Silk fibroin/cartilage extracellular matrix scaffolds with sequential delivery of TGF-β3 for chondrogenic differentiation of adipose-derived stem cells

Silk fibroin/cartilage extracellular matrix scaffolds with sequential delivery of TGF-β3 for chondrogenic differentiation of adipose-derived stem cells

Qiang Yang,1,* Bin-Hong Teng,2,* Li-Na Wang,3 Kun Li,2 Chen Xu,2 Xin-Long Ma,1 Yang Zhang,1 De-Ling Kong,3 Lian-Yong Wang,3 Yan-Hong Zhao2 1Department of Spine Surgery, Tianjin Hospital, Tianjin , People’s Republic of China; 2School and Hospital of Stomatology, Tianjin Medical University,...

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Autores principales: Yang Q, Teng B, Wang L, Li K, Xu C, Ma X, Zhang Y, Kong D, Zhao Y
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2017
Materias:
cartilage tissue engineering
composite scaffold
silk fibroin
decellularized cartilage extracellular matrix
adipose-derived stem cells
transforming growth factor β3 (TGF-β3)
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/68bb1ba6d8574ab5a909716dc6c6fa0f
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spelling oai:doaj.org-article:68bb1ba6d8574ab5a909716dc6c6fa0f2021-12-02T00:44:32ZSilk fibroin/cartilage extracellular matrix scaffolds with sequential delivery of TGF-β3 for chondrogenic differentiation of adipose-derived stem cells1178-2013https://doaj.org/article/68bb1ba6d8574ab5a909716dc6c6fa0f2017-09-01T00:00:00Zhttps://www.dovepress.com/silk-fibroincartilage-extracellular-matrix-scaffolds-with-sequential-d-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Qiang Yang,1,* Bin-Hong Teng,2,* Li-Na Wang,3 Kun Li,2 Chen Xu,2 Xin-Long Ma,1 Yang Zhang,1 De-Ling Kong,3 Lian-Yong Wang,3 Yan-Hong Zhao2 1Department of Spine Surgery, Tianjin Hospital, Tianjin , People’s Republic of China; 2School and Hospital of Stomatology, Tianjin Medical University,Tianjin, People’s Republic of China; 3The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, People’s Republic of China *These authors contributed equally to this work Abstract: A 3-D scaffold that simulates the microenvironment in vivo for regenerating cartilage is ideal. In this study, we combined silk fibroin and decellularized cartilage extracellular matrix by temperature gradient-guided thermal-induced phase separation to produce composite scaffolds (S/D). Resulting scaffolds had remarkable mechanical properties and biomimeticstructure, for a suitable substrate for attachment and proliferation of adipose-derived stem cells (ADSCs). Moreover, transforming growth factor β3 (TGF-β3) loaded on scaffolds showed a controlled release profile and enhanced the chondrogenic differentiation of ADSCs during the 28-day culture. The S/D scaffold itself can provide a sustained release system without the introduction of other controlled release media, which has potential for commercial and clinical applications. The results of toluidine blue, Safranin O, and immunohistochemical staining and analysis of collagen II expression showed maintenance of a chondrogenic phenotype in all scaffolds after 28-day culture. The most obvious phenomenon was with the addition of TGF-β3. S/D composite scaffolds with sequential delivery of TGF-β3 may mimic the regenerative microenvironment to enhance the chondrogenic differentiation of ADSCs in vitro. Keywords: cartilage tissue engineering, composite scaffold, silk fibroin, decellularized cartilage extracellular matrix, adipose-derived stem cells, transforming growth factor β3 (TGF-β3)Yang QTeng BWang LLi KXu CMa XZhang YKong DWang LZhao YDove Medical Pressarticlecartilage tissue engineeringcomposite scaffoldsilk fibroindecellularized cartilage extracellular matrixadipose-derived stem cellstransforming growth factor β3 (TGF-β3)Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 6721-6733 (2017)
institution DOAJ
collection DOAJ
language EN
topic cartilage tissue engineering
composite scaffold
silk fibroin
decellularized cartilage extracellular matrix
adipose-derived stem cells
transforming growth factor β3 (TGF-β3)
Medicine (General)
R5-920
spellingShingle cartilage tissue engineering
composite scaffold
silk fibroin
decellularized cartilage extracellular matrix
adipose-derived stem cells
transforming growth factor β3 (TGF-β3)
Medicine (General)
R5-920
Yang Q
Teng B
Wang L
Li K
Xu C
Ma X
Zhang Y
Kong D
Wang L
Zhao Y
Silk fibroin/cartilage extracellular matrix scaffolds with sequential delivery of TGF-β3 for chondrogenic differentiation of adipose-derived stem cells
description Qiang Yang,1,* Bin-Hong Teng,2,* Li-Na Wang,3 Kun Li,2 Chen Xu,2 Xin-Long Ma,1 Yang Zhang,1 De-Ling Kong,3 Lian-Yong Wang,3 Yan-Hong Zhao2 1Department of Spine Surgery, Tianjin Hospital, Tianjin , People’s Republic of China; 2School and Hospital of Stomatology, Tianjin Medical University,Tianjin, People’s Republic of China; 3The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, People’s Republic of China *These authors contributed equally to this work Abstract: A 3-D scaffold that simulates the microenvironment in vivo for regenerating cartilage is ideal. In this study, we combined silk fibroin and decellularized cartilage extracellular matrix by temperature gradient-guided thermal-induced phase separation to produce composite scaffolds (S/D). Resulting scaffolds had remarkable mechanical properties and biomimeticstructure, for a suitable substrate for attachment and proliferation of adipose-derived stem cells (ADSCs). Moreover, transforming growth factor β3 (TGF-β3) loaded on scaffolds showed a controlled release profile and enhanced the chondrogenic differentiation of ADSCs during the 28-day culture. The S/D scaffold itself can provide a sustained release system without the introduction of other controlled release media, which has potential for commercial and clinical applications. The results of toluidine blue, Safranin O, and immunohistochemical staining and analysis of collagen II expression showed maintenance of a chondrogenic phenotype in all scaffolds after 28-day culture. The most obvious phenomenon was with the addition of TGF-β3. S/D composite scaffolds with sequential delivery of TGF-β3 may mimic the regenerative microenvironment to enhance the chondrogenic differentiation of ADSCs in vitro. Keywords: cartilage tissue engineering, composite scaffold, silk fibroin, decellularized cartilage extracellular matrix, adipose-derived stem cells, transforming growth factor β3 (TGF-β3)
format article
author Yang Q
Teng B
Wang L
Li K
Xu C
Ma X
Zhang Y
Kong D
Wang L
Zhao Y
author_facet Yang Q
Teng B
Wang L
Li K
Xu C
Ma X
Zhang Y
Kong D
Wang L
Zhao Y
author_sort Yang Q
title Silk fibroin/cartilage extracellular matrix scaffolds with sequential delivery of TGF-β3 for chondrogenic differentiation of adipose-derived stem cells
title_short Silk fibroin/cartilage extracellular matrix scaffolds with sequential delivery of TGF-β3 for chondrogenic differentiation of adipose-derived stem cells
title_full Silk fibroin/cartilage extracellular matrix scaffolds with sequential delivery of TGF-β3 for chondrogenic differentiation of adipose-derived stem cells
title_fullStr Silk fibroin/cartilage extracellular matrix scaffolds with sequential delivery of TGF-β3 for chondrogenic differentiation of adipose-derived stem cells
title_full_unstemmed Silk fibroin/cartilage extracellular matrix scaffolds with sequential delivery of TGF-β3 for chondrogenic differentiation of adipose-derived stem cells
title_sort silk fibroin/cartilage extracellular matrix scaffolds with sequential delivery of tgf-β3 for chondrogenic differentiation of adipose-derived stem cells
publisher Dove Medical Press
publishDate 2017
url https://doaj.org/article/68bb1ba6d8574ab5a909716dc6c6fa0f
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