids having a theoretical pI value of 6.11 and molecular weight of 90 kDa. Structurally, it comprised of signal peptide (1–42aa), one leucine-rich repeat region (LRR) at N-terminal (LRR1-NT: 50–73 aa) and C-terminal (LRR-CT: 588–608 aa), twenty LRRs in between, one trans-membrane (Tm) domain (609–631aa) followed by cytoplasmic TIR domain (670–783aa). Phylogenetically, mtlr2 is closely related to pangasius and channel catfish. Highest basal expression of mtlr2, myd88 and il-1β in spleen, nf-kb in anterior kidney was observed. Lowest basal expression of mtlr2 in skin and myd88, nf-kb and il-1β in muscle was detected. Significant up-regulation of mtlr2 and downstream expression occurred at 3, 8, 24 h post infection to A. hydrophila in important immune organs such as liver, spleen, intestine and kidney. These findings highlight the vital role of tlr2 in eliciting innate immune defence against A. hydrophila infection.
format	article
author	Chinmayee Muduli Anutosh Paria Ranjana Srivastava Gaurav Rathore Kuldeep K. Lal
author_facet	Chinmayee Muduli Anutosh Paria Ranjana Srivastava Gaurav Rathore Kuldeep K. Lal
author_sort	Chinmayee Muduli
title	Aeromonas hydrophila infection induces Toll-like receptor 2 (tlr2) and associated downstream signaling in Indian catfish, Clarias magur (Hamilton, 1822)
title_short	Aeromonas hydrophila infection induces Toll-like receptor 2 (tlr2) and associated downstream signaling in Indian catfish, Clarias magur (Hamilton, 1822)
title_full	Aeromonas hydrophila infection induces Toll-like receptor 2 (tlr2) and associated downstream signaling in Indian catfish, Clarias magur (Hamilton, 1822)
title_fullStr	Aeromonas hydrophila infection induces Toll-like receptor 2 (tlr2) and associated downstream signaling in Indian catfish, Clarias magur (Hamilton, 1822)
title_full_unstemmed	Aeromonas hydrophila infection induces Toll-like receptor 2 (tlr2) and associated downstream signaling in Indian catfish, Clarias magur (Hamilton, 1822)
title_sort	aeromonas hydrophila infection induces toll-like receptor 2 (tlr2) and associated downstream signaling in indian catfish, clarias magur (hamilton, 1822)
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/68baaa116b77400482a876530d8adecd
work_keys_str_mv	AT chinmayeemuduli aeromonashydrophilainfectioninducestolllikereceptor2tlr2andassociateddownstreamsignalinginindiancatfishclariasmagurhamilton1822 AT anutoshparia aeromonashydrophilainfectioninducestolllikereceptor2tlr2andassociateddownstreamsignalinginindiancatfishclariasmagurhamilton1822 AT ranjanasrivastava aeromonashydrophilainfectioninducestolllikereceptor2tlr2andassociateddownstreamsignalinginindiancatfishclariasmagurhamilton1822 AT gauravrathore aeromonashydrophilainfectioninducestolllikereceptor2tlr2andassociateddownstreamsignalinginindiancatfishclariasmagurhamilton1822 AT kuldeepklal aeromonashydrophilainfectioninducestolllikereceptor2tlr2andassociateddownstreamsignalinginindiancatfishclariasmagurhamilton1822
_version_	1718388959641337856

Aeromonas hydrophila infection induces Toll-like receptor 2 (tlr2) and associated downstream signaling in Indian catfish, Clarias magur (Hamilton, 1822)

Ejemplares similares