Genotypic variability and relationships of Sorghum bicolor accessions from Java Island, Indonesia based on IRAP markers

Abstract. Martiwi INA, Nugroho LH, Daryono BS, Susandarini R. 2020. Genotypic variability and relationships of Sorghum bicolor accessions from Java Island, Indonesia based on IRAP markers. Biodiversitas 21: 5637-5643. Sorghum (Sorghum bicolor (L.) Moench) is a multifunctional crop with high adaptabi...

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Autores principales:	Ika Nugraheni Ari Martiwi, Laurentius Hartanto Nugroho, Budi Setiadi Daryono, Ratna Susandarini
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	genotype, molecular characterization, retrotransposon, sorghum, taxonomic affinity Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/68dc10889d974f27894677b30b26435a
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spelling	oai:doaj.org-article:68dc10889d974f27894677b30b26435a2021-11-22T00:52:01ZGenotypic variability and relationships of Sorghum bicolor accessions from Java Island, Indonesia based on IRAP markers1412-033X2085-472210.13057/biodiv/d211220https://doaj.org/article/68dc10889d974f27894677b30b26435a2020-11-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7066https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Martiwi INA, Nugroho LH, Daryono BS, Susandarini R. 2020. Genotypic variability and relationships of Sorghum bicolor accessions from Java Island, Indonesia based on IRAP markers. Biodiversitas 21: 5637-5643. Sorghum (Sorghum bicolor (L.) Moench) is a multifunctional crop with high adaptability to various habitats and thus it is important to assess the genotypic variability of this crop species. This study aimed to reveal the genotypic variability and relationships of sorghum accessions using inter-retrotransposon amplified polymorphism (IRAP) molecular markers. Twenty-three sorghum accessions were collected from smallholder farmers in three provinces of Java Island during the 2019 planting season. The IRAP profiles generated using 10 primer combinations produced 233 scorable bands, with degree of polymorphism 75-96.9%. Cluster analysis using the neighbor-joining (NJ) method showed the formation of three groups corresponds to geographic origin of samples. The NJ dendrogram structure indicated high genotypic variability, and was supported by distribution pattern of accessions on the scatterplot constructed using principal coordinate analysis. The grouping of accessions did not correspond to classification of botanical races. Each group recognized from cluster analysis and principal coordinate analysis consisted of various races namely Bicolor, Guinea, Caudatum, and Kaffir. The fact that each group consisted of various botanical races indicated local adaptation and the maintenance of genotypic identity through farmers’ selection in traditional cultivation practices. It could be concluded, therefore, that geographical proximity and the practice of seed exchange between different regions showed considerable influence on the genotypic relationships of sorghum accessions.Ika Nugraheni Ari MartiwiLaurentius Hartanto NugrohoBudi Setiadi DaryonoRatna SusandariniMBI & UNS Soloarticlegenotype, molecular characterization, retrotransposon, sorghum, taxonomic affinityBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	genotype, molecular characterization, retrotransposon, sorghum, taxonomic affinity Biology (General) QH301-705.5
spellingShingle	genotype, molecular characterization, retrotransposon, sorghum, taxonomic affinity Biology (General) QH301-705.5 Ika Nugraheni Ari Martiwi Laurentius Hartanto Nugroho Budi Setiadi Daryono Ratna Susandarini Genotypic variability and relationships of Sorghum bicolor accessions from Java Island, Indonesia based on IRAP markers
description	Abstract. Martiwi INA, Nugroho LH, Daryono BS, Susandarini R. 2020. Genotypic variability and relationships of Sorghum bicolor accessions from Java Island, Indonesia based on IRAP markers. Biodiversitas 21: 5637-5643. Sorghum (Sorghum bicolor (L.) Moench) is a multifunctional crop with high adaptability to various habitats and thus it is important to assess the genotypic variability of this crop species. This study aimed to reveal the genotypic variability and relationships of sorghum accessions using inter-retrotransposon amplified polymorphism (IRAP) molecular markers. Twenty-three sorghum accessions were collected from smallholder farmers in three provinces of Java Island during the 2019 planting season. The IRAP profiles generated using 10 primer combinations produced 233 scorable bands, with degree of polymorphism 75-96.9%. Cluster analysis using the neighbor-joining (NJ) method showed the formation of three groups corresponds to geographic origin of samples. The NJ dendrogram structure indicated high genotypic variability, and was supported by distribution pattern of accessions on the scatterplot constructed using principal coordinate analysis. The grouping of accessions did not correspond to classification of botanical races. Each group recognized from cluster analysis and principal coordinate analysis consisted of various races namely Bicolor, Guinea, Caudatum, and Kaffir. The fact that each group consisted of various botanical races indicated local adaptation and the maintenance of genotypic identity through farmers’ selection in traditional cultivation practices. It could be concluded, therefore, that geographical proximity and the practice of seed exchange between different regions showed considerable influence on the genotypic relationships of sorghum accessions.
format	article
author	Ika Nugraheni Ari Martiwi Laurentius Hartanto Nugroho Budi Setiadi Daryono Ratna Susandarini
author_facet	Ika Nugraheni Ari Martiwi Laurentius Hartanto Nugroho Budi Setiadi Daryono Ratna Susandarini
author_sort	Ika Nugraheni Ari Martiwi
title	Genotypic variability and relationships of Sorghum bicolor accessions from Java Island, Indonesia based on IRAP markers
title_short	Genotypic variability and relationships of Sorghum bicolor accessions from Java Island, Indonesia based on IRAP markers
title_full	Genotypic variability and relationships of Sorghum bicolor accessions from Java Island, Indonesia based on IRAP markers
title_fullStr	Genotypic variability and relationships of Sorghum bicolor accessions from Java Island, Indonesia based on IRAP markers
title_full_unstemmed	Genotypic variability and relationships of Sorghum bicolor accessions from Java Island, Indonesia based on IRAP markers
title_sort	genotypic variability and relationships of sorghum bicolor accessions from java island, indonesia based on irap markers
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/68dc10889d974f27894677b30b26435a
work_keys_str_mv	AT ikanugraheniarimartiwi genotypicvariabilityandrelationshipsofsorghumbicoloraccessionsfromjavaislandindonesiabasedonirapmarkers AT laurentiushartantonugroho genotypicvariabilityandrelationshipsofsorghumbicoloraccessionsfromjavaislandindonesiabasedonirapmarkers AT budisetiadidaryono genotypicvariabilityandrelationshipsofsorghumbicoloraccessionsfromjavaislandindonesiabasedonirapmarkers AT ratnasusandarini genotypicvariabilityandrelationshipsofsorghumbicoloraccessionsfromjavaislandindonesiabasedonirapmarkers
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Genotypic variability and relationships of Sorghum bicolor accessions from Java Island, Indonesia based on IRAP markers

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