Gold nanoparticles allow detection of early-stage edema in mice via computed tomography imaging

Jenny Domey, Ulf Teichgräber, Ingrid Hilger Institute of Diagnostic and Interventional Radiology, University Hospital Jena, Jena, Germany Abstract: Due to their high X-ray attenuation, gold nanoparticles (GNPs) emerged as preclinical contrast agents by giving high vasculature contrast. Fo...

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Autores principales:	Domey J, Teichgräber U, Hilger I
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/68cb93d4e8e04363ad3f27cb0e1b0085
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spelling	oai:doaj.org-article:68cb93d4e8e04363ad3f27cb0e1b00852021-12-02T00:44:33ZGold nanoparticles allow detection of early-stage edema in mice via computed tomography imaging1178-2013https://doaj.org/article/68cb93d4e8e04363ad3f27cb0e1b00852015-06-01T00:00:00Zhttp://www.dovepress.com/gold-nanoparticles-allow-detection-of-early-stage-edema-in-mice-via-co-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jenny Domey, Ulf Teichgräber, Ingrid Hilger Institute of Diagnostic and Interventional Radiology, University Hospital Jena, Jena, Germany Abstract: Due to their high X-ray attenuation, gold nanoparticles (GNPs) emerged as preclinical contrast agents by giving high vasculature contrast. For this reason, GNPs are regularly applied for computed tomography (CT) imaging of tumors but not for the visualization of inflammation. The aim of this study was to evaluate the biocompatibility and applicability of preclinical GNPs (AuroVist™) of two different sizes (1.9 nm and 15 nm) for the detection of inflammation-associated phagocytes in early-stage edema. Both GNP variants were stable under in vitro conditions and achieved high micro-CT (mCT) contrast after embedment into agarose. Fifteen-nanometer GNPs were detected after uptake into macrophages via mCT imaging exhibiting higher X-ray contrast than cells treated with 1.9 nm GNPs and untreated ones. Both 1.9 nm and 15 nm GNPs exhibited good biocompatibility on murine macrophages according to ATP and cellular dehydrogenase levels. Reactive oxygen species levels produced by phagocytic cells decreased significantly (P≤0.05) after co-incubation with GNPs regardless of the size of the nanoparticle (NP) in comparison to untreated control cells. In vivo mCT studies of inflammation imaging revealed that GNPs with a diameter of 15 nm accumulated within subcutaneous edema 2 hours after injection with a maximum signaling 8 hours postinjection and could be detected up to 48 hours within the edema region. In contrast, 1.9 nm GNPs were not shown to accumulate at the site of the inflammation region and were mostly excreted via the renal system 2–4 hours after injection. In conclusion, our study demonstrated that both GNP variants (1.9 nm and 15 nm) were stable and biocompatible under in vitro conditions. However, only 15 nm NPs have the potential as contrast agent for phagocyte labeling and applications in CT imaging of inflammation on a cellular level. Keywords: inflammation, GNP, phagocytes, AuroVist™, biocompatibilityDomey JTeichgräber UHilger IDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 3803-3814 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Domey J Teichgräber U Hilger I Gold nanoparticles allow detection of early-stage edema in mice via computed tomography imaging
description	Jenny Domey, Ulf Teichgräber, Ingrid Hilger Institute of Diagnostic and Interventional Radiology, University Hospital Jena, Jena, Germany Abstract: Due to their high X-ray attenuation, gold nanoparticles (GNPs) emerged as preclinical contrast agents by giving high vasculature contrast. For this reason, GNPs are regularly applied for computed tomography (CT) imaging of tumors but not for the visualization of inflammation. The aim of this study was to evaluate the biocompatibility and applicability of preclinical GNPs (AuroVist™) of two different sizes (1.9 nm and 15 nm) for the detection of inflammation-associated phagocytes in early-stage edema. Both GNP variants were stable under in vitro conditions and achieved high micro-CT (mCT) contrast after embedment into agarose. Fifteen-nanometer GNPs were detected after uptake into macrophages via mCT imaging exhibiting higher X-ray contrast than cells treated with 1.9 nm GNPs and untreated ones. Both 1.9 nm and 15 nm GNPs exhibited good biocompatibility on murine macrophages according to ATP and cellular dehydrogenase levels. Reactive oxygen species levels produced by phagocytic cells decreased significantly (P≤0.05) after co-incubation with GNPs regardless of the size of the nanoparticle (NP) in comparison to untreated control cells. In vivo mCT studies of inflammation imaging revealed that GNPs with a diameter of 15 nm accumulated within subcutaneous edema 2 hours after injection with a maximum signaling 8 hours postinjection and could be detected up to 48 hours within the edema region. In contrast, 1.9 nm GNPs were not shown to accumulate at the site of the inflammation region and were mostly excreted via the renal system 2–4 hours after injection. In conclusion, our study demonstrated that both GNP variants (1.9 nm and 15 nm) were stable and biocompatible under in vitro conditions. However, only 15 nm NPs have the potential as contrast agent for phagocyte labeling and applications in CT imaging of inflammation on a cellular level. Keywords: inflammation, GNP, phagocytes, AuroVist™, biocompatibility
format	article
author	Domey J Teichgräber U Hilger I
author_facet	Domey J Teichgräber U Hilger I
author_sort	Domey J
title	Gold nanoparticles allow detection of early-stage edema in mice via computed tomography imaging
title_short	Gold nanoparticles allow detection of early-stage edema in mice via computed tomography imaging
title_full	Gold nanoparticles allow detection of early-stage edema in mice via computed tomography imaging
title_fullStr	Gold nanoparticles allow detection of early-stage edema in mice via computed tomography imaging
title_full_unstemmed	Gold nanoparticles allow detection of early-stage edema in mice via computed tomography imaging
title_sort	gold nanoparticles allow detection of early-stage edema in mice via computed tomography imaging
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/68cb93d4e8e04363ad3f27cb0e1b0085
work_keys_str_mv	AT domeyj goldnanoparticlesallowdetectionofearlystageedemainmiceviacomputedtomographyimaging AT teichgraberu goldnanoparticlesallowdetectionofearlystageedemainmiceviacomputedtomographyimaging AT hilgeri goldnanoparticlesallowdetectionofearlystageedemainmiceviacomputedtomographyimaging
_version_	1718403501130776576

Gold nanoparticles allow detection of early-stage edema in mice via computed tomography imaging

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