Mycelial DNA persistence in a forest soil

Abstract Living fungi can be identified and monitored in their soil habitat using an array of molecular methods. However, DNA originating from dead fungal tissue may become stabilized in the soil and remain detectable for some time after cell death rendering species detections with molecular methods...

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Autores principales:	Matthew Gordon, Kelli Van Norman
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	DNA persistence environmental DNA fungal conservation fungal DNA Phaeocollybia rare species Environmental sciences GE1-350 Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/68d5f22b83124baeaeea993fc40c842d
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spelling	oai:doaj.org-article:68d5f22b83124baeaeea993fc40c842d2021-11-23T16:15:25ZMycelial DNA persistence in a forest soil2637-494310.1002/edn3.242https://doaj.org/article/68d5f22b83124baeaeea993fc40c842d2021-11-01T00:00:00Zhttps://doi.org/10.1002/edn3.242https://doaj.org/toc/2637-4943Abstract Living fungi can be identified and monitored in their soil habitat using an array of molecular methods. However, DNA originating from dead fungal tissue may become stabilized in the soil and remain detectable for some time after cell death rendering species detections with molecular methods difficult to interpret. Very little data exist about the persistence time of DNA from dead mycelium as it decomposes within the soil ecosystem. We placed soil containing the heat‐killed mycelium of two mycorrhizal fungal species, Phaeocollybia scatesiae A.H. Sm. & Trappe and P. fallax A.H. Sm., in mesh bags and buried them at a forested site in the Oregon Coast Range. The detectability of Phaeocollybia DNA in the soil bags was monitored over 8 months using PCR with species‐specific primers. We found that after 2 months of burial, P. scatesiae was undetectable, while P. fallax was barely detected. No Phaeocollybia DNA was detected at 3 months or thereafter. With the determination of this fungal DNA persistence time, treatment effect studies with an appropriate post‐treatment waiting period can be designed. The applicability of this fungal DNA persistence time to other studies depends on many factors, but we provide a reference point for the soil/ fungus environmental DNA system where very few exist.Matthew GordonKelli Van NormanWileyarticleDNA persistenceenvironmental DNAfungal conservationfungal DNAPhaeocollybiarare speciesEnvironmental sciencesGE1-350Microbial ecologyQR100-130ENEnvironmental DNA, Vol 3, Iss 6, Pp 1208-1213 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	DNA persistence environmental DNA fungal conservation fungal DNA Phaeocollybia rare species Environmental sciences GE1-350 Microbial ecology QR100-130
spellingShingle	DNA persistence environmental DNA fungal conservation fungal DNA Phaeocollybia rare species Environmental sciences GE1-350 Microbial ecology QR100-130 Matthew Gordon Kelli Van Norman Mycelial DNA persistence in a forest soil
description	Abstract Living fungi can be identified and monitored in their soil habitat using an array of molecular methods. However, DNA originating from dead fungal tissue may become stabilized in the soil and remain detectable for some time after cell death rendering species detections with molecular methods difficult to interpret. Very little data exist about the persistence time of DNA from dead mycelium as it decomposes within the soil ecosystem. We placed soil containing the heat‐killed mycelium of two mycorrhizal fungal species, Phaeocollybia scatesiae A.H. Sm. & Trappe and P. fallax A.H. Sm., in mesh bags and buried them at a forested site in the Oregon Coast Range. The detectability of Phaeocollybia DNA in the soil bags was monitored over 8 months using PCR with species‐specific primers. We found that after 2 months of burial, P. scatesiae was undetectable, while P. fallax was barely detected. No Phaeocollybia DNA was detected at 3 months or thereafter. With the determination of this fungal DNA persistence time, treatment effect studies with an appropriate post‐treatment waiting period can be designed. The applicability of this fungal DNA persistence time to other studies depends on many factors, but we provide a reference point for the soil/ fungus environmental DNA system where very few exist.
format	article
author	Matthew Gordon Kelli Van Norman
author_facet	Matthew Gordon Kelli Van Norman
author_sort	Matthew Gordon
title	Mycelial DNA persistence in a forest soil
title_short	Mycelial DNA persistence in a forest soil
title_full	Mycelial DNA persistence in a forest soil
title_fullStr	Mycelial DNA persistence in a forest soil
title_full_unstemmed	Mycelial DNA persistence in a forest soil
title_sort	mycelial dna persistence in a forest soil
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/68d5f22b83124baeaeea993fc40c842d
work_keys_str_mv	AT matthewgordon mycelialdnapersistenceinaforestsoil AT kellivannorman mycelialdnapersistenceinaforestsoil
_version_	1718416211987922944

Mycelial DNA persistence in a forest soil

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