Local and systemic production of 45 cytokines in complicated proliferative diabetic retinopaty

Diabetic retinopathy (DR) is multifactorial by its origin, involving many cytokines and growth factors. Studies of cytokine levels in biological fluids seem to be relevant for an in-depth understanding of the disease pathogenesis. The purpose of the work was a comparative analysis of 45 cytokines at...

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Autores principales:	V. V. Neroev, O. V. Zaytseva, N. V. Balatskaya, A. A. Lazutova
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2020
Materias:	diabetes mellitus diabetic retinopathy tractional retinal detachment hemophthalmus iris rubeosis pathogenesis cytokines vitreous humor blood serum Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/68ce7e7cddf7495e96315de301179999
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spelling	oai:doaj.org-article:68ce7e7cddf7495e96315de3011799992021-11-18T08:03:49ZLocal and systemic production of 45 cytokines in complicated proliferative diabetic retinopaty1563-06252313-741X10.15789/1563-0625-LAS-1802https://doaj.org/article/68ce7e7cddf7495e96315de3011799992020-04-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1802https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XDiabetic retinopathy (DR) is multifactorial by its origin, involving many cytokines and growth factors. Studies of cytokine levels in biological fluids seem to be relevant for an in-depth understanding of the disease pathogenesis. The purpose of the work was a comparative analysis of 45 cytokines at systemic (blood serum (BS)) and local (vitreous humor (VH)) levels in the patients with complicated proliferative DR, showing various features of the clinical pattern. The content of cytokines was tested in 53 samples of BS and 32 samples of VH in 53 patients with type 1 and type 2 diabetes mellitus with severe proliferative DR. We used the multiplex analysis technique by means of xMAP platform and Luminex xPONENT 3.1 program using 45-plex sets (Procarta Plex «eBioscience», Austria). 25 cytokines were detected at significant amounts in BS test samples, and 27 cytokines were revealed in VH specimens. Sensitivity limits of the test system allowed to find significantly higher levels of 7 cytokines (IL-6, IL-8, IP-10, MCP-1, HGF, LIF and VEGF-A) in VH samples, than in the BS, thus indicating to their local intraocular production. Correlations between the contents of VEGF-A growth factor and amounts of cytokines, including those involved in inflammatory reactions, are shown in VH, thus presuming the interrelation of pathogenetic components, i.e., inflammation and neoangiogenesis. The features of intraocular cytokine content were determined for various manifestations of diabetic ocular changes. Hemophthalmus has been shown to be associated with increased IL-8 and IP-10; iris rubeosis, with increase in LIF; proliferative DR activity was associated with higher MCP-1 levels, and extremely severe changes were related to increase in IL-6 and EGF. Testing of cytokines in biological fluids is informative when studying the mechanisms of inflammation, neoangiogenesis, and protective responses in pathogenesis of diabetic retinopathy.V. V. NeroevO. V. ZaytsevaN. V. BalatskayaA. A. LazutovaSPb RAACIarticlediabetes mellitusdiabetic retinopathytractional retinal detachmenthemophthalmusiris rubeosispathogenesiscytokinesvitreous humorblood serumImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 22, Iss 2, Pp 301-310 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	diabetes mellitus diabetic retinopathy tractional retinal detachment hemophthalmus iris rubeosis pathogenesis cytokines vitreous humor blood serum Immunologic diseases. Allergy RC581-607
spellingShingle	diabetes mellitus diabetic retinopathy tractional retinal detachment hemophthalmus iris rubeosis pathogenesis cytokines vitreous humor blood serum Immunologic diseases. Allergy RC581-607 V. V. Neroev O. V. Zaytseva N. V. Balatskaya A. A. Lazutova Local and systemic production of 45 cytokines in complicated proliferative diabetic retinopaty
description	Diabetic retinopathy (DR) is multifactorial by its origin, involving many cytokines and growth factors. Studies of cytokine levels in biological fluids seem to be relevant for an in-depth understanding of the disease pathogenesis. The purpose of the work was a comparative analysis of 45 cytokines at systemic (blood serum (BS)) and local (vitreous humor (VH)) levels in the patients with complicated proliferative DR, showing various features of the clinical pattern. The content of cytokines was tested in 53 samples of BS and 32 samples of VH in 53 patients with type 1 and type 2 diabetes mellitus with severe proliferative DR. We used the multiplex analysis technique by means of xMAP platform and Luminex xPONENT 3.1 program using 45-plex sets (Procarta Plex «eBioscience», Austria). 25 cytokines were detected at significant amounts in BS test samples, and 27 cytokines were revealed in VH specimens. Sensitivity limits of the test system allowed to find significantly higher levels of 7 cytokines (IL-6, IL-8, IP-10, MCP-1, HGF, LIF and VEGF-A) in VH samples, than in the BS, thus indicating to their local intraocular production. Correlations between the contents of VEGF-A growth factor and amounts of cytokines, including those involved in inflammatory reactions, are shown in VH, thus presuming the interrelation of pathogenetic components, i.e., inflammation and neoangiogenesis. The features of intraocular cytokine content were determined for various manifestations of diabetic ocular changes. Hemophthalmus has been shown to be associated with increased IL-8 and IP-10; iris rubeosis, with increase in LIF; proliferative DR activity was associated with higher MCP-1 levels, and extremely severe changes were related to increase in IL-6 and EGF. Testing of cytokines in biological fluids is informative when studying the mechanisms of inflammation, neoangiogenesis, and protective responses in pathogenesis of diabetic retinopathy.
format	article
author	V. V. Neroev O. V. Zaytseva N. V. Balatskaya A. A. Lazutova
author_facet	V. V. Neroev O. V. Zaytseva N. V. Balatskaya A. A. Lazutova
author_sort	V. V. Neroev
title	Local and systemic production of 45 cytokines in complicated proliferative diabetic retinopaty
title_short	Local and systemic production of 45 cytokines in complicated proliferative diabetic retinopaty
title_full	Local and systemic production of 45 cytokines in complicated proliferative diabetic retinopaty
title_fullStr	Local and systemic production of 45 cytokines in complicated proliferative diabetic retinopaty
title_full_unstemmed	Local and systemic production of 45 cytokines in complicated proliferative diabetic retinopaty
title_sort	local and systemic production of 45 cytokines in complicated proliferative diabetic retinopaty
publisher	SPb RAACI
publishDate	2020
url	https://doaj.org/article/68ce7e7cddf7495e96315de301179999
work_keys_str_mv	AT vvneroev localandsystemicproductionof45cytokinesincomplicatedproliferativediabeticretinopaty AT ovzaytseva localandsystemicproductionof45cytokinesincomplicatedproliferativediabeticretinopaty AT nvbalatskaya localandsystemicproductionof45cytokinesincomplicatedproliferativediabeticretinopaty AT aalazutova localandsystemicproductionof45cytokinesincomplicatedproliferativediabeticretinopaty
_version_	1718422356389527552

Local and systemic production of 45 cytokines in complicated proliferative diabetic retinopaty

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