A Microfluidic Chip for Detecting Cholangiocarcinoma Cells in Human Bile

Abstract Cholangiocarcinoma (CCA), a biliary tract malignancy, accounts for 20% of all liver cancers. There are several existing methods for diagnosis of CCA, though they are generally expensive, laborious, and suffer from low detection rates. Herein we first developed a means of partially purifying...

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Autores principales:	Lien-Yu Hung, Nai-Jung Chiang, Wei-Chun Tsai, Chien-Yu Fu, Yu-Chun Wang, Yan-Shen Shan, Gwo-Bin Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/68caaa3c211e45748959dc8267095cdd
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spelling	oai:doaj.org-article:68caaa3c211e45748959dc8267095cdd2021-12-02T11:52:44ZA Microfluidic Chip for Detecting Cholangiocarcinoma Cells in Human Bile10.1038/s41598-017-04056-22045-2322https://doaj.org/article/68caaa3c211e45748959dc8267095cdd2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04056-2https://doaj.org/toc/2045-2322Abstract Cholangiocarcinoma (CCA), a biliary tract malignancy, accounts for 20% of all liver cancers. There are several existing methods for diagnosis of CCA, though they are generally expensive, laborious, and suffer from low detection rates. Herein we first developed a means of partially purifying human bile for consequent injection into a microfluidic chip. Then, the novel microfluidic system, which featured 1) a cell capture module, 2) an immunofluorescence (IF) staining module featuring two CCA-specific biomarkers, and 3) an optical detection module for visualization of antibody probes bound to these CCA marker proteins, was used to detect bile duct cancer cells within partially purified bile samples. As a proof of concept, CCA cells were successfully captured and identified from CCA cell cultures, blood samples inoculated with CCA cells, and clinical bile specimens. In 7.5 ml of bile, this system could detect >2, 0, and 1 positive cells in advanced stage patients, healthy patients, and chemotherapy-treated patients, respectively. In conclusion, our microfluidic system could be a promising tool for detection of cancer cells in bile, even at the earliest stages of CCA when cancer cells are at low densities relative to the total population of epithelial cells.Lien-Yu HungNai-Jung ChiangWei-Chun TsaiChien-Yu FuYu-Chun WangYan-Shen ShanGwo-Bin LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lien-Yu Hung Nai-Jung Chiang Wei-Chun Tsai Chien-Yu Fu Yu-Chun Wang Yan-Shen Shan Gwo-Bin Lee A Microfluidic Chip for Detecting Cholangiocarcinoma Cells in Human Bile
description	Abstract Cholangiocarcinoma (CCA), a biliary tract malignancy, accounts for 20% of all liver cancers. There are several existing methods for diagnosis of CCA, though they are generally expensive, laborious, and suffer from low detection rates. Herein we first developed a means of partially purifying human bile for consequent injection into a microfluidic chip. Then, the novel microfluidic system, which featured 1) a cell capture module, 2) an immunofluorescence (IF) staining module featuring two CCA-specific biomarkers, and 3) an optical detection module for visualization of antibody probes bound to these CCA marker proteins, was used to detect bile duct cancer cells within partially purified bile samples. As a proof of concept, CCA cells were successfully captured and identified from CCA cell cultures, blood samples inoculated with CCA cells, and clinical bile specimens. In 7.5 ml of bile, this system could detect >2, 0, and 1 positive cells in advanced stage patients, healthy patients, and chemotherapy-treated patients, respectively. In conclusion, our microfluidic system could be a promising tool for detection of cancer cells in bile, even at the earliest stages of CCA when cancer cells are at low densities relative to the total population of epithelial cells.
format	article
author	Lien-Yu Hung Nai-Jung Chiang Wei-Chun Tsai Chien-Yu Fu Yu-Chun Wang Yan-Shen Shan Gwo-Bin Lee
author_facet	Lien-Yu Hung Nai-Jung Chiang Wei-Chun Tsai Chien-Yu Fu Yu-Chun Wang Yan-Shen Shan Gwo-Bin Lee
author_sort	Lien-Yu Hung
title	A Microfluidic Chip for Detecting Cholangiocarcinoma Cells in Human Bile
title_short	A Microfluidic Chip for Detecting Cholangiocarcinoma Cells in Human Bile
title_full	A Microfluidic Chip for Detecting Cholangiocarcinoma Cells in Human Bile
title_fullStr	A Microfluidic Chip for Detecting Cholangiocarcinoma Cells in Human Bile
title_full_unstemmed	A Microfluidic Chip for Detecting Cholangiocarcinoma Cells in Human Bile
title_sort	microfluidic chip for detecting cholangiocarcinoma cells in human bile
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/68caaa3c211e45748959dc8267095cdd
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A Microfluidic Chip for Detecting Cholangiocarcinoma Cells in Human Bile

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