Carotid smooth muscle contractility changes after severe burn

Abstract Severe burns result in cardiovascular dysfunction, but responses in the peripheral vasculature are unclear. We hypothesize that severe burns disturb arterial contractility through acute changes in adrenergic and cholinergic receptor function. To address this, we investigated the changes in...

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Autores principales:	Kevin DeSpain, Charles R. Rosenfeld, Ryan Huebinger, Xiaofu Wang, Jayson W. Jay, Ravi S. Radhakrishnan, Steven E. Wolf, Juquan Song
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/68cdfd1fdca14e978a1fbb0f617ea222
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spelling	oai:doaj.org-article:68cdfd1fdca14e978a1fbb0f617ea2222021-12-02T17:41:18ZCarotid smooth muscle contractility changes after severe burn10.1038/s41598-021-97732-32045-2322https://doaj.org/article/68cdfd1fdca14e978a1fbb0f617ea2222021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97732-3https://doaj.org/toc/2045-2322Abstract Severe burns result in cardiovascular dysfunction, but responses in the peripheral vasculature are unclear. We hypothesize that severe burns disturb arterial contractility through acute changes in adrenergic and cholinergic receptor function. To address this, we investigated the changes in carotid artery contractility and relaxation following a severe burn. Thirty-four adult Sprague–Dawley male rats received a 40% total body surface area (TBSA) scald burn and fluid resuscitation using the Parkland formula. Control animals received sham burn procedure. Animals were serially euthanized between 6 h and 14 days after burn and endothelium-intact common carotid arteries were used for ex vivo force/relaxation measurements. At 6 h after burn, carotid arteries from burned animals demonstrated a > 50% decrease in cumulative dose-responses to norepinephrine (p < 0.05) and to 10−7 M angiotensin II (p < 0.05). Notably, pre-constricted carotid arteries also demonstrated reduced relaxation responses to acetylcholine (p < 0.05) 6 h after burn, but not to sodium nitroprusside. Histologic examination of cross-sectional planes revealed significant increases in carotid artery wall thickness in burned rats at 6 h versus 3 days, with increased collagen expression in tunica media at 3 days (p < 0.05). Carotid artery dysfunction occurs within 6 h after severe burn, demonstrating decreased sensitivity to adrenergic- and angiotensin II-induced vasoconstriction and acetylcholine-induced relaxation.Kevin DeSpainCharles R. RosenfeldRyan HuebingerXiaofu WangJayson W. JayRavi S. RadhakrishnanSteven E. WolfJuquan SongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kevin DeSpain Charles R. Rosenfeld Ryan Huebinger Xiaofu Wang Jayson W. Jay Ravi S. Radhakrishnan Steven E. Wolf Juquan Song Carotid smooth muscle contractility changes after severe burn
description	Abstract Severe burns result in cardiovascular dysfunction, but responses in the peripheral vasculature are unclear. We hypothesize that severe burns disturb arterial contractility through acute changes in adrenergic and cholinergic receptor function. To address this, we investigated the changes in carotid artery contractility and relaxation following a severe burn. Thirty-four adult Sprague–Dawley male rats received a 40% total body surface area (TBSA) scald burn and fluid resuscitation using the Parkland formula. Control animals received sham burn procedure. Animals were serially euthanized between 6 h and 14 days after burn and endothelium-intact common carotid arteries were used for ex vivo force/relaxation measurements. At 6 h after burn, carotid arteries from burned animals demonstrated a > 50% decrease in cumulative dose-responses to norepinephrine (p < 0.05) and to 10−7 M angiotensin II (p < 0.05). Notably, pre-constricted carotid arteries also demonstrated reduced relaxation responses to acetylcholine (p < 0.05) 6 h after burn, but not to sodium nitroprusside. Histologic examination of cross-sectional planes revealed significant increases in carotid artery wall thickness in burned rats at 6 h versus 3 days, with increased collagen expression in tunica media at 3 days (p < 0.05). Carotid artery dysfunction occurs within 6 h after severe burn, demonstrating decreased sensitivity to adrenergic- and angiotensin II-induced vasoconstriction and acetylcholine-induced relaxation.
format	article
author	Kevin DeSpain Charles R. Rosenfeld Ryan Huebinger Xiaofu Wang Jayson W. Jay Ravi S. Radhakrishnan Steven E. Wolf Juquan Song
author_facet	Kevin DeSpain Charles R. Rosenfeld Ryan Huebinger Xiaofu Wang Jayson W. Jay Ravi S. Radhakrishnan Steven E. Wolf Juquan Song
author_sort	Kevin DeSpain
title	Carotid smooth muscle contractility changes after severe burn
title_short	Carotid smooth muscle contractility changes after severe burn
title_full	Carotid smooth muscle contractility changes after severe burn
title_fullStr	Carotid smooth muscle contractility changes after severe burn
title_full_unstemmed	Carotid smooth muscle contractility changes after severe burn
title_sort	carotid smooth muscle contractility changes after severe burn
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/68cdfd1fdca14e978a1fbb0f617ea222
work_keys_str_mv	AT kevindespain carotidsmoothmusclecontractilitychangesaftersevereburn AT charlesrrosenfeld carotidsmoothmusclecontractilitychangesaftersevereburn AT ryanhuebinger carotidsmoothmusclecontractilitychangesaftersevereburn AT xiaofuwang carotidsmoothmusclecontractilitychangesaftersevereburn AT jaysonwjay carotidsmoothmusclecontractilitychangesaftersevereburn AT ravisradhakrishnan carotidsmoothmusclecontractilitychangesaftersevereburn AT stevenewolf carotidsmoothmusclecontractilitychangesaftersevereburn AT juquansong carotidsmoothmusclecontractilitychangesaftersevereburn
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Carotid smooth muscle contractility changes after severe burn

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