Anti-inflammatory Effects of a Novel Herbal Extract in the Muscle and Spinal Cord of an Amyotrophic Lateral Sclerosis Animal Model

Amyotrophic lateral sclerosis (ALS) is a complex disease characterized by motor neuron loss and muscle atrophy. There is no prominent treatment for ALS as the pathogenic process in the skeletal muscle and spinal cord is complex and multifactorial. Therefore, we investigated the effects of a herbal f...

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Autores principales:	Sun Hwa Lee, Mudan Cai, Eun Jin Yang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	amyotrophic lateral sclerosis herbal medicine oxidative stress inflammation skeletal muscle Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/68d80d7e549f453e8ad4a78d40a09f3b
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spelling	oai:doaj.org-article:68d80d7e549f453e8ad4a78d40a09f3b2021-11-11T07:08:50ZAnti-inflammatory Effects of a Novel Herbal Extract in the Muscle and Spinal Cord of an Amyotrophic Lateral Sclerosis Animal Model1662-453X10.3389/fnins.2021.743705https://doaj.org/article/68d80d7e549f453e8ad4a78d40a09f3b2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnins.2021.743705/fullhttps://doaj.org/toc/1662-453XAmyotrophic lateral sclerosis (ALS) is a complex disease characterized by motor neuron loss and muscle atrophy. There is no prominent treatment for ALS as the pathogenic process in the skeletal muscle and spinal cord is complex and multifactorial. Therefore, we investigated the effects of a herbal formula on the multi-target effects in the skeletal muscle and spinal cord in hSOD1G93A transgenic mice. We prepared a herbal extract (HE) from Glycyrrhiza uralensis, Atractylodes macrocephala Koidzumi, Panax ginseng, and Astragalus membranaceus. Control and HE-treated mice underwent rotarod and footprint tests. We also performed immunohistochemical and Western blotting analyses to assess expression of inflammation-related and oxidative stress-related proteins in the muscle and spinal cord tissues. We found that the HE increased motor activity and reduced motor neuron loss in hSOD1G93A mice. In addition, the HE significantly reduced the levels of inflammatory proteins and oxidative stress-related proteins in the skeletal muscles and spinal cord of hSOD1G93A mice. Furthermore, we demonstrated that the HE regulated autophagy function and augmented neuromuscular junction in the muscle of hSOD1G93A mice. Based on these results, we propose that the HE formula may be a potential therapeutic strategy for multi-target treatment in complex and multifactorial pathological diseases.Sun Hwa LeeMudan CaiEun Jin YangFrontiers Media S.A.articleamyotrophic lateral sclerosisherbal medicineoxidative stressinflammationskeletal muscleNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Neuroscience, Vol 15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	amyotrophic lateral sclerosis herbal medicine oxidative stress inflammation skeletal muscle Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	amyotrophic lateral sclerosis herbal medicine oxidative stress inflammation skeletal muscle Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Sun Hwa Lee Mudan Cai Eun Jin Yang Anti-inflammatory Effects of a Novel Herbal Extract in the Muscle and Spinal Cord of an Amyotrophic Lateral Sclerosis Animal Model
description	Amyotrophic lateral sclerosis (ALS) is a complex disease characterized by motor neuron loss and muscle atrophy. There is no prominent treatment for ALS as the pathogenic process in the skeletal muscle and spinal cord is complex and multifactorial. Therefore, we investigated the effects of a herbal formula on the multi-target effects in the skeletal muscle and spinal cord in hSOD1G93A transgenic mice. We prepared a herbal extract (HE) from Glycyrrhiza uralensis, Atractylodes macrocephala Koidzumi, Panax ginseng, and Astragalus membranaceus. Control and HE-treated mice underwent rotarod and footprint tests. We also performed immunohistochemical and Western blotting analyses to assess expression of inflammation-related and oxidative stress-related proteins in the muscle and spinal cord tissues. We found that the HE increased motor activity and reduced motor neuron loss in hSOD1G93A mice. In addition, the HE significantly reduced the levels of inflammatory proteins and oxidative stress-related proteins in the skeletal muscles and spinal cord of hSOD1G93A mice. Furthermore, we demonstrated that the HE regulated autophagy function and augmented neuromuscular junction in the muscle of hSOD1G93A mice. Based on these results, we propose that the HE formula may be a potential therapeutic strategy for multi-target treatment in complex and multifactorial pathological diseases.
format	article
author	Sun Hwa Lee Mudan Cai Eun Jin Yang
author_facet	Sun Hwa Lee Mudan Cai Eun Jin Yang
author_sort	Sun Hwa Lee
title	Anti-inflammatory Effects of a Novel Herbal Extract in the Muscle and Spinal Cord of an Amyotrophic Lateral Sclerosis Animal Model
title_short	Anti-inflammatory Effects of a Novel Herbal Extract in the Muscle and Spinal Cord of an Amyotrophic Lateral Sclerosis Animal Model
title_full	Anti-inflammatory Effects of a Novel Herbal Extract in the Muscle and Spinal Cord of an Amyotrophic Lateral Sclerosis Animal Model
title_fullStr	Anti-inflammatory Effects of a Novel Herbal Extract in the Muscle and Spinal Cord of an Amyotrophic Lateral Sclerosis Animal Model
title_full_unstemmed	Anti-inflammatory Effects of a Novel Herbal Extract in the Muscle and Spinal Cord of an Amyotrophic Lateral Sclerosis Animal Model
title_sort	anti-inflammatory effects of a novel herbal extract in the muscle and spinal cord of an amyotrophic lateral sclerosis animal model
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/68d80d7e549f453e8ad4a78d40a09f3b
work_keys_str_mv	AT sunhwalee antiinflammatoryeffectsofanovelherbalextractinthemuscleandspinalcordofanamyotrophiclateralsclerosisanimalmodel AT mudancai antiinflammatoryeffectsofanovelherbalextractinthemuscleandspinalcordofanamyotrophiclateralsclerosisanimalmodel AT eunjinyang antiinflammatoryeffectsofanovelherbalextractinthemuscleandspinalcordofanamyotrophiclateralsclerosisanimalmodel
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Anti-inflammatory Effects of a Novel Herbal Extract in the Muscle and Spinal Cord of an Amyotrophic Lateral Sclerosis Animal Model

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