Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination.

Dengue virus (DENV) is a flavivirus that causes marked human morbidity and mortality worldwide, and is transmitted to humans by Aedes aegypti mosquitoes. Habitat expansion of Aedes, mainly due to climate change and increasing overlap between urban and wild habitats, places nearly half of the world&#...

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Autores principales:	Alejandro Marin-Lopez, Junjun Jiang, Yuchen Wang, Yongguo Cao, Tyler MacNeil, Andrew K Hastings, Erol Fikrig
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/68c80c23a5744764895c92cda4742352
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spelling	oai:doaj.org-article:68c80c23a5744764895c92cda47423522021-11-25T06:31:46ZAedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination.1935-27271935-273510.1371/journal.pntd.0009442https://doaj.org/article/68c80c23a5744764895c92cda47423522021-06-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009442https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735Dengue virus (DENV) is a flavivirus that causes marked human morbidity and mortality worldwide, and is transmitted to humans by Aedes aegypti mosquitoes. Habitat expansion of Aedes, mainly due to climate change and increasing overlap between urban and wild habitats, places nearly half of the world's population at risk for DENV infection. After a bloodmeal from a DENV-infected host, the virus enters the mosquito midgut. Next, the virus migrates to, and replicates in, other tissues, like salivary glands. Successful viral transmission occurs when the infected mosquito takes another blood meal on a susceptible host and DENV is released from the salivary gland via saliva into the skin. During viral dissemination in the mosquito and transmission to a new mammalian host, DENV interacts with a variety of vector proteins, which are uniquely important during each phase of the viral cycle. Our study focuses on the interaction between DENV particles and protein components in the A. aegypti vector. We performed a mass spectrometry assay where we identified a set of A. aegypti salivary gland proteins which potentially interact with the DENV virion. Using dsRNA to silence gene expression, we analyzed the role of these proteins in viral infectivity. Two of these candidates, a synaptosomal-associated protein (AeSNAP) and a calcium transporter ATPase (ATPase) appear to play a role in viral replication both in vitro and in vivo, observing a ubiquitous expression of these proteins in the mosquito. These findings suggest that AeSNAP plays a protective role during DENV infection of mosquitoes and that ATPase protein is required for DENV during amplification within the vector.Alejandro Marin-LopezJunjun JiangYuchen WangYongguo CaoTyler MacNeilAndrew K HastingsErol FikrigPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0009442 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Alejandro Marin-Lopez Junjun Jiang Yuchen Wang Yongguo Cao Tyler MacNeil Andrew K Hastings Erol Fikrig Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination.
description	Dengue virus (DENV) is a flavivirus that causes marked human morbidity and mortality worldwide, and is transmitted to humans by Aedes aegypti mosquitoes. Habitat expansion of Aedes, mainly due to climate change and increasing overlap between urban and wild habitats, places nearly half of the world's population at risk for DENV infection. After a bloodmeal from a DENV-infected host, the virus enters the mosquito midgut. Next, the virus migrates to, and replicates in, other tissues, like salivary glands. Successful viral transmission occurs when the infected mosquito takes another blood meal on a susceptible host and DENV is released from the salivary gland via saliva into the skin. During viral dissemination in the mosquito and transmission to a new mammalian host, DENV interacts with a variety of vector proteins, which are uniquely important during each phase of the viral cycle. Our study focuses on the interaction between DENV particles and protein components in the A. aegypti vector. We performed a mass spectrometry assay where we identified a set of A. aegypti salivary gland proteins which potentially interact with the DENV virion. Using dsRNA to silence gene expression, we analyzed the role of these proteins in viral infectivity. Two of these candidates, a synaptosomal-associated protein (AeSNAP) and a calcium transporter ATPase (ATPase) appear to play a role in viral replication both in vitro and in vivo, observing a ubiquitous expression of these proteins in the mosquito. These findings suggest that AeSNAP plays a protective role during DENV infection of mosquitoes and that ATPase protein is required for DENV during amplification within the vector.
format	article
author	Alejandro Marin-Lopez Junjun Jiang Yuchen Wang Yongguo Cao Tyler MacNeil Andrew K Hastings Erol Fikrig
author_facet	Alejandro Marin-Lopez Junjun Jiang Yuchen Wang Yongguo Cao Tyler MacNeil Andrew K Hastings Erol Fikrig
author_sort	Alejandro Marin-Lopez
title	Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination.
title_short	Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination.
title_full	Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination.
title_fullStr	Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination.
title_full_unstemmed	Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination.
title_sort	aedes aegypti snap and a calcium transporter atpase influence dengue virus dissemination.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/68c80c23a5744764895c92cda4742352
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Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination.

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